Rabbit anti eif4h

Manufactured by Cell Signaling Technology

Rabbit anti-eIF4H is a primary antibody that specifically detects eIF4H (eukaryotic translation initiation factor 4H) in various sample types. eIF4H is a protein involved in the regulation of protein synthesis initiation.

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Lab products found in correlation

Mouse anti ncl, by Santa Cruz Biotechnology (1 mentions) Mouse anti hnrnpc1 c2, by Abcam (1 mentions) Rabbit anti h3, by Cell Signaling Technology (1 mentions) Mouse anti strep, by Qiagen (1 mentions) Rabbit anti flag, by Merck Group (1 mentions) Rabbit anti ncl, by Abcam (1 mentions) Hrp conjugated goat anti mouse or goat anti rabbit secondary antibodies, by Southern Biotech (1 mentions) Luminata classico western hrp substrate, by Merck Group (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Mouse anti flag, by Merck Group (1 mentions)

Close spelling variants of this product

EIF4H (3 citations)

2 protocols using rabbit anti eif4h

Western Blot Analysis of Nuclear Proteins

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Cell lysates were prepared in lysis buffer and quantified by Bradford assay. Equivalent amounts of each sample were resolved by SDS-PAGE and Western blotted with the following antibodies: Rabbit anti-NCL (Abcam), Mouse anti-NCL (Santa Cruz), Rabbit anti-eIF4H (Cell signaling). Rabbit anti-Flag (Sigma), Mouse anti hnRNPC1/C2 (Abcam), Rabbit anti-H3 (Cell Signaling), Rabbit anti-Xrn1 (Sigma), Mouse anti-Strep (Qiagen). Primary antibodies were followed by HRP-conjugated goat anti-mouse or goat anti-rabbit secondary antibodies (Southern Biotechnology, 1:5000).

Muller M., Hutin S., Marigold O., Li K.H., Burlingame A, & Glaunsinger B.A. (2015). A Ribonucleoprotein Complex Protects the Interleukin-6 mRNA from Degradation by Distinct Herpesviral Endonucleases. PLoS Pathogens, 11(5), e1004899.

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Western Blot Analysis of Drosha

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Protein was collected from cells transiently transfected with Lipofectamine 2000 (Invitrogen) alone, FLAG-Tagged GFP, pcK-FLAG-Drosha or pCK-FLAG-TN Drosha in 2X Laemmli sample buffer. Proteins were separated on a 5% stacking, 12% resolving SDS-PAGE gels and transferred to FL-Immobilon membrane (Millipore) and probed with rabbit-anti-eIF4H (Cell Signaling) or Mouse-Anti-FLAG (Sigma). β-Actin was detected with mouse-anti-β-actin (Sigma). Proteins were visualized with Luminata Classico western HRP substrate (Millipore). Quantitation was preformed using NIH ImageJ software.

Havens M.A., Reich A.A, & Hastings M.L. (2014). Drosha Promotes Splicing of a Pre-microRNA-like Alternative Exon. PLoS Genetics, 10(5), e1004312.

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