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4 protocols using thiacloprid

1

Quantitative Analysis of Neonicotinoid Pesticides

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Clothianidin, dinotefuran, imidacloprid, acetamiprid, N-dm acetamiprid and thiacloprid were purchased from Kanto Chemical (Tokyo, Japan). Nitenpyram was purchased from Wako Pure Chemical Industries (Osaka, Japan). Clothianidin-d3, dinotefuran-d3, thiacloprid-d4, Nitenpyram-d3, acetamiprid-d3, imidacloprid-d4, N-dm-acetamiprid-d3 and Thiamethoxam-d4 were purchased from Sigma-Aldrich (St. Louis, MO). Thiamethoxam was purchased from Dr. Ehrenstorfer (Augsburg, Germany). All reagents and solvents used in the current study were of analytical grade; and they were purchased from Kanto Chemical Co., Inc. (Chuo-Ku, Tokyo, Japan).
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2

Neonicotinoid Metabolites Identification

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Acetamiprid, dinotefuran, imidacloprid, and thiacloprid were purchased from Kanto Chemical (Tokyo, Japan). Clothianidin and N-desmethyl-Thiamethoxam were purchased from Fluka (Buchs, Switzerland). Nitenpyram, CPMA, CPMF, and CPF were purchased from Wako Pure Chemical Industries (Osaka, Japan). Clothianidin-d3, dinotefuran-d3, imidacloprid-d4, thiacloprid-d4, Thiamethoxam-d4, N-desmethyl-Acetamiprid and N-desnitro-imidacloprid were purchased from Sigma-Aldrich (St. Louis, MO). Hydroxy-imidacloprid was purchased from Hypha Discovery (Uxbridge, UK). Acetamiprid-d6 and Nitenpyram-d3 were purchased from Hayashi Junyaku (Osaka, Japan). Thiamethoxam was purchased from Dr. Ehrenstorfer (Augsburg, Germany). Other metabolites (N-acetyl-Acetamiprid, N-acetyl-desmethyl-Acetamiprid, N-descyano-Acetamiprid, N-desmethyl-clothianidin, N-desmethyl-clothianidin urea, N-desnitro-clothianidin, clothianidin urea, N-desmethyl-desnitro-clothianidin, N-desmethyl-dinotefuran, dinotefuran-urea, N-desmethyl-Nitenpyram, thiacloprid-amide, N-descyano-dehydro-thiacloprid) were synthesized at Toho University (Supplement).
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3

Neonicotinoid Pesticide Quantification

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Acetamiprid, dinotefuran, imidacloprid, nitenpyram and thiacloprid were purchased from Kanto Chemical Corp.
(Tokyo, Japan). Thiamethoxam was purchased from Dr. Ehrenstorfer. Clothianidin, Clothianidin-d3, dinotefuran-d3, imidacloprid-d4, thiacloprid-d4, Thiamethoxam-d4, and N-desmethylAcetamiprid (DMAP) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetamiprid-d6 and nitenpyram-d3 were purchased from Hayashi Pure Chemical Ind. (Osaka, Japan). Acetonitrile, dichloromethane formic acid, ammonium acetate and distilled water were all HPLC grade and were purchased from Kanto Chemical.
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4

Quantification of Neonicotinoid Pesticides

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Acetamiprid, dinotefuran, imidacloprid, nitenpyram and thiacloprid were purchased from Kanto Chemical Corp. (Tokyo, Japan). Thiamethoxam was purchased from Dr. Ehrenstorfer. Clothianidin, Clothianidin-d3, dinotefuran-d3, imidacloprid-d4, thiacloprid-d4, Thiamethoxam-d4, and N-desmethylAcetamiprid (DMAP) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetamiprid-d6 and nitenpyram-d3 were purchased from Hayashi Pure Chemical Ind. (Osaka, Japan). Acetonitrile, dichloromethane formic acid, ammonium acetate and distilled water were all HPLC grade and were purchased from Kanto Chemical.
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