Cells were fixed with 100 μL of FOXP3 Fix/Perm Buffer (BioLegend) over night at 4°C. The fixed and permeabilized cells were stained with mouse anti-human CCL-4/MIP-1 beta PerCP conjugated monoclonal antibody or mouse IgG2B-PerCP isotype control (R&D Systems). All samples were acquired and analyzed on BD Accuri™ C6 Flow Cytometer (BD Biosciences).
Mouse igg2b percp isotype control
Manufactured by R&D Systems
Sourced in United States
The Mouse IgG2B-PerCP Isotype Control is a control reagent used in flow cytometry experiments. It is a mouse immunoglobulin G2B (IgG2B) antibody conjugated to the PerCP fluorescent dye. This isotype control can be used to establish background fluorescence levels and set appropriate gates when analyzing cell populations by flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Foxp3 fix perm buffer, by BioLegend (1 mentions)
Accuri c6 flow cytometer, by BD (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Cd69 pe, by BioLegend (1 mentions)
Bamhi, by New England Biolabs (1 mentions)
Anti cd14 pacific blue, by BioLegend (1 mentions)
Anti cd62l fitc, by BioLegend (1 mentions)
Cd16 fitc, by BioLegend (1 mentions)
Anti pd 1 apc, by BioLegend (1 mentions)
Hindiii, by New England Biolabs (1 mentions)
Bsteii, by New England Biolabs (1 mentions)
Puromycin, by Merck Group (1 mentions)
Xhoi, by New England Biolabs (1 mentions)
2 protocols using mouse igg2b percp isotype control
1
Quantifying Cellular CCL-4/MIP-1 Beta
2
T-cell Activation Assay Protocol
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Antibodies against CD3 and CD28-coupled magnetic beads (anti-CD3/28), penicillin, and streptomycin were from Invitrogen (Grand Island, NY, USA). Anti-CD62L-FITC, anti-CD45RA-TC, anti-CD14-Pacific Blue, anti-PD-1-APC, anti-HLA-DR-Alexa Fluor® 700, CD69-PE, and CD16-FITC were from Biolegend (San Diego, CA, USA). Anti-human CCL4-PerCP and its mouse IgG2B-PerCP isotype control were from R&D systems (Minneapolis, MN, USA). HindIII,BamHI,XhoI,NotI, and BstEII were from New England BioLabs (Ipswich, MA, USA). Puromycin and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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