Eosin methylene blue (EMB), thioglycollate broth, blood agar, barium chloride, sulfuric acid, crystal violate, safranin, lugol solution, acetone, ethanol, oxidase, and catalase reagents were purchased from Merck (Germany). API20E kit was obtained from Biomerieux (France). All other chemicals were analytic grade and of commercially available.
Oxidase
Manufactured by Merck Group
Sourced in Germany
Oxidase is a type of laboratory equipment used to detect the presence of oxidase enzymes in biological samples. It functions by catalyzing the oxidation of specific substrates, which can be used to identify certain bacterial species. The oxidase test provides a rapid and simple method for evaluating the oxidative capabilities of microorganisms.
Automatically generated - may contain errors
Lab products found in correlation
Catalase, by Merck Group (3 mentions)
Thioglycollate broth, by Merck Group (2 mentions)
Blood agar, by Merck Group (2 mentions)
Barium chloride, by Merck Group (2 mentions)
Crystal violate, by Merck Group (2 mentions)
Safranin, by Merck Group (2 mentions)
Lugol solution, by Merck Group (2 mentions)
Eosin methylene blue, by Merck Group (2 mentions)
Api 20e kit, by bioMérieux (2 mentions)
Ethanol, by Merck Group (2 mentions)
Sulfuric acid, by Merck Group (2 mentions)
Acetone, by Merck Group (2 mentions)
Bromothymol blue, by Merck Group (1 mentions)
Agar, by Thermo Fisher Scientific (1 mentions)
Sodium molybdate, by Merck Group (1 mentions)
Acetic acid, by Thermo Fisher Scientific (1 mentions)
Genejet genomic dna purification kit, by Thermo Fisher Scientific (1 mentions)
Cellulase from trichoderma reesei atcc 26921, by Merck Group (1 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
Potassium dihydrogen phosphate, by Merck Group (1 mentions)
6 protocols using oxidase
1
Bacterial Identification Techniques
2
Kombucha SCOBY Cultivation Protocol
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Kombucha SCOBY (symbiotic colony of bacteria and yeast) was obtained from Sri Dhanvanthiri Probiotics Ltd., Kodaikanal, India (True Brew Kombucha tea). Sodium chloride, sodium molybdate, potassium chloride, di sodium hydrogen phosphate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, calcium carbonate, calcium chloride, magnesium sulfate, disodium hydrogen phosphate, citric acid, bromothymol blue and oxidase test disks (product no: 70439) were purchased from Merck (Darmstadt, Germany). Acetic acid and agar were purchased from Fisher Scientific (Loughborough, UK). Glucose and casein amino acids were purchased from VWR International (Leuven, Belgium). Tryptone, peptone and yeast extract were from Lab M Limited (Heywood, UK). Ethanol was from Altia Oyj (Helsinki, Finland). Cycloheximide (Product no: C7698), and cellulase from Trichoderma reesei ATCC 26921 (product no: C2730) was purchased from Sigma-Aldrich (St. Louis, MO, USA). GeneJET Genomic DNA Purification Kit was purchased from Thermo Scientific (Waltham, MA, USA). Crude glycerol was generously provided by Perstorp AB (Malmo, Sweden).
3
Microbial Identification Assay
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Eosin methylene blue, thioglycollate broth, blood agar, barium chloride, sulfuric acid, crystal violate, Safranin, lugol solution, acetone, ethanol, oxidase and catalase reagents were purchased from Merck (Germany). API20E kit was obtained from Biomerieux (France). All other chemicals were commercially available.
4
Identifying Helicobacter pylori Presence
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5
Pseudomonas Identification from Burn Wounds
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For sampling, wound swabs were obtained from the burn patients and P. aeruginosa was identified by standard microbiological methods including Gram stains, culture on mediums such as blood agar, MacConkey agar, triple sugar iron agar (TSI), cetrimide agar (Merck, UK), oxidase (Sigma, USA), catalase, oxidative/fermentative (OF), indole, methyl red (MR), Voges-Proskauer (VP) tests (Merck, UK), and growth at 42°C.
6
Comprehensive Bacterial Phenotypic Characterization
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Phenotypic identification of isolated bacterial strain was performed by morphological, cultural and biochemical characters examination, according with Christensen et al. 2003 (link), Christensen et al. 2007 (link), Rzewuska et al., 2007 , Bisgaard et al., 2009 (link) and ABIS on line software, Stoica and Sorescu, 2020. Cell morphology was observed in Gramstaining slides, mobility was appreciated in semisolid medium (Mobility-Indol-Urea medium) and cultural characters were investigated with blood nutrient agar and nutrient broth with serum and glucose. Biochemical characters of the isolated strain were determined using MIU, TSI (Triple-Sugar-Iron) and Simmon's citrate media, API 20 E, API 20 NE, API STAPH and API ZYM tests (bioMerieux, France), according to the manufacturers instructions. The catalase (3% H 2 O), oxidase (Sigma) and ONPG (Oxoid) tests were performed, also.
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