Anti lamin a

Western blot analysis was performed according to a standard protocol. The following antibodies were used to detect each target protein: anti-LDHA (3582 S, Cell Signaling, Danvers, MA, USA), anti-Lamin A (sc-71481), anti-GAPDH (sc-31915), anti-Cytochrome C (sc-13560), anti-β-actin (sc-47778, Santa Cruz, Dallas, TX, USA), anti-Histone H4 (ab10158, Abcam, Cambridge, UK), anti-acetyl-histone H4 (#06-866, Merck, Kenilworth, NJ, USA), anti-mouse (sc-2005), anti-rabbit (sc-2004), and anti-goat IgG-HRP (sc-2922, Santa Cruz, Dallas, TX, USA) antibodies.

MG132 was purchased from Selleckchem. Cycloheximide was purchased from Calbiochem. Phos-tag acrylamide was purchased from Wako. The TIAamp Virus RNA kit obtained from Tiangen was used to extract RNA from plasma. The following primary antibodies were used: anti-SDH5 (Cell Signaling Technology, USA), anti-P53 (Santa Cruz Biotechnology, USA), anti-MDM2 (Abcam, UK), anti-P21 (Cell Signaling Technology, USA), anti-γH2AX (Cell Signaling Technology, USA), anti-H2AX (Cell Signaling Technology, USA), anti-Noxa (Cell Signaling Technology, USA), anti-BCL2 (Cell Signaling Technology, USA), anti-BAX (Cell Signaling Technology, USA), anti-PUMA (Cell Signaling Technology, USA), anti-Caspase9 (Cell Signaling Technology, USA), anti-Cleaved Caspase 9 (Cell Signaling Technology, USA), anti-Caspase3 (Cell Signaling Technology, USA), anti-Cleaved Caspase3 (Cell Signaling Technology, USA), anti-DNA-PKcsThr2609 (ABclonal Biotech Co., China), anti-DNA-PKcs (ABclonal Biotech Co., China), anti-ku70 (ABclonal Biotech Co., China), anti-ku86 (ABclonal Biotech Co., China), anti-HA (Cell Signaling Technology, USA), anti-flag (Santa Cruz Biotechnology, USA), anti-GAPDH (Abcam, UK),anti-Actin (Cell Signaling Technology, USA), and anti-laminA (Santa Cruz Biotechnology, USA).

Proteins were extracted using RIPA buffer (Thermo Fisher Scientific, Waltham, MA, USA), and the protein content was measured using the DC Protein Assay Kit (BioRad, Hercules, CA, USA). Then, 20–40 μg of total protein were resolved by SDS-PAGE electrophoresis and transferred onto a PVDF membrane. After blocking, membranes were incubated with the following primary antibodies: Anti-DKK-1 (diluted 1:250, cell signaling, Danvers, MA, USA, 4687S), Anti-c-Myc (diluted 1:100, Santa Cruz Biotechnology, Franklin Lakes, NJ, USA, sc-40), Anti-MYOD1 (diluted 1:1000, Abcam, Cambridge, UK, ab16148), Anti-Myogenin (diluted 1:250, BD Pharmingen, Franklin Lakes, NJ, USA, 556358), Anti-FAK (diluted 1:1000, cell signaling, Danvers, MA, USA, 3285S), Anti-β-catenin (diluted 1:1000, BD Bioscience, Franklin Lakes, NJ, USA, 610154), Anti-Lamin A (diluted 1:1000, Santa Cruz Biotechnology, Franklin Lakes, NJ, USA, sc-20681) and Anti-Actin (diluted 1:10,000, Santa Cruz Biotechnology, Franklin Lakes, NJ, USA, sc-1616). Anti-goat (diluted 1:2000, Dako, Glostrup, Denmark, P0449), anti-mouse (diluted 1:2000, Dako, Glostrup, Denmark, P0260) and anti-rabbit (diluted 1:5000, Sigma, Saint louis, MO, USA, A0545) were used as secondary antibodies. Uncropped membranes and values of intensity ratio for each band are shown in Supplementary Figure S1.

Mouse monoclonal (anti-phosphor ERK, p-ERK) and rabbit polyclonal (anti-ERK, anti-RhoA, anti-lamin A, anti-ROCK1, anti-mDia, anti-α-tubulin, anti-E-cadherin) and goat polyclonal anti-p-MYPT1(Thr 853) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Mouse monoclonal anti-threonine, anti-serine antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA). Rabbit anti-GFP antibody, was from Clonetech (Mountain View, CA). Purified His tagged RhoA protein was from Cytoskeleton Inc. (Denver, CO). Purified active ERK1 was purchased from SignalChem (Richmond, BC, Canada). U0126, Y-27632, Glutathione cross-linked to 4% agarose, goat anti-mouse IgG conjugated with agarose, protein A conjugated with agarose, and Amino Amido Black staining solution were purchased from Sigma-Aldrich (St. Louis, MO). Unless otherwise specified, all chemicals were purchased from Sigma-Aldrich.