Chiralcel oj h column

Manufactured by Daicel

Sourced in Japan

The Chiralcel OJ-H column is a type of chromatography column used for the separation and purification of enantiomers. It is a high-performance liquid chromatography (HPLC) column that utilizes a chiral stationary phase to selectively retain and separate the different optical isomers of a compound.

Automatically generated - may contain errors

Lab products found in correlation

Prominence uflc liquid chromatography system, by Shimadzu (1 mentions) Hp 1100 hplc, by Agilent Technologies (1 mentions) Cp chirasil dex cb column, by Agilent Technologies (1 mentions) Hp 5 column, by Agilent Technologies (1 mentions) Db 5 column, by Agilent Technologies (1 mentions) 7890a gc system, by Agilent Technologies (1 mentions)

Close spelling variants of this product

CHIRALCEL OJ-H (3 citations)

6 protocols using chiralcel oj h column

Enantioselective Synthesis of Chiral Ester

Check if the same lab product or an alternative is used in the 5 most similar protocols

[α]_D²⁰ = −21.5 (c 0.14,
CH₂Cl₂). ¹H NMR (400 MHz, CDCl₃) δ 7.38–7.31
(m, 2H), 7.07–6.99 (m, 2H), 5.14–5.06 (m, 1H), 4.18
(q, J = 7.1 Hz, 2H), 3.39 (d, J =
2.6 Hz, 1H), 2.76–2.64 (m, 2H), 1.26 (t, J = 7.1 Hz, 3H). ¹³C NMR (100 MHz, CDCl₃) δ
172.3, 162.3 (d, J = 244 Hz), 138.3, 127.4, 115.3,
69.7, 60.9, 43.3, 14.1. The enantiomeric excess was determined by
HPLC using the Daicel Chiralcel OJ-H column, hexane/i-PrOH 99:1, flow rate 1.0 mL/min, UV detection at 220 nm, t_minor = 57.433 min, t_major = 59.097 min, 82% ee.

Ouyang L., Xia Y., Wei Y., Liao J, & Luo R. (2020). Highly Catalytic Enantioselective Reformatsky Reaction with Aldehydes and Ketones Using an Available Prolinol Ligand. ACS Omega, 5(27), 16967-16975.

+ Open protocol

+ Expand

Stereoselective CYP3201B1 Biotransformation

Check if the same lab product or an alternative is used in the 5 most similar protocols

A 400‐μL reaction mixture containing 500 nm of CYP3201B1, 50 mm KPB (pH 6.0), 2 mm NADPH, and 1 mm PAN was incubated at 20 °C for 10 min. The reaction was started by the addition of NADPH. Reaction products were extracted using 600 μL of n‐hexane:2‐propanol (85 : 15) and analysed using a Prominence UFLC Liquid Chromatography System (Shimadzu) equipped with a CHIRALCEL OJ‐H column (250 mm × 4.6 mm i.d.; Daicel Corporation, Tokyo, Japan) under the following conditions: Column oven temperature of 30 °C, solvent of n‐hexane:2‐propanol (85 : 15) and flow rate of 1.0 mL·min⁻¹.

Yamaguchi T., Kuwahara Y, & Asano Y. (2017). A novel cytochrome P450, CYP3201B1, is involved in (R)‐mandelonitrile biosynthesis in a cyanogenic millipede. FEBS Open Bio, 7(3), 335-347.

+ Open protocol

+ Expand

Chiral HPLC Analysis of ZPF Stereoisomers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chiral HPLC analysis was carried out on an Agilent HP 1100 HPLC using a Chiralcel OJ-H column (4.6 mm × 150 mm, 5 µm, Daicel Corp., Tokyo, Japan). The mobile phase, hexane-isopropanol-triflouroacetic acid (90:10:0.1, v/v/v), was filtered (0.45 µm), degassed, and delivered at a flow rate of 0.8 ml/min. The column temperature was maintained at 25 °C, and the injection volume was 20 µl. (RS)-ZPF, (R)-ZPF, and (S)-ZPF were detected at 240 nm.

Pham C.V., Baek J.S., Park J.H., Jung S.H., Kang J.S, & Cho C.W. (2018). A thorough analysis of the effect of surfactant/s on the solubility and pharmacokinetics of (S)-zaltoprofen. Asian Journal of Pharmaceutical Sciences, 14(4), 435-444.

+ Open protocol

+ Expand

HPLC-based Determination of Reaction Conversion and Enantiomeric Excess

Check if the same lab product or an alternative is used in the 5 most similar protocols

The reactions were terminated by adding acetonitrile. Subsequently, the supernatant was filtered via 0.22 μm filtration after the high‐speed centrifugation (13,000 rpm, 10 min). The samples were analyzed by using the HPLC [Cosmosil‐C18, flow rate: 1ml/min, column temperature: 28°C, UV detection wavelength: 280 nm, mobile phase: isocratic elution with acetonitrile (A): water (B)‐60:40(v/v)]. The conversion rate was determined as the product concentration /(substrate concentration)/100%. The product concentration or substrate concentration was calculated from the standard curve. Values were expressed as the means ± SD (n = 3).
The e.e values were analyzed by using the chiral HPLC that was connected to a CHIRALCEL OJ‐H column (Daicel Chemical Industries, Ltd., Tokyo, Japan). The mobile phase was n‐hexane: ethanol‐60:40 (v/v). The e.e values were determined with the formula 100%*(S‐R)/(S + R).

Li B., Xiao M., Dong X, & Huang Z. (2022). An improved whole‐cell biotransformation system for (S)‐equol production. Food Science & Nutrition, 10(7), 2318-2324.

+ Open protocol

+ Expand

Analytical Methods for Chiral Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

GC analyses were performed on a 7890A GC system (Agilent), equipped with an FID detector. HPLC analyses were carried on a 1100A HPLC system (Agilent), equipped with an VWD detector. The product yield and ee value of (R)-levodione were determined by GC using a CP-ChiraSil-DEX CB column (25 m × 0.25 mm × 0.25 μm, Agilent) as described previously.^{3,11 (link)} The product yield and de value of dihydrocarvone were determined by GC using a DB-5 column (30 m × 0.32 mm × 0.25 μm, Agilent) as described previously.^{3 (link)} The yield and ee value of 2-methyl-hydrocinnamaldehyde (12b) was determined by HPLC using a Chiralcel OJ-H column (250 mm × 4.6 mm, Daicel, Shanghai, China) as described previously.^{11 (link)} The conversion of citral (12a) was performed on GC using a HP-5 column (30 m × 0.32 mm × 0.25 μm, Agilent) as described previously.^{3 (link)} The ee value of citronellal was determined by GC using a Beta DEX 225 column (30 m × 0.25 mm × 0.25 μm, Supelco, Bellafonte, PA, USA) as described previously.^{3 (link)}

Zhang B., Sun J., Zheng Y., Mao X., Lin J, & Wei D. (2022). Identification of a novel ene reductase from Pichia angusta with potential application in (R)-levodione production. RSC Advances, 12(22), 13924-13931.

+ Open protocol

+ Expand

Enantiopurification of Racemate by SFC

Check if the same lab product or an alternative is used in the 5 most similar protocols

S006-830
racemate was synthesized as described earlier^{4 (link)} (Supporting Information, Figure S1).
The enantiomers were purified by supercritical fluid chromatography
on a chiral column (Chiralcel OJ-H column, Daicel Corp., Japan). The
optimized protocol comprised a mobile phase of 0.5% isopropanol in
methanol, with a flow rate of 2.5 mL/min, temperature of 35 °C,
and detection wavelength of 233 nm.

Singh P., Kumar S.K., Maurya V.K., Mehta B.K., Ahmad H., Dwivedi A.K., Chaturvedi V., Thakur T.S, & Sinha S. (2017). S-Enantiomer of the Antitubercular Compound S006-830 Complements Activity of Frontline TB Drugs and Targets Biogenesis of Mycobacterium tuberculosis Cell Envelope. ACS Omega, 2(11), 8453-8465.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!