0.20% w/w copolymer dispersions were prepared at 20 °C. Copper/palladium TEM grids (Agar Scientific, UK) were surface-coated in-house to produce a thin film of amorphous carbon, then plasma glow-discharged for 30 seconds to create a hydrophilic surface. Droplets of freshly-prepared aqueous copolymer dispersions (9 μL; 0.20% w/w) were placed on a hydrophilic grid for 1 min and then blotted with filter paper to remove excess solution. The deposited nanoparticles were then negatively stained with an aqueous solution of uranyl formate (9 μL; 0.75% w/w) for a further 20 seconds, then carefully blotted to remove excess stain and dried with a vacuum hose. TEM grids were imaged using a FEI Tecnai Spirit TEM instrument equipped with a Gatan 1kMS600CW CCD camera operating at 120 kV.
Tecnai spirit tem instrument
Manufactured by Ametek
The Tecnai Spirit TEM (Transmission Electron Microscope) is a lab equipment instrument designed for high-resolution imaging and analysis of materials at the nanoscale. Its core function is to provide detailed structural and compositional information about samples using a focused beam of electrons.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using tecnai spirit tem instrument
1
Transmission Electron Microscopy of Copolymer Nanoparticles
2
Copolymer Nanoparticle Characterization by TEM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Copolymer dispersions
were diluted 50-fold at 20 °C to generate 0.20% w/w dispersions.
Copper/palladium TEM grids (Agar Scientific, UK) were coated in-house
to produce a thin film of amorphous carbon. These grids were then
treated with a plasma glow discharge for 30 s to create a hydrophilic
surface. One droplet of each aqueous diblock copolymer dispersion
(12 μL; 0.20% w/w) was placed on a freshly treated grid for
1 min and then blotted with filter paper to remove excess solution.
To stain the deposited nanoparticles, an aqueous solution of uranyl
formate (9 μL; 0.75% w/w) was placed on the sample-loaded grid
via micropipet for 20 s and then carefully blotted to remove excess
stain. Each grid was then carefully dried using a vacuum hose. Imaging
was performed using a FEI Tecnai Spirit TEM instrument equipped with
a Gatan 1kMS600CW CCD camera operating at 120 kV.
were diluted 50-fold at 20 °C to generate 0.20% w/w dispersions.
Copper/palladium TEM grids (Agar Scientific, UK) were coated in-house
to produce a thin film of amorphous carbon. These grids were then
treated with a plasma glow discharge for 30 s to create a hydrophilic
surface. One droplet of each aqueous diblock copolymer dispersion
(12 μL; 0.20% w/w) was placed on a freshly treated grid for
1 min and then blotted with filter paper to remove excess solution.
To stain the deposited nanoparticles, an aqueous solution of uranyl
formate (9 μL; 0.75% w/w) was placed on the sample-loaded grid
via micropipet for 20 s and then carefully blotted to remove excess
stain. Each grid was then carefully dried using a vacuum hose. Imaging
was performed using a FEI Tecnai Spirit TEM instrument equipped with
a Gatan 1kMS600CW CCD camera operating at 120 kV.
3
Transmission Electron Microscopy of Diblock Copolymer Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Diblock copolymer
dispersions were diluted 50-fold to 0.20% w/w at 20 °C (for G54-H140 and G71-H200) or 50
°C (for G37-H80) prior to staining. Copper/palladium
TEM grids (Agar Scientific, UK) were surface-coated in-house to yield
a thin film of amorphous carbon. The grids were then treated with
a plasma glow discharge for 45 s to create a hydrophilic surface.
One 12 μL droplet of each aqueous copolymer dispersion was then
placed onto a freshly treated grid for 30 s and then blotted with
filter paper to remove excess solution. To stain the deposited nanoparticles,
a 0.75% w/w aqueous solution of uranyl formate (9 μL) was placed
via micropipet on the sample-loaded grid for 20 s and then carefully
blotted to remove excess stain. Each grid was then carefully dried
using a vacuum hose. Imaging was performed using a FEI Tecnai Spirit
TEM instrument equipped with a Gatan 1kMS600CW CCD camera and operating
at 80 kV.
dispersions were diluted 50-fold to 0.20% w/w at 20 °C (for G54-H140 and G71-H200) or 50
°C (for G37-H80) prior to staining. Copper/palladium
TEM grids (Agar Scientific, UK) were surface-coated in-house to yield
a thin film of amorphous carbon. The grids were then treated with
a plasma glow discharge for 45 s to create a hydrophilic surface.
One 12 μL droplet of each aqueous copolymer dispersion was then
placed onto a freshly treated grid for 30 s and then blotted with
filter paper to remove excess solution. To stain the deposited nanoparticles,
a 0.75% w/w aqueous solution of uranyl formate (9 μL) was placed
via micropipet on the sample-loaded grid for 20 s and then carefully
blotted to remove excess stain. Each grid was then carefully dried
using a vacuum hose. Imaging was performed using a FEI Tecnai Spirit
TEM instrument equipped with a Gatan 1kMS600CW CCD camera and operating
at 80 kV.
4
Copolymer Nanoparticle Visualization via TEM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Copolymer dispersions
were diluted fifty-fold at 20 °C to generate 0.20% w/w dispersions.
Copper/palladium TEM grids (Agar Scientific, UK) were coated in-house
to produce a thin film of amorphous carbon. These grids were then
treated with a plasma glow discharge for 30 s to create a hydrophilic
surface. Each aqueous diblock copolymer dispersion (12 μL; 0.20%
w/w) was placed on a freshly treated grid for 1 min and then blotted
with filter paper to remove excess solution. To stain the deposited
nanoparticles, an aqueous solution of uranyl formate (9 μL;
0.75% w/w) was placed on the sample-loaded grid via a micropipet for
20 s and then carefully blotted to remove excess stain. Each grid
was then carefully dried using a vacuum hose. Imaging was performed
using a FEI Tecnai Spirit TEM instrument equipped with a Gatan 1kMS600CW
CCD camera operating at 120 kV.
were diluted fifty-fold at 20 °C to generate 0.20% w/w dispersions.
Copper/palladium TEM grids (Agar Scientific, UK) were coated in-house
to produce a thin film of amorphous carbon. These grids were then
treated with a plasma glow discharge for 30 s to create a hydrophilic
surface. Each aqueous diblock copolymer dispersion (12 μL; 0.20%
w/w) was placed on a freshly treated grid for 1 min and then blotted
with filter paper to remove excess solution. To stain the deposited
nanoparticles, an aqueous solution of uranyl formate (9 μL;
0.75% w/w) was placed on the sample-loaded grid via a micropipet for
20 s and then carefully blotted to remove excess stain. Each grid
was then carefully dried using a vacuum hose. Imaging was performed
using a FEI Tecnai Spirit TEM instrument equipped with a Gatan 1kMS600CW
CCD camera operating at 120 kV.
5
Transmission Electron Microscopy of Polymer Vesicles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Aqueous copolymer dispersions were diluted at 20 °C to generate 0.10% w/w dispersions. Copper/palladium TEM grids (Agar Scientific, UK) were surface-coated in-house to yield a thin film of amorphous carbon. The grids were then plasma glow-discharged for 30 s to create a hydrophilic surface. One 12 μL droplet of each dispersion was adsorbed onto a freshly glow-discharged grid for 20 s and then blotted with filter paper to remove excess solution. To improve the contrast of the PGMA–PHPMA vesicles, uranyl formate (9 μL of a 0.75% w/v aqueous solution) was placed on each sample-loaded grid for 20 s and then carefully blotted to remove excess stain. To stain the PGMA–PHPMA–PDPA triblock copolymer aggregates, phosphotungstic acid (9 μL of a 1.0% w/v solution) was placed on each sample-loaded grid for 5 s and then carefully blotted to remove excess stain. Each grid was then dried using a vacuum hose. Imaging was performed using a FEI Tecnai Spirit TEM instrument equipped with a Gatan 1kMS600CW CCD camera operating at 120 kV.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!