Human embryonic kidney 293FT cells (Thermo Fisher Scientific) were cultured in high-glucose DMEM (Fujifilm Wako) supplemented with 10% fetal bovine serum (FBS). H3122 cells, which were gifted by JA Engelman (Massachusetts General Hospital Cancer Center, Boston, Massachusetts, USA), were cultured in RPMI-1640 medium (Wako Pure Chemical Industries) supplemented with 10% FBS and 100 μg/mL of kanamycin. HCC78 was obtained from DSMZ (Germany). HCC78xe3 ROS1-WT cell is a subclone of HCC78, generated by repeating subcutaneous implantation and in vitro cell culture 3 times, and induced SLC34A2-ROS1 overexpression as previously described (84 (link)). ALK fusion–positive and ROS1 fusion–positive NSCLC PDC lines were established from the patients’ pleural effusion. All patients provided informed consent for the genetic and cell biological analyses, which were performed in accordance with a protocol approved by the Institutional Review Board of the JFCR. NSCLC PDC lines JFCR-028-3 and JFCR-168 and HCC78 were cultured in RPMI and Ham’s F12 medium with 10 mM HEPES (Nacalai Tesque), 15% FBS, and 1× antibiotic-antimycotic mixed stock solution (Nacalai Tesque).
Hcc78
Manufactured by Leibniz Institute DSMZ
Sourced in Germany
The HCC78 is a piece of laboratory equipment used for cell culture applications. It is designed to provide a controlled environment for the growth and maintenance of cell lines. The core function of the HCC78 is to maintain temperature, humidity, and atmospheric conditions suitable for cell culture.
Automatically generated - may contain errors
Lab products found in correlation
Dmem high glucose, by Fujifilm (1 mentions)
Rpmi 1640 medium, by Fujifilm (1 mentions)
Antibiotic antimycotic mixed stock solution, by Nacalai Tesque (1 mentions)
Hepes, by Nacalai Tesque (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Matrigel, by Corning (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Iscove modified dulbecco media (imdm), by Thermo Fisher Scientific (1 mentions)
Mccoy s 5a, by Thermo Fisher Scientific (1 mentions)
Celltiter glo 3d cell viability assay kit, by Promega (1 mentions)
Fetal bovine serum (fbs), by ExCell Bio (1 mentions)
Dulbecco s pbs, by Corning (1 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
Leibovitz s l 15, by Merck Group (1 mentions)
Celltiter glo cell viability assay kit, by Promega (1 mentions)
Hybri care, by American Type Culture Collection (1 mentions)
H1975, by American Type Culture Collection (1 mentions)
H1299, by American Type Culture Collection (1 mentions)
3 protocols using hcc78
1
Culturing Human Lung Cancer Cell Lines
2
Cell Line Characterization Protocol
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Cancer cell lines were purchased from ATCC except for Huh7 (JCRB), PC-9 (Riken BRC), BEL7404 (SUZHOU BEILE BIOTECH), SK-OV-3, A2780, RPMI-8226 (ECACC), HCC78 (DSMZ) and KM-12 (HUATUO). Cell culture medium included RPMI-1640 (#22400-089), McCoy’s 5a (#16600082), DMEM (#11995-065), F-12K (#21127-022) and IMDM (#12440053) was purchased from Gibco. EMEM (#30-2003) and Hybri-Care (#46-X) medium was purchased from ATCC. Leibovitz’s L-15 (#L1518) was purchased from SIGMA. All the medium was contained 10% or 20% FBS (#FND500, ExCell Bio). The 2D and 3D models used the same culture medium. Horse serum (#041241A) was bought from BI. Trypsin (#25200072) and antibiotic-antimycotic (#15240-062) was purchased from Gibco. Dulbecco’s PBS (#21-031-CVC) and matrigel (#354234) were purchased from Corning. DMSO (#D2650) was bought from Sigma. CellTiter-Glo Cell Viability Assay kit (#G7573) and CellTiter-Glo 3D Cell Viability Assay kit (#G9683) were purchased from Promega.
3
Establishing Crizotinib-resistant Cell Lines
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HCC78 was purchased from the DSMZ. H3122 was kindly provided by Prof. Yong Peng (Sichuan University). PC-9 was purchased from the European Collection of Authenticated Cell Cultures (ECACC). Other cell lines including A549, H1299, HBE, HCC827 and H1975 cells were purchased from the American Type Culture Collection (ATCC). PC-9 and A549 were cultured in DMEM medium with 10% fetal bovine serum (FBS). Other cells were cultured in RPMI-1640 medium in the presence with 10% FBS fetal bovine serum. To establish the Crizotinib-resistant cells, HCC78 cells were maintained in complete medium with Crizotinib from a starting concentration of 100 nM to a final concentration of 1 μM over 6 months. The Crizotinib-resistant cells were maintained in complete medium with 0.5 μM Crizotinib. All cells were maintained under a humidified atmosphere of 5% CO2 at 37°C. Crizotinib (Sigma, #PZ0191), U0126 (Selleck, #S1102 chem), PD0325901 (Selleck, #S1036chem) and Cisplatin (Selleck, # S1166) were dissolved in dimethyl sulfoxide (DMSO) and stored at −20° or −80°C.
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