The tetrazolium-dye-based MTS assay was used to evaluate cell growth rate as described previously (Zheng et al., 2017 (link)). In brief, 1 × 104 cells per well were seeded in triplicate into 96-well plates for 24 h. Then, the cells were treated with different treatments according to design. After treatment, the cells were washed twice with PBS and treated with 2 mg/ml Hank’s buffer and incubated for 1 h. Then, the dark blue crystal should be seen under light microscopy. The crystals were dissolved in dimethyl sulfoxide, and the absorbance was measured with a Thermo Fluoroscan Ascent spectrometer at 570 nm.
Fluoroscan ascent spectrometer
Manufactured by Thermo Fisher Scientific
The Fluoroscan Ascent spectrometer is a fluorescence microplate reader designed for high-throughput screening, assay development, and quantitative analysis. It measures the fluorescence intensity of samples in microplates. The device utilizes multiple excitation and emission wavelengths to detect a wide range of fluorescent labels and dyes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fluoroscan ascent spectrometer
1
MTS Assay for Cell Viability
2
Cell Viability Evaluation by CCK-8
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cell viability was evaluated by CCK-8, as described previously [13] (link). In brief, 1 × 104 CFs/well were seeded in triplicate into 96-well plates and incubated overnight. After treatment, the CCK-8 working solution (10 µL) was added to the wells, and the plates were then incubated for 2 h at 37 °C. The absorbance was measured in a Thermo Fluoroscan Ascent spectrometer at 450 nm. Results were shown as the mean ± SEM of absorbance relative to time 0 or control.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!