Anti igg2b biotin

Manufactured by Southern Biotech

Anti-IgG2b-biotin is a laboratory reagent used as a detection antibody in various immunoassays. It binds to the IgG2b subclass of immunoglobulins and is conjugated with biotin, allowing for further detection and signal amplification.

Automatically generated - may contain errors

Lab products found in correlation

Anti igg1 biotin, by Southern Biotech (1 mentions) Streptavidin alkaline phosphatase, by Vector Laboratories (1 mentions) Salmon sperm dsdna, by Merck Group (1 mentions) Poly l lysine (pll), by Merck Group (1 mentions)

Close spelling variants of this product

Anti-IgG2b IgG2b

2 protocols using anti igg2b biotin

Measuring SARS-CoV-2 Antibody Titers

Check if the same lab product or an alternative is used in the 5 most similar protocols

IgG endpoint titers to mammalian-derived SARS-CoV-2 S, S1, S2 and RBD in sera at day 30 post-immunization were measured by Luminex immuno-assay. Assay was conducted as described above, with the modification of serially diluting serum 10-fold from 200 to 2 × 10⁸. Similarly, IgG subclass endpoint titers (i.e., IgG1 and IgG2a in BALB/c and IgG1 and IgG2c in C57BL/6) were measured against mammalian-derived SARS-CoV-2 S protein, using serially diluted mouse sera (5-fold from 200 to 3.1 × 10⁶) and secondary antibodies anti-IgG1-biotin, anti-IgG2a-biotin, or anti-IgG2b-biotin (SouthernBiotech). Four parameter logistic (4PL) curves were fitted to the measured MFI data from serially diluted sera, and three times the background (i.e., 3 × MFI with no serum) was used as a threshold cutoff to estimate endpoint titers.

de Alwis R., Gan E.S., Chen S., Leong Y.S., Tan H.C., Zhang S.L., Yau C., Low J.G., Kalimuddin S., Matsuda D., Allen E.C., Hartman P., Park K.J., Alayyoubi M., Bhaskaran H., Dukanovic A., Bao Y., Clemente B., Vega J., Roberts S., Gonzalez J.A., Sablad M., Yelin R., Taylor W., Tachikawa K., Parker S., Karmali P., Davis J., Sullivan B.M., Sullivan S.M., Hughes S.G., Chivukula P, & Ooi E.E. (2021). A single dose of self-transcribing and replicating RNA-based SARS-CoV-2 vaccine produces protective adaptive immunity in mice. Molecular Therapy, 29(6), 1970-1983.

+ Open protocol

+ Expand

ELISA for Anti-Nuclear Antibodies

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immulon 4HBX high binding plates (ThermoFisher, Rockford, IL) were used for ELISA assays. For ANA ELISAs, plates were coated with a 1:10 dilution of poly-L-lysine (Sigma-Aldrich, St. Louis, MO), followed by coating with salmon sperm dsDNA (Sigma-Aldrich, St. Louis, MO) or Smith/Ribonucleoprotein SmRNP (Arotec Diagnostics, Wellington, New Zealand). Plates were blocked with 4% non-fat dry milk in PBS and coated with diluted serum samples with serial double dilution. Detection of antibody subtypes was performed using the following combinations of primary and secondary detection antibodies: primary- anti-IgG-biotin (Jackson ImmunoResearch, West Grove, PA), anti-IgG2b-biotin (Southern Biotech, Birmingham, AL), anti-IgG2c-alkaline phosphatase (Southern Biotech, Birmingham, AL) and secondary- streptavidin-alkaline phosphatase (Vector Laboratories, Burlingame, CA). Plates were developed with PNPP (p-nitrophenyl phosphate, disodium salt) (ThermoFisher, Rockford, IL) substrate and quantitation was performed as previously described [50 (link)]. Anti-DNA or anti-SmRNP IgG-subclass titers were determined by calculating arbitrary units by using serial dilution of the serum from a positive animal as a standard, and were expressed as units per volume.

Soni C., Sinha I., Fasnacht M.J., Olsen N.J., Rahman Z.S, & Sinha R. (2019). Selenium supplementation suppresses immunological and serological features of lupus in B6.Sle1b mice. Autoimmunity, 52(2), 57-68.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!