Islet mass was measured on paraffin sections prepared as described [13 (link)] using an anti-chromogranin A antibody (Ab85554; Abcam, Toronto, ON, Canada) to label endocrine cells. Beta cell proliferation was measured by immunohistochemical staining of pancreas cryosections for Ki67 (Ab66155; Abcam) and insulin as described [13 (link)] or by intravenous injection of BrdU (B5002; Sigma-Aldrich, Oakville, ON, Canada) 50 mg/kg at 12, 36 and 60 h during the infusion followed by immunostaining for BrdU (347580; Becton Dickinson, San Jose, CA, USA) and insulin. Beta cell proliferation was expressed as a percentage of double-positive Ki67+ insulin+ (or BrdU+ insulin+) cells to total insulin+ cells. Apoptosis was measured using the FragEL DNA Fragmentation Detection Kit (Millipore, Etobicoke, ON, Canada) according to the manufacturer’s instructions (see ESM Methods ). The experimenter was blind to group assignments.
Ab85554
Manufactured by Abcam
Sourced in Canada
Ab85554 is a laboratory equipment product manufactured by Abcam. It is a specialized tool designed for use in scientific research and analysis. The core function of this product is to facilitate a specific laboratory process or measurement. No further details about the intended use or application of this product can be provided in an unbiased and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
Ab66155, by Abcam (1 mentions)
Fragel dna fragmentation detection kit, by Merck Group (1 mentions)
B5002, by Merck Group (1 mentions)
Ab34771, by Abcam (1 mentions)
A0099, by Agilent Technologies (1 mentions)
Ab13970, by Abcam (1 mentions)
Bz x700, by Keyence (1 mentions)
Fv1000d, by Olympus (1 mentions)
2 protocols using ab85554
1
Islet Morphometry and Beta Cell Proliferation
2
Comprehensive Intestinal Histology Analysis
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For pathological analysis, samples of the small intestine were fixed in 4% paraformaldehyde, embedded in paraffin, sectioned, and stained with H&E or Alcian blue. Immunofluorescence analysis was performed using primary antibodies (Abs), including rabbit antilysozyme (A0099; Dako), rat anti-Crp1 (77-R63), rabbit anti–MMP-7 (D4H5; Cell Signaling Technology), chicken anti-EGFP (ab13970; Abcam), rabbit anti-RFP (ab34771; Abcam), and rabbit anti–chromogranin A (ab85554; Abcam), and visualized with Alexa Fluor 488–, 555–, and 647–conjugated secondary Abs. Pictures of tissue sections were taken at room temperature using a digital camera (DP20; Olympus) mounted on a microscope (BX50; Olympus), fluorescence microscope (BZ-X700; Keyence), and confocal laser microscope (FV-1000D; Olympus).
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