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2 protocols using hydrocodone d6

1

Quantification of Opioids and Cannabinoids in Wastewater

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Morphine, oxycodone, hydrocodone, oxymorphone, hydromorphone, Δ9-tetrahydrocannabinol (THC) and its metabolites 11-nor-9-carboxy-tetrahydrocannabinol (THCCOOH) and THCCOOH-glucuronide were purchased from Cerilliant (Round Rock, TX, USA). The deuterated analogs THC-d3, THCCOOH-d3, THCCOOH-glucuronide-d3, Morphine-d3, oxycodone-d6, hydrocodone-d6, oxymorphone-d3 and hydromorphone-d6 were also purchased from Cerilliant. Strata XC 33 µm polymeric strong cation exchange cartridges of 3 mL/60 mg for calibrators and 6 mL/200 mg for quality control (QC) and authentic wastewater samples were purchased from Phenomenex (Torrance, CA, USA). Ultra-high performance liquid chromatography (UHPLC) grade methanol, dichloromethane and ammonium hydroxide were purchased from Pharmco-Aaper (Brookfield, CT). Isopropanol, liquid chromatography mass spectrometry grade acetonitrile, and Whatman glass microfiber filters (outside diameter 4.7 cm, particle retention 1.6 μm, and thickness 0.26 mm) were from Thermo Fisher Scientific (Waltham, MA, USA).
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2

Quantitative Liquid Chromatography-Mass Spectrometry Analysis

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Methanol, water, acetic acid and formic acid ULC/MS grade were from Biosolve (Valkenswaard, The Netherlands). Ethanol HPLC grade was purchased from Honeywell Riedel‐de Haën (Seelze, Germany). Dextromethorphan in Methanolic solution (1 mg·ml−1) was provided from Lipomed AG (Arlesheim, Switzerland). Dextrorphan tartrate, hydrocodone‐D6 and phenobarbital‐D5 in Methanolic solutions (1 mg·ml−1) were purchased from Cerilliant Corporation (Round Rock, USA). Ammonium acetate, potassium phosphate dibasic, potassium phosphate monobasic, nicotinamide adenine dinucleotide phosphate (NADP), isocitric acid, magnesium chloride hexahydrate and isocitrate dehydrogenase were purchased from Sigma‐Aldrich (Buchs, Switzerland).
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