Anti human mac 1 antibody

Pam3CSK4 was purchased from InvivoGen Co. (San Diego, CA). The anti-human Mac-1 antibody (cat no. 16-0113) and anti-mouse IgG isotype control antibody (cat no. 16-4714) were purchased from eBioscience (San Diego, CA). The R-phycoerythrin (PE)-conjugated mouse anti-human Mac-1 (clone ICRF44; BD) antibody (cat no. 555388) and PE-conjugated mouse IgG isotype control (clone MOPC-21) antibody (cat no. 555749) were obtained from BD (San Diego, CA). Calcein AM (an acetomethoxy (AM) derivative of calcein) and luciferase reporter constructs containing NF-κB (cat no. 219078) and CREB (cat no. 219076) consensus-binding sites were purchased from Agilent Technologies (Santa Clara, CA, USA). The anti-SIRT1 antibody and horseradish peroxidase (HRP)-conjugated IgG (secondary antibody) were obtained from Santa Cruz Biotechnology Inc. (Beverly, MA). ImProm-II Reverse Transcription Kits were supplied by the Promega Corporation (Madison, WI). DNeasy Tissue Kits and QIAprep Spin Kits were purchased from Qiagen (GmbH, Germany).

KLA (Kdo-Lipid A) was purchased from Adipogen (San Diego, CA, USA). Purified anti-human TLR4 antibody, anti-human Mac-1 antibody and anti-mouse IgG isotype control antibody were purchased from eBioscience (San Diego, CA). R-phycoerythrin (PE)-conjugated mouse anti-human Mac-1 (clone ICRF44; BD) antibody and PE-conjugated mouse IgG isotype control (clone MOPC-21) antibody were obtained from BD (San Diego, CA). Rabbit anti-mouse Mac-2 antibody was purchased from Abcam (Cambridge UK). MK-886 and various signal pathway inhibitors were purchased from EMD Serono, Inc. (Rockland, MA, USA) and Sigma-Aldrich Co. (Saint Louis, MO).