Miapaca 2

The human PDAC cell lines Capan-1 and MIAPaCa-2 and the embryonic kidney cell line HEK293T were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). NHDFs were purchased from TAKARA BIO (Shiga, Japan). The PCI43P5 cell line was previously established from surgically resected PDAC tissues at our institute [32 (link)]. All cell lines were cultured in the appropriate medium as directed by the manufacturers or according to published studies. Capan-1 was cultured in IMDM (WAKO, Tokyo, Japan) with 20% fetal bovine serum (Cell Culture Bioscience), and 1% penicillin/streptomycin (Life Technologies), and MIAPaCa-2, PCI43P5, NHDF, and HEK293T cells were cultured in RPMI 1640 (WAKO) with 10% fetal bovine serum, and 1% penicillin/streptomycin. All cells were incubated at 37°C in a mixture of 5% CO₂ and 95% air.

The human epidermoid carcinoma cell line A431, the human pancreatic cancer cell line MIAPaCa-2, and the mouse breast carcinoma cell line 4T1 were obtained from ATCC (Manassas, VA, USA). The 634NOD cell line was originally established from a pancreatic carcinoma in an HRAS^G12V transgenic rat [36 (link)]. A431 and MIAPaCa-2 cells were cultured in Dulbecco modified Eagle medium (Wako Pure Chemical Industries, Tokyo, Japan) containing 10% fetal bovine serum (FBS) (Life Technologies, Carlsbad, CA, USA) in 5% CO₂ at 37 °C. 4T1 cells were cultured in RPMI-1640 (Wako Pure Chemical Industries) containing 10% FBS in 5% CO₂ at 37 °C. 634NOD cells were cultured in RPMI-1640 containing 10% FBS, BEGM SingleQuots endothelial cell growth medium (Lonza, Basel, Switzerland), and antibiotic antimycotic solution (GE Healthcare, Little Chalfont, UK) in 5% CO₂ at 37 °C.

The human pancreatic cancer cell lines PANC-1 and MIA PaCa-2 were purchased from the American Type Culture Collection (ATCC, Rockville, MD). PANC-1 and MIA PaCa-2 were guaranteed by ATCC, and maintained in Dulbecco’s modified Eagle’s Medium (D-MEM) (Wako Pure Chemical Industries, Ltd., Osaka, Japan) containing 10% fetal bovine serum (Gibco, Grand Island, NY) and 1% penicillin/streptomycin (Gibco/Brl). The cells were cultured at 37° C with 5% CO₂.