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Ab31630

Manufactured by MP Biomedicals

Ab31630 is a laboratory instrument produced by MP Biomedicals. It is designed for scientific research and analysis purposes. The core function of this product is to perform specific tasks related to the handling and processing of samples in a laboratory setting.

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2 protocols using ab31630

1

Immunohistochemistry Analysis of Neuronal Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry analyses were performed on whole mount fly larvae, cultured neurons and on mouse tissue sections collected from OCT-embedded tissues. Primary antibodies used were anti-Tuj1 (Covance, MMS-435P, 1:1,000), anti-Tau (Millipore, MAB3420, 1:1,000), anti-RTCA (Sigma, SAB2102059 and HPA027982, 1:200), anti-CTIP2 (Abcam, ab18465, 1:2,000), anti-NF200 (Sigma, N4142, 1:200), anti-ED1 (Abcam, ab31630, 1:200), anti-β-Galactosidase (MP Biomedicals cappel, 55976, 1:5,000) and anti-NeuN (Millipore, MAB377, 1:200). A custom-made antibody against the entire dRtca protein was generated (Thermo, 1:200). LacZ staining was performed using the Senescence β-Galactosidase Staining Kit (Cell signaling) according to manufacturer’s instructions.
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2

Immunohistochemistry Analysis of Neuronal Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry analyses were performed on whole mount fly larvae, cultured neurons and on mouse tissue sections collected from OCT-embedded tissues. Primary antibodies used were anti-Tuj1 (Covance, MMS-435P, 1:1,000), anti-Tau (Millipore, MAB3420, 1:1,000), anti-RTCA (Sigma, SAB2102059 and HPA027982, 1:200), anti-CTIP2 (Abcam, ab18465, 1:2,000), anti-NF200 (Sigma, N4142, 1:200), anti-ED1 (Abcam, ab31630, 1:200), anti-β-Galactosidase (MP Biomedicals cappel, 55976, 1:5,000) and anti-NeuN (Millipore, MAB377, 1:200). A custom-made antibody against the entire dRtca protein was generated (Thermo, 1:200). LacZ staining was performed using the Senescence β-Galactosidase Staining Kit (Cell signaling) according to manufacturer’s instructions.
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