As previously reported44 ,92 (link), venous blood samples were drawn into 9 ml heparin coated plasma tubes after 12 h overnight fasting and were centrifuged for 10 min at 2000 × g within 1 h from blood collection. Plasma samples were then transferred into 0.5 ml polypropylene microtubes and stored in a freezer at − 80 °C. Circulating levels of high-sensitivity C-Reactive Protein (CRP) in mg/dL were assayed using a human CRP Quantikine enzyme-linked immunosorbent assay (ELISA) kit (R & D Systems, Minneapolis, USA). Six individuals had a CRP value > 10 mg/ml indicative of acute infection and were, therefore, excluded from the statistical analyses testing for moderating effects of inflammation. Leptin concentrations in pg/ml were determined with the DRP300 Quantikine ELISA kit (R & D Systems) and adiponectin in ng/ml with the human total adiponectin/Acrp30 Quantitkine ELISA kit (R & D Systems). Leptin/adiponectin ratios for each participant were calculated. Interleukin IL-8 levels in pg/mL were determined using a high sensitivity CXCL8/ INTERLEUKIN-8 Quantikine ELISA kit (R & D Systems). Determination of interleukin-1β, interleukin-6 and Tumor Necrosis Factor α (TNFα) levels were trialled with high-sensitivity Quantikine ELISA kits but did not result in reliable measurements consistently above the level of detection for each assay.
Cxcl8 interleukin 8 quantikine elisa kit
Manufactured by R&D Systems
The CXCL8/ INTERLEUKIN-8 Quantikine ELISA kit is a quantitative sandwich enzyme immunoassay designed for the measurement of CXCL8/Interleukin-8 levels in cell culture supernates, serum, and plasma.
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3 protocols using cxcl8 interleukin 8 quantikine elisa kit
1
Plasma Biomarkers of Inflammation and Metabolism
2
Biomarkers of Inflammation and Metabolic Health
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As previously reported (Metzler-Baddeley et al., 2019a) , venous blood samples were drawn into 9ml heparin coated plasma tubes after 12 hours overnight fasting and were centrifuged for 10 minutes at 2,000xg within 1 hour from blood collection. Plasma samples were then transferred into 0.5 ml polypropylene microtubes and stored in a freezer at -80°C.
Circulating levels of high-sensitivity C-Reactive Protein (CRP) in mg/dL were assayed using a human CRP Quantikine enzyme-linked immunosorbent assay (ELISA) kit (R & D Systems, Minneapolis, USA). Six individuals had a CRP value > 10 mg/ml indicative of acute infection and were therefore excluded from analyses testing for moderating effects of inflammation.
Leptin concentrations in pg/ml were determined with the DRP300 Quantikine ELISA kit (R & D Systems) and adiponectin in ng/ml with the human total adiponectin/Acrp30 Quantitkine ELISA kit (R & D Systems). Leptin/adiponectin ratios for each participant were calculated.
Interleukin IL-8 levels in pg/mL were determined using a high sensitivity CXCL8/ INTERLEUKIN-8 Quantikine ELISA kit (R & D Systems). Determination of interleukin-1β, interleukin-6 and Tumor Necrosis Factor α (TNFα) levels were trialled with high-sensitivity Quantikine ELISA kits but did not result in reliable measurements consistently above the level of detection for each assay.
Circulating levels of high-sensitivity C-Reactive Protein (CRP) in mg/dL were assayed using a human CRP Quantikine enzyme-linked immunosorbent assay (ELISA) kit (R & D Systems, Minneapolis, USA). Six individuals had a CRP value > 10 mg/ml indicative of acute infection and were therefore excluded from analyses testing for moderating effects of inflammation.
Leptin concentrations in pg/ml were determined with the DRP300 Quantikine ELISA kit (R & D Systems) and adiponectin in ng/ml with the human total adiponectin/Acrp30 Quantitkine ELISA kit (R & D Systems). Leptin/adiponectin ratios for each participant were calculated.
Interleukin IL-8 levels in pg/mL were determined using a high sensitivity CXCL8/ INTERLEUKIN-8 Quantikine ELISA kit (R & D Systems). Determination of interleukin-1β, interleukin-6 and Tumor Necrosis Factor α (TNFα) levels were trialled with high-sensitivity Quantikine ELISA kits but did not result in reliable measurements consistently above the level of detection for each assay.
3
Biomarker Measurement Protocol for Chronic Disease
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Venous fasting blood samples were drawn into 9 ml heparin coated plasma tubes after 12 h overnight fasting and were centrifuged for 10 min at 2,000×g within 60 min from blood collection. Plasma samples were transferred into 0.5 ml polypropylene microtubes and stored in a freezer at −80 °C.
Circulating levels of high-sensitivity CRP in mg/dL were assayed using a human CRP Quantikine enzyme-linked immunosorbent assay (ELISA) kit (R & D Systems) and IL-8 levels in pg/mL were determined using a high sensitivity CXCL8/INTERLEUKIN-8 Quantikine ELISA kit (R & D Systems). Leptin concentrations in pg/ml were determined with the DRP300 Quantikine ELISA kit (R & D Systems) and adiponectin in ng/ml with the human total adiponectin/Acrp30 Quantitkine ELISA kit (R & D Systems) and leptin/adiponectin ratios for each participant were calculated (Lopez-Jaramillo et al., 2014 ). Determination of interleukin-1β, interleukin-6 and Tumor Necrosis Factor α (TNFα) levels were trialed with Quantikine ELISA kits but did not lead to reliable measurements consistently above the level of detection for each assay. All ELISA analyses were carried out in the laboratory of the School of Pharmacy and Pharmaceutical Sciences at Cardiff University.
Circulating levels of high-sensitivity CRP in mg/dL were assayed using a human CRP Quantikine enzyme-linked immunosorbent assay (ELISA) kit (R & D Systems) and IL-8 levels in pg/mL were determined using a high sensitivity CXCL8/INTERLEUKIN-8 Quantikine ELISA kit (R & D Systems). Leptin concentrations in pg/ml were determined with the DRP300 Quantikine ELISA kit (R & D Systems) and adiponectin in ng/ml with the human total adiponectin/Acrp30 Quantitkine ELISA kit (R & D Systems) and leptin/adiponectin ratios for each participant were calculated (Lopez-Jaramillo et al., 2014 ). Determination of interleukin-1β, interleukin-6 and Tumor Necrosis Factor α (TNFα) levels were trialed with Quantikine ELISA kits but did not lead to reliable measurements consistently above the level of detection for each assay. All ELISA analyses were carried out in the laboratory of the School of Pharmacy and Pharmaceutical Sciences at Cardiff University.
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