Goat anti rabbit and goat anti mouse horseradish peroxidase hrp conjugated secondary antibodies

Neurobasal medium, B27 supplement, high-glucose Dulbecco’s Modified Eagle Medium (DMEM), and fetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA). pcDNA3.0, pcDNA-CDK5, and GFP-p25 plasmids were obtained from Addgene (Cambridge, MA, USA). An NF-κB luciferase reporter plasmid was purchased from Beyotime Institute of Biotechnology (Shanghai, China). Lipofectamine 2000 transfection reagent and Opti-MEMI medium were obtained from Invitrogen (Carlsbad, CA, USA). Etoposide and roscovitine were obtained from Sigma-Aldrich (St. Louis, MO, USA). A luciferase reporter gene assay kit was purchased from Roche (Basel, Switzerland). The 96-well plate used for the luciferase reporter gene test was purchased from Greiner (Lud-wigsburg, Germany). Other cell culture plates were purchased from Corning (Corning, NY, USA). The primary antibody against caspase 1 was obtained from Abcam (Cambridge, MA, USA). Primary antibodies, including anti-CDK5, anti-phosphorylated (p)-CDK5, anti-IL-1β, and anti-β-actin antibodies, were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Goat anti-rabbit and goat-anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Jackson Immuno Research (West Grove, PA, USA).