Blood smears of 192 individual C. livia were fixed with methanol and stained with Giemsa stain following the standard protocol of Hemacolor® Rapid staining of blood smear kit (Merck, Darmstadt, Germany). The slides were first scanned under the microscope at × 400 magnification to determine the presence or absence of haemosporidian blood parasites. For infected individuals, the intensity of infection was then determined by scanning each slide at × 1000 magnification with an oil immersion lens and counting the number of parasites seen within 10,000 erythrocytes.
Hemacolor rapid staining of blood smear kit
Manufactured by Merck Group
Sourced in Germany
Hemacolor Rapid staining of blood smear kit is a laboratory product designed to enable the rapid staining of blood smear samples. It provides a standardized and efficient method for the preparation of blood samples for microscopic examination.
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3 protocols using hemacolor rapid staining of blood smear kit
1
Giemsa Staining of Avian Blood Smears
2
Blood Smear Analysis and Serum Biomarkers
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Blood smears were stained using the May–Grünwald–Giemsa stain (Hemacolor Rapid staining of blood smear kit; Merck KGaA, Darmstadt, Germany) [4 (link)], following which 100 leukocytes, including granular (heterophils, eosinophils, and basophils) and nongranular (lymphocytes and monocytes), were counted per slide using light microscopy (Leitz Orthoplan, Leitz, Wetzlar, Germany) at 100-times magnification. The heterophil:lymphocyte ratio was calculated [10 (link)]. Serum glucose, uric acid, triglycerides, cholesterol, and non-esterified fatty acids (NEFA) were determined using standard enzymatic colorimetric analysis using an autoanalyzer for clinical chemistry (Cobas 6000/c501; Roche Diagnostics GmbH, Vienna, Austria).
3
Hematological and Biochemical Profiling of Chickens
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Blood smears were stained using the May-Grünwald-Giemsa stain (Hemacolor Rapid staining of blood smear kit; Merck KGaA, Darmstadt, Germany). A total of 100 leukocytes, including granular (heterophils, eosinophils, and basophils) and nongranular (lymphocytes and monocytes), were counted per slide using light microscopy (Leitz Orthoplan, Leitz, Wetzlar, Germany) at 100-times magnification, and the H-to-L ratio was calculated (Gross and Siegel, 1983) . Serum glucose, uric acid, triglycerides, cholesterol and NEFA were determined by standard enzymatic colorimetric analysis using an autoanalyzer for clinical chemistry (Cobas 6000/c501; Roche Diagnostics GmbH, Vienna, Austria). Chicken specific commercial ELISA kits were used to determine the APPs ovotransferrin (OVT; Cusabio, Wuhan, China) and alpha-1-acid glycoprotein (AGP; Genway Biotech Inc., San Diego, CA, US) in serum according to the manufacturers' instructions. Samples were diluted 2 to 5-fold for both assays depending on the individual sample concentration. The intra-and interassay variability for the OVT and AGP kits were less than 10%, respectively, and the detection limit was 0.039 ng/ml and 3.125 ng/ml. All serum parameters were analyzed together at L1.
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