The ink injected skin samples were cut out and fixed with 4% paraformaldehyde, embedded in polyester wax (VWR International), and sectioned for hematoxylin and eosin (H&E) staining observation. To detect Langerin+ cells, the back skin was removed at 24 hours after DT injection, and frozen skin specimens were sectioned and fixed by microwave irradiation and methanol. After blockade with 3% BSA, 2% skim milk, 0.1% Triton X100 in PBS, slides were stained with Langerin (Rabbit IgG poly; IMGENEX) antibody in 2% skim milk, 0.1% Triton X100 in PBS, followed by incubation with FITC-conjugated swine anti-rabbit Igs (DAKO) secondary antibody. Slides were counterstained with DAPI (Vector Laboratories).
Polyester wax
Manufactured by Avantor
Polyester wax is a type of solid wax material commonly used in various laboratory applications. It is a synthetic polymer-based wax that offers specific physical and chemical properties. The core function of polyester wax is to provide a stable and consistent medium for various laboratory processes and procedures.
Automatically generated - may contain errors
2 protocols using polyester wax
1
Langerin+ Cells Detection in Skin
2
Tissue Sectioning and Mounting Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Samples were infiltrated and embedded in polyester wax (VWR) [9 (link)], and then sliced into serial sections (5 μm thickness) using a rotary microtome RV-240 (Yamato Koki). The sections were mounted on silane-coated Platinum Pro glass slides (Matsunami Glass), and dewaxed in 100% ethanol. The samples were then rehydrated using a graded ethanol to phosphate-buffered saline (PBS) series in descending concentrations.
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