Crl skh1 hrhr

Manufactured by Charles River Laboratories

Sourced in Germany

The Crl:SKH1-Hrhr is a hairless rat strain developed by Charles River Laboratories. It is a well-established animal model used in various research applications. The strain is characterized by its lack of fur, which is caused by a genetic mutation.

Automatically generated - may contain errors

Lab products found in correlation

Nih 3 nude mice crl nih lystbg jfoxn1nubtkxid, by Charles River Laboratories (2 mentions) C57bl 6 mice, by Taconic Biosciences (2 mentions)

7 protocols using crl skh1 hrhr

Genetic Manipulation of Mif and Ddt Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ddt^−/−, Mif^−/− and Mif^−/−/Ddt^−/− mice were recently described^{28 (link)}. All mice were outbred to the SKH-1 background (Crl:SKH1-Hrhr; Charles River, Sulzfeld, Germany) for 10 generations. For all studies, 8–10 week old male mice were used. At the end of each experiment, mice were euthanized by cervical dislocation. Experimental procedures were approved by the administration of the North Rhine-Westphalian Agency for Nature, Environment and Consumer Protection (Landesamt für Umwelt, Natur und Verbraucherschutz—LANUV, Recklinghausen, Germany—AZ 84-02.04. 2017.A303) and were in accordance with ARRIVE guidelines and the Federation of European Laboratory Animal Science Associations/Society of Laboratory Animal Science (FELASA/GV-SOLAS) Guidelines.

Huth S., Huth L., Heise R., Marquardt Y., Lopopolo L., Piecychna M., Boor P., Fingerle-Rowson G., Kapurniotu A., Yazdi A.S., Bucala R., Bernhagen J, & Baron J.M. (2023). Macrophage migration inhibitory factor (MIF) and its homolog D-dopachrome tautomerase (D-DT) are significant promotors of UVB- but not chemically induced non-melanoma skin cancer. Scientific Reports, 13, 11611.

+ Open protocol

+ Expand

In Vivo Luciferase mRNA Imaging in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Female hairless mice of strain Crl:SKH1-Hr^hr (Charles River Laboratories) of an age of 7 weeks were used for in vivo imaging. Studies were conducted by an external CRO (Preclinics GmbH, Potsdam, Germany). First, the mice were put under infrared light for vasodilation. 30 µg of Viromer-complexed FLuc mRNA imaging, the tail vein was punctured using a 27G cannula for luciferin administration. Injection volume was 10 mL/kg containing 15 mg/mL luciferin. Afterwards the animals were anesthetized isoflurane inhalation. The animals were imaged from the ventral side for 300 s under continuous isoflurane anaesthesia. Then the animals were turned and imaged again for 300 s from the lateral side. Expression of luciferase was monitored after 6 and 24 h using a luminescence CCD camera (Andor Technology Ltd). Afterwards, animals were allowed to wake up.

Jansig E., Geissler S., Rieckmann V., Kuenemund A., Hietel B., Schenk M., Wussow S., Kreideweiss P., Panzner S., Reinsch C, & Cynis H. (2020). Viromers as carriers for mRNA-mediated expression of therapeutic molecules under inflammatory conditions. Scientific Reports, 10, 15090.

+ Open protocol

+ Expand

Measuring Circadian Rhythms in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

To measure peripheral PER2-bioluminescence levels, we made use of the Per2^Luc KI construct (Yoo et al., 2004 (link)). The KI construct was originally generated on a C57BL/6J-129 mixed background and subsequently brought onto a C57BL/6J (B6) background by backcrossing for at least 11 generations. These mice were then crossed with outbred SKH1 mice (Crl:SKH1-Hrhr; Charles River) to create hairless Per2^Luc KI mice. We used male homozygous Per2^Luc KI B6 and hairless heterozygous Per2^Luc KI SKH1-B6 hybrid (here referred to as SKH1 mice) mice. Mice were kept under a 12 hr light/12 hr dark cycle with light- and dark onset referred to as Zeitgeber time (ZT)-0 and -12, respectively. Age at time of recording varied between 12 and 24 weeks. Food and water was available ad libitum, and after surgery mice were singly housed. All experiments were approved by the Ethical Committee of the State of Vaud Veterinary Office Switzerland under license VD2743, 3201, and 3402.

Hoekstra M.M., Jan M., Katsioudi G., Emmenegger Y, & Franken P. (2021). The sleep-wake distribution contributes to the peripheral rhythms in PERIOD-2. eLife, 10, e69773.

+ Open protocol

+ Expand

Breeding B6.albino; Hairless Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

The B6.albino; hairless mice B6.Cg-Tyr^c-2J Hr^hr/J, (JAX stock # 017840) was constructed at The Jackson Laboratory by mating C57BL/6J-albino mice (Stock No. 000058) with C57BL/6-hairless mice (also segregating for H2-T18^a; Stock No. 001737). The B6.albino; hairless colony was then maintained by breeding females homozygous for the albino mutation and heterozygous for the hairless mutation with males homozygous for the albino mutation and homozygous for the hairless mutation. During the first few rounds of breeding, animals were screened and selected to replace the H2-T18^a allele with the respective C57BL/6J allele (H2-T18^b). Hairless, albino SKH1 mice were purchased from Charles Rivers Laboratories (Crl:SKH1-Hr^hr; Wilmington, MA; referred to as SKH1 mice throughout the manuscript). While SKH1 mice do not exhibit breeding issues, female B6.Cg-Tyr^c-2J Hr^hr/J mice homozygous for the mutated Hr allele are fertile, but exhibit poor nursing behavior resulting in reduced or absent litter. Similar breeding issues have been described for HRS/J-Hr^hr/hr mice [40 (link)], the strain from which the Hr^hr allele was obtained from to create the B6.Cg-Hr^hr/J congenic strain. All mouse studies were approved by the Indiana University-Purdue University at Indianapolis Animal Care and Use Committee.

Konger R.L., Derr-Yellin E., Hojati D., Lutz C, & Sundberg J.P. (2016). Comparison of the acute ultraviolet photoresponse in congenic albino hairless C57BL/6J mice relative to outbred SKH1 hairless mice. Experimental dermatology, 25(9), 688-693.

+ Open protocol

+ Expand

Animal Welfare in Rodent Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

Compliance with ethical regulations and all animal experiments were approved by University Committee on Animal Resources of University of Rochester. The blind mole rats (BMRs, Spalax galili) were wild caught from Upper Eastern Galilee Mountains, Israel, and were housed individually. C57BL/6 mice of both sexes were purchased from Taconic Biosciences, Inc. NIH III Nude mice (Crl:NIH-Lyst^bg-JFoxn1^nuBtk^xid) and SKH1 hairless mice (Crl:SKH1-Hrhr) were purchased from Charles river Laboratories Inc.

Zhao Y., Oreskovic E., Zhang Q., Lu Q., Gilman A., Lin Y.S., He J., Zheng Z., Lu J.Y., Lee J., Ke Z., Ablaeva J., Sweet M., Horvath S., Zhang Z., Nevo E., Seluanov A, & Gorbunova V. (2021). Transposon-triggered innate immune response confers cancer resistance to the blind mole rat. Nature immunology, 22(10), 1219-1230.

+ Open protocol

+ Expand

Animal Welfare in Rodent Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

Measuring Circadian PER2 Bioluminescence

Check if the same lab product or an alternative is used in the 5 most similar protocols

To measure peripheral PER2 bioluminescence levels we made use of the Per2::Luc KI construct (Yoo et al., 2004) (link). The Per2::Luc KI construct was originally generated on a C57BL/6J-129 mixed background and subsequently brought onto a C57BL/6J (B6)
background by backcrossing for at least 11 generations. These mice where then crossed with outbred SKH1 mice (Crl:SKH1-Hrhr; Charles River) to create hairless Per2::Luc KI mice. We used male homozygous Per2::Luc KI B6 and hairless heterozygous Per2::Luc KI SKH1-B6
hybrid (here referred to as SKH1 mice) mice. Mice were kept under a 12 h-light/12 h-dark cycle with light-and dark-onset referred to as Zeitgeber time (ZT)-0 and -12, respectively. Age at time of recording varied between 12 and 24 weeks. Food and water was available ad libitum, and after surgery mice were singly housed. All experiments were approved by the Ethical Committee of the State of Vaud Veterinary Office Switzerland under license VD2743 and 3201.

Hoekstra M.M., M J., Emmenegger Y., & Franken P. (2020). The sleep-wake distribution contributes to the peripheral rhythms in PERIOD-2.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!