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Kraslsl g12d mice

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The KrasLSL-G12D mice are a genetically engineered mouse model that carries a conditional Kras G12D mutation. The Kras G12D mutation is a common oncogenic driver found in various types of cancer. These mice can be used to study the effects of this mutation in different cell types and disease models.

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Lab products found in correlation

Lgr5 egfp ires creert2 mice, by Jackson ImmunoResearch (1 mentions) Trp53flox flox, by Jackson ImmunoResearch (1 mentions) R26 pik3cah1047r, by Jackson ImmunoResearch (1 mentions) C57bl 6j strain, by CLEA Japan (1 mentions) Balb ca nu nu nude mice, by CLEA Japan (1 mentions) Trp53flox flox mice, by Jackson ImmunoResearch (1 mentions) C57bl 6j, by Jackson ImmunoResearch (1 mentions) Cd 1 mice, by Charles River Laboratories (1 mentions) Ptet on, by Takara Bio (1 mentions) Nod scid il2rγ null mice, by Charles River Laboratories (1 mentions) Rosa26 yfp mice, by Jackson ImmunoResearch (1 mentions) Trp53fl fl mice, by Jackson ImmunoResearch (1 mentions) Shh cre mice, by Jackson ImmunoResearch (1 mentions)

11 protocols using kraslsl g12d mice

1

Genetic Mouse Models in Research

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Villin-Cre mice (stock number: T000142) were purchased from the National Resource Center of Model Mice. Lgr5-EGFP-IRES-CreERT2 mice (stock number: 008875) and KrasLSL-G12D mice (stock number: 008179) were obtained from Jackson Laboratories. Mex3a KO mice were obtained from Xin Wu's laboratory at Nanjing Medical University. Mex3aflox/flox mice were generated at the Shanghai Model Organisms Center, and first exon (1328 bp) of Mex3a was targeted with flanking LoxP sites, resulting in two LoxP loci.
Yang X., Li G., Tian Y., Wang X., Xu J., Liu R., Deng M., Shao C., Pan Y., Wu X., Li M., Zhang C., Liu R., Qin J., Zhang C., Liu Z., Wu X., Plikus M.V., Lengner C.J., Zheng Z., Lv C, & Yu Z. (2022). Identifying the E2F3-MEX3A-KLF4 signaling axis that sustains cancer cells in undifferentiated and proliferative state. Theranostics, 12(16), 6865-6882.
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2

Conditional Kras Lung Cancer Model

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TetO_Lox-Stop-Lox-KrasG12D (tetO_LKrasG12D), p48-Cre, Rosa26-Lsl-rtTA-Ires-Gfp (Rosa_rtTA) and Trp53L strains were donated by Dr. DePinho and described previously27 (link),28 (link). KrasLSLG12D mice were obtained through the Jackson Laboratory. SDCL mice were a generous gift from Dr. Bernfield29 (link). Mice were interbred and maintained on FVB/C57Bl6 hybrid background in pathogen-free conditions at The University of Texas MD Anderson Cancer Center. Animals of both sexes are used in the experiments. Mice were fed with doxycycline water (doxycycline 2g/l, sucrose 20 g/l) where indicated. All manipulations were approved under Institutional Animal Care and Use Committee (IACUC) protocol 00001238. The maximal tumor size allowed by IACUC is 1.5cm in diameter.
Yao W., Rose J.L., Wang W., Seth S., Jiang H., Taguchi A., Liu J., Yan L., Kapoor A., Hou P., Chen Z., Wang Q., Nezi L., Xu Z., Yao J., Hu B., Pettazzoni P.F., Ho I.L., Feng N., Ramamoorthy V., Jiang S., Deng P., Ma G.J., Den P., Tan Z., Zhang S.X., Wang H., Wang A.Y., Deem A.K., Fleming J.B., Carugo A., Heffernan T.P., Maitra A., Viale A., Ying H., Hanash S., DePinho R.A, & Draetta G.F. (2019). Syndecan1 is a critical mediator of macropinocytosis in pancreatic cancer. Nature, 568(7752), 410-414.
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3

Conditional Kras Lung Cancer Model

Cited 2 times
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TetO_Lox-Stop-Lox-KrasG12D (tetO_LKrasG12D), p48-Cre, Rosa26-Lsl-rtTA-Ires-Gfp (Rosa_rtTA) and Trp53L strains were donated by Dr. DePinho and described previously27 (link),28 (link). KrasLSLG12D mice were obtained through the Jackson Laboratory. SDCL mice were a generous gift from Dr. Bernfield29 (link). Mice were interbred and maintained on FVB/C57Bl6 hybrid background in pathogen-free conditions at The University of Texas MD Anderson Cancer Center. Animals of both sexes are used in the experiments. Mice were fed with doxycycline water (doxycycline 2g/l, sucrose 20 g/l) where indicated. All manipulations were approved under Institutional Animal Care and Use Committee (IACUC) protocol 00001238. The maximal tumor size allowed by IACUC is 1.5cm in diameter.
Yao W., Rose J.L., Wang W., Seth S., Jiang H., Taguchi A., Liu J., Yan L., Kapoor A., Hou P., Chen Z., Wang Q., Nezi L., Xu Z., Yao J., Hu B., Pettazzoni P.F., Ho I.L., Feng N., Ramamoorthy V., Jiang S., Deng P., Ma G.J., Den P., Tan Z., Zhang S.X., Wang H., Wang A.Y., Deem A.K., Fleming J.B., Carugo A., Heffernan T.P., Maitra A., Viale A., Ying H., Hanash S., DePinho R.A, & Draetta G.F. (2019). Syndecan1 is a critical mediator of macropinocytosis in pancreatic cancer. Nature, 568(7752), 410-414.
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4

Generating Compound Mutant Mice

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The KrasLSL-G12D/+ mice, Trp53flox/flox, and R26-Pik3caH1047R mice were imported from The Jackson Laboratory (Bar Harbor, ME) and were maintained in a C57BL/6J background. These mice were intercrossed to generate compound mutant mice.
Kato S., Fushimi K., Yabuki Y., Maru Y., Hasegawa S., Matsuura T., Kurotaki D., Suzuki A., Kobayashi N., Yoneda M., Higurashi T., Enaka M., Tamura T., Hippo Y, & Nakajima A. (2021). Precision modeling of gall bladder cancer patients in mice based on orthotopic implantation of organoid-derived tumor buds. Oncogenesis, 10(4), 33.
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5

Transgenic Mouse Models for Kras-Driven Cancer

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PINCH-1fl/fl transgenic mice were generated in a previous study57 (link),58 (link). KrasLSL-G12D/+ mice were bought from the Jackson Laboratory. All mouse work was performed with the approval of the Institutional Animal Care and Use Committee, Southern University of Science and Technology.
Guo L., Cui C., Wang J., Yuan J., Yang Q., Zhang P., Su W., Bao R., Ran J, & Wu C. (2020). PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth. Nature Communications, 11, 4913.
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6

Generation of Genetically Engineered Mice

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Female mice of the C57BL/6 J strain and Balb/cAnu/nu (nude mice) at 5 weeks of age were purchased from CLEA Japan Inc. (Tokyo, Japan). Conditional knock-in mice heterozygous for the Lox-STOP-Lox-KrasG12D allele (hereafter referred to as KrasLSL-G12D/+ mice) and Trp53flox/flox mice were obtained from the Jackson Laboratory (Bar Harbor, ME, USA) and maintained in a C57BL/6 J background. These mice were intercrossed to generate KrasLSL-G12D/+; Trp53flox/flox mice. Genotyping was performed after weaning as previously described [28 (link),50 (link),]. Animal studies were carried out with the approval of the Chiba Cancer Center for Ethics in Animal Experimentation.
Maru Y., Tanaka N., Tatsumi Y., Nakamura Y., Itami M, & Hippo Y. (2021). Kras activation in endometrial organoids drives cellular transformation and epithelial-mesenchymal transition. Oncogenesis, 10(6), 46.
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7

Transgenic Embryonic Mouse Model

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The UBC and UCSF Animal Care Committees approved animal experiments. Mice were housed on laboratory chow diet on a 12-hour light-dark cycle. Noon on the morning of the discovery of a vaginal plug was considered embryonic day (E)0.5. CD-1 mice were obtained from Charles River. Frozen embryos of Pdx1-Cre (Gu et al., 2002 (link)) mice were obtained from the MMRRC and were rederived by the BC Preclinical Research Consortium (Vancouver BC, Canada). KrasLSL-G12D mice (Jackson et al., 2001 (link)) and C57BL/6(tetO-Cdkn1b)1Scpr/J mice (Pruitt et al., 2013 ) were purchased from Jackson Laboratory. Sox9-rtTA transgenic mice were made by recombining the reverse tetracycline transactivator (Clontech; pTet-On) into the Sox9 coding region of a 202kb BAC (RP23-36D5); transgenic mice were generated by the UCSF transgenic core as described (Shimajiri et al., 2011 ). Transgene expression was induced in pregnant Sox9-rtTA; tetO-Cdkn1b with daily 1 mg intraperitoneal (IP) injections of doxycycline on indicated gestational days.
Krentz N.A., van Hoof D., Li Z., Watanabe A., Tang M., Nian C., German M.S, & Lynn F.C. (2017). Phosphorylation of NEUROG3 Links Endocrine Differentiation to the Cell Cycle in Pancreatic Progenitors. Developmental cell, 41(2), 129-142.e6.
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8

Transgenic Mouse Models for Tumorigenesis

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Kindlin-2fl/fl transgenic mice were generated as described36 (link). KrasLSL−G12D/+ mice were bought from the Jackson Laboratory. All mouse work was performed with the approval of the Institutional Animal Care and Use Committee, Southern University of Science and Technology.
Guo L., Cui C., Zhang K., Wang J., Wang Y., Lu Y., Chen K., Yuan J., Xiao G., Tang B., Sun Y, & Wu C. (2019). Kindlin-2 links mechano-environment to proline synthesis and tumor growth. Nature Communications, 10, 845.
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9

Genetic Mouse Models for Cancer Research

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Rosa26-YFP mice42 (link), Lgr5-creER mice43 (link), KrasLSL−G12D mice44 (link) and Trp53fl/fl mice45 (link) were imported from the NCI mouse repository and Jackson Laboratories. NOD/SCID/Il2Rγ null mice were purchased from Charles River. All mouse groups used in this study were composed of males and females with mixed genetic background. No randomization and no blinding were performed in this study. The Rosa26LSL-NTN1 transgenic mice (for LKPR-NTN1 gain of function) were imported from Mehlen Laboratory-Apoptosis, Cancer and Development, Centre de Recherche en Cancérologie, Lyon, France46 (link).
Lengrand J., Pastushenko I., Vanuytven S., Song Y., Venet D., Sarate R.M., Bellina M., Moers V., Boinet A., Sifrim A., Rama N., Ducarouge B., Van Herck J., Dubois C., Scozzaro S., Lemaire S., Gieskes S., Bonni S., Collin A., Braissand N., Allard J., Zindy E., Decaestecker C., Sotiriou C., Salmon I., Mehlen P., Voet T., Bernet A, & Blanpain C. (2023). Pharmacological targeting netrin-1 inhibits EMT in cancer. Nature, 620(7973), 402-408.
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10

Genetically Engineered Mouse Models

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K-RasLSL-G12D mice [28 (link)] and Shh-Cre mice [29 (link)] were purchased from the Jackson laboratory. Lmo4fl/fl mice were obtained from Prof Visvader (The Walter and Eliza Hall Institute, Australia). Scgb1a1-creER mice were a kind gift from Prof Hogan (Duke University) [30 (link)]. The Lmo4fl/fl and Lmo4LacZ KI/+ mice have been described by Hahm et al. [11 (link)]. All animal experiments were conducted according to the Melbourne Health Research Directorate Animal Ethics Committee guidelines. Mouse tail DNA were genotyped by PCR using the following primers: Lmo4-floxed: 5′-CGAGCTGCTGCCCGGATTCAC-3′, 5′-GCATTCACCAGCCACAGATAAG-3′ and 5′-CGAGCTGAAATTGTCAGCAGCAAG-3′; Shh-cre: 5′-TTCGGCTATACGTAACAGGG-3′, 5′-TCGATGCAACGAGTGATGAG-3′; K-RasLSL-G12D: 5′-CGCAGACTGTAGAGCAGCG-3′, 5′-CCATGGCTTGAGTAAGTCTGC-3′.
Holik A.Z., Filby C.E., Pasquet J., Viitaniemi K., Ciciulla J., Sutherland K.D, & Asselin-Labat M.L. (2015). The LIM-domain only protein 4 contributes to lung epithelial cell proliferation but is not essential for tumor progression. Respiratory Research, 16(1), 67.
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