Retinal protein extraction, SDS-PAGE and immunoblot analysis were performed as described previously70 (link). Briefly, a BCA protein assay was used to measure the quantity of protein. Protein from the retina was separated with 7.5% polyacrylamide gel (Bio Rad, USA) and transferred to a PVDF membrane (Merck-Millipore, Darmstudt, Germany). After blocking with 2% skim milk, the membrane was incubated in a blocking buffer containing rabbit anti-α-fodrin antibody (1:2000; Abcam) at room temperature for 1 h. The membrane was incubated with HRP-conjugated anti-rabbit IgG (dilution 1:5,000; Sigma). The immunoreactive band was developed with ECL prime (GE Healthcare, WI, USA) and examined with ChemiDoc XRS + (Bio-rad). As an internal control, the membrane was incubated with rabbit anti-β-actin antibody (dilution 1:1000; Sigma) at 4 °C overnight. The density of the immunoreactive band was then determined with a digital scanner and Image J software.
Rabbit anti α fodrin antibody
Manufactured by Abcam
Rabbit anti-α-fodrin antibody is a primary antibody that recognizes the α-fodrin protein. α-fodrin is a cytoskeletal protein involved in membrane-cytoskeleton interactions. This antibody can be used for the detection and analysis of α-fodrin in various experimental applications.
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2 protocols using rabbit anti α fodrin antibody
1
Retinal Protein Extraction and Analysis
2
Retinal Protein Analysis by SDS-PAGE and Immunoblot
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Retinal protein extraction, SDS-PAGE and an immunoblot analysis were performed as described previously.22 (link) Briefly, membranes were incubated in a blocking buffer containing rabbit anti-α-fodrin antibody (Abcam 1:2000) at room temperature for 1 h. The membranes were then incubated with HRP-conjugated anti-rabbit IgG (dilution 1:5000; Sigma), immunoreactive bands were developed with ECL prime (GE Healthcare, Life Sciences) and the bands were examined with ChemiDoc XRS+ (Bio-rad). As an internal control, membranes were incubated with rabbit anti-β-actin antibody (dilution 1:1000; Sigma) at 4° C overnight. The density of the immunoreactive bands was then determined with a digital scanner and Image J software.
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