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Ms 550d 40 mhz

Manufactured by Fujifilm
Sourced in Canada

The MS-550D 40 MHz is a lab equipment product from Fujifilm. It operates at a frequency of 40 MHz.

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4 protocols using ms 550d 40 mhz

1

Ultrasound-Guided Liposome Delivery to Sciatic Nerve

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The Visualsonics Vevo 2100 (VisualSonics Inc., Toronto, ON, Canada) with an MS-550D 40 MHz or an MS-250 20 MHz transducer at B-mode was used for sonography. In vitro imaging was achieved by applying 100 µL of liposome or water on top of a layer of ultrasonic gel, and covering it with another layer of gel. The top layer gel was in contact with the transducer.
Sprague–Dawley rats of 300 – 400 g were anesthetized with isoflurane-oxygen and positioned under the ultrasound transducer. Ultrasonic gel was applied between the transducer and the rat. A 23G needle was used to inject 200 µL of Lipo-PPIX. The needle was placed beside the sciatic nerve as shown from the real-time sonogram prior to liposome administration (Fig. S6).
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2

Ultrasound-Guided Liposome Delivery to Sciatic Nerve

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The Visualsonics Vevo 2100 (VisualSonics Inc., Toronto, ON, Canada) with an MS-550D 40 MHz or an MS-250 20 MHz transducer at B-mode was used for sonography. In vitro imaging was achieved by applying 100 µL of liposome or water on top of a layer of ultrasonic gel, and covering it with another layer of gel. The top layer gel was in contact with the transducer.
Sprague–Dawley rats of 300 – 400 g were anesthetized with isoflurane-oxygen and positioned under the ultrasound transducer. Ultrasonic gel was applied between the transducer and the rat. A 23G needle was used to inject 200 µL of Lipo-PPIX. The needle was placed beside the sciatic nerve as shown from the real-time sonogram prior to liposome administration (Fig. S6).
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3

Echocardiographic Imaging of Mouse Cardiac Function

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Echocardiography was performed on a VisualSonics’ Vevo 2100 ultrasound machine using an MS550D (40 MHz) transducer22 (link). Briefly, mice were anesthetized in an induction chamber filled with 3.5% isoflurane mixed with 100% oxygen and then placed supine on a heated stage containing ECG leads. The heated stage also contained a nosecone filled with 1.5% isoflurane in 100% oxygen delivered at 1.0 L/min and an anal temperature probe by which temperature was maintained at 37° C. Transthoracic B-mode, M-mode, pulsed Doppler and tissue Doppler images were obtained from the parasternal short axis view (systolic and morphology parameters) and the apical view (diastolic parameters). Results are based on the average of at least three cardiac cycles.
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4

Glycerol for Neonatal Cranial Hemorrhage

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In experiment A, the animals were injected s.c. after birth with a single bolus (100 μl) of sterile isotonic saline (0.9% NaCl, B Braun, Melsungen, Germany) to mimic the injections in experiment B. At approximately 5 h after birth, animals were monitored with HFU (Vevo 2100, VisualSonics Inc., Toronto, ON, Canada) with an MS-550D 40 MHz transducer to scan for any spontaneous cranial bleedings as described previously21 (link). All animals without signs of cerebral hemorrhage received a single bolus of 50% (v/v) sterile glycerol solution (6.5 g/kg, Teknova, Hollister, US) given as an i.p. injection at 6, 12, 18, or 24 h after birth, in accordance with the allocation, after randomizing pups from nine does as described above, to respective experimental group. Two consecutive ultrasound scans were performed at 12 and 24 h post-glycerol administration. Animals were terminated 24 h post-glycerol administration, corresponding to a postnatal age of 30 (6 h glycerol administration group), 36 (12 h glycerol administration group), 42 (18 h glycerol administration group) or 48 (24 h glycerol administration group) hours, respectively.
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