Mjc13

MJC13 was custom synthesized (purity ≥ 99%) by Chembridge (San Diego, CA). Itraconazole (purity ≥ 98%), formic acid, HPLC grade acetonitrile and water were purchased from Sigma-Aldrich (St. Louis, MO). All chemicals and reagents were used as received. Freshly obtained blank rat plasma and urine were collected from male Sprague–Dawley rats (Harlan Laboratories, Houston, TX) and frozen at −80 °C till use.

MJC13 was custom synthesized (purity ≥ 99%) by Chembridge (San Diego, CA). Water, ethanol, propylene glycol, polyethylene glycol 400 (PEG 400), polysorbate 80 (Tween 80), olive oil, peanut oil, soybean oil, dimethyl sulfoxide (DMSO), 1-octanol, 0.9% sodium chloride solution (normal saline), fetal bovine serum (FBS), and 0.25% (w/v) Trypsin-0.53mM EDTA solution were purchased from Sigma-Aldrich (St. Louis, MO). Captex^® 200 was from ABITEC (Columbus, OH). Labrafac™ Lipophile WL1349 was from Gattefosse (Lyon, France). All chemicals and reagents were used as received. The 22Rv1 human prostate carcinoma epithelial cell line and its base medium RPMI-1640 were purchased from ATCC (Manassas, VA). C.B-17 SCID mice (male, 15–20 g) were purchased from Charles River Laboratories (Wilmington, MA).