Extractions were performed using a sonicator (VWR Ultrasound cleaner, model-USC 1700D). Analytical TLC was carried out with silica gel 60 F254 precoated aluminum sheets (Merck Art. 1.05554). Compounds on TLC were located using a UV lamp and by heating after spraying with acidic anisaldehyde. Silica gel (Fluka 60741, Merck Art. 7734 and 9385) and Sephadex LH-20 were used for column chromatography. Preparative thin layer chromatography (PTLC) was carried out using silica gel 60 F254 precoated glass plates (Merck Art 1.05715). 1H NMR and 13C NMR were recorded on a Bruker DRX500 (500 MHz for 1H and 125 MHz for 13C) or JEOL JMN-AL300 (300 MHz for 1H and 75 MHz for 13C) spectrometer in CD3OD or CDCl3 solution at 20 °C. Optical rotations were measured on a JASCO P-2200 polarimeter. IR spectra were measured on a JASCO FT/IR-460 spectrometer. UV spectra were recorded on a JASCO MD-4017 photo diode array detector. Positive-ion FABMS were obtained on a JEOL JMX-AX505HA spectrometer.
Silica gel 60 f254 precoated glass plates
Manufactured by Merck Group
Silica gel 60 F254 precoated glass plates are an analytical tool used in thin-layer chromatography (TLC) for the separation and identification of chemical compounds. The plates are coated with a thin layer of silica gel, which acts as the stationary phase. The F254 indicator allows for the visualization of separated compounds under ultraviolet light.
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Lab products found in correlation
3 protocols using silica gel 60 f254 precoated glass plates
1
Detailed Isolation and Characterization Protocol
2
Thin Layer Chromatography and NMR Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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General Protocols:
Thin layer chromatography was carried out on Merck silica gel 60 F254 precoated glass plates.
Compounds were visualized under a UV lamp. All NMR spectra were recorded on Bruker Avance III HD Ascend 600 MHz using deuterated DMSO as solvent.
Thin layer chromatography was carried out on Merck silica gel 60 F254 precoated glass plates.
Compounds were visualized under a UV lamp. All NMR spectra were recorded on Bruker Avance III HD Ascend 600 MHz using deuterated DMSO as solvent.
3
Thin Layer Chromatography and NMR Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
General Protocols:
Thin layer chromatography was carried out on Merck silica gel 60 F254 precoated glass plates.
Compounds were visualized under a UV lamp. All NMR spectra were recorded on Bruker Avance III HD Ascend 600 MHz using deuterated DMSO as solvent.
Thin layer chromatography was carried out on Merck silica gel 60 F254 precoated glass plates.
Compounds were visualized under a UV lamp. All NMR spectra were recorded on Bruker Avance III HD Ascend 600 MHz using deuterated DMSO as solvent.
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