Log In Sign up
The largest database of trusted experimental protocols

Hy 11109

Manufactured by MedChemExpress
Visit Supplier
Sourced in United States

HY-11109 is a laboratory equipment product offered by MedChemExpress. It is a device designed for general laboratory use. The core function of HY-11109 is to facilitate various experimental procedures and analyses conducted in a laboratory setting.

Automatically generated - may contain errors

Lab products found in correlation

Ribofect cp transfection kit, by RiboBio (2 mentions) Hy n1966, by MedChemExpress (2 mentions) Hy 120697, by MedChemExpress (2 mentions) Dm2500, by Leica (1 mentions) Hy b0215, by MedChemExpress (1 mentions) Hy 100461, by MedChemExpress (1 mentions) Tak 242, by MedChemExpress (1 mentions) A3803, by Merck Group (1 mentions)

Spelling variants of this product

TAK-242 (HY-11109) (4 citations)

6 protocols using hy 11109

1

Neutrophil NETosis Inhibition Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunofluorescence staining, freshly isolated polymorphonuclear neutrophils (PMNs) from the bone marrow were seeded on poly-d-lysine-coated cover slips and allowed to adhere. Cells were treated with NAC (100 μm) (HY-B0215, MedChemExpress) and TAK242 (10 μm) (HY-11109, MedChemExpress) for 3 h and then stimulated with LPS (10 ng/mL) + L-OHP (10 μΜ) for 4 h. Cells were further fixed for 12 h with 4% paraformaldehyde (PFA) and blocked with 1% BSA and 0.3% Triton X-100 in PBS for 30 min. Then, rabbit anti-H3Cit and mouse anti-MPO primary antibodies were used overnight at 4 °C. After three washes, Alexa Fluor 488 donkey anti-rabbit IgG and Alexa Fluor Cy3 donkey anti-mouse IgG were added for 1.5 h at room temperature. Neutrophil-derived NET formation was visualized by fluorescence microscopy (LEICA DM2500).
Wang C.Y., Lin T.T., Hu L., Xu C.J., Hu F., Wan L., Yang X., Wu X.F., Zhang X.T., Li Y., Yin H.Y., Jiang C.Y., Xin H.L, & Liu W.T. (2023). Neutrophil extracellular traps as a unique target in the treatment of chemotherapy-induced peripheral neuropathy. eBioMedicine, 90, 104499.
+ Open protocol
+ Expand
2

Keratinocyte Scratch Assay with OA-RD17, miR-632, and Inhibitors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell scratch assays were performed to investigate the effects of OA-RD17 (1 nM), miR-632 mimic (50 nM), and miR-632 inhibitor (100 nM) (riboFECT™ CP Transfection Kit, RiboBio, China) on keratinocyte scratch repair following previous study [16 (link)]. In addition, the underlying mechanisms of OA-RD17- and miR-632-induced keratinocyte scratch healing were determined using a specific TLR4 inhibitor (1 μM, HY-11109, MedChemExpress, USA), MAPK signaling pathway inhibitor (1 μM, HY-N1966, MedChemExpress, USA), or β-catenin inhibitor (10 μM, HY-120697, MedChemExpress, USA).
Li C., Fu Z., Jin T., Liu Y., Liu N., Yin S., Wang Z., Huang Y., Wang Y., Zhang Y., Li J., Wu Y., He L., Tang J., Wang Y, & Yang X. (2023). A frog peptide provides new strategies for the intervention against skin wound healing. Cellular & Molecular Biology Letters, 28, 61.
+ Open protocol
+ Expand
3

Toll-like Receptor Inhibition in BMDM Cytokine Response

Check if the same lab product or an alternative is used in the 5 most similar protocols
BMDM cells were harvested from the femurs and tibia of C57BL6/J mice. The culture medium used to differentiate and maintain BMDM cells was DMEM supplemented with 10% (v/v) FBS, 1% penicillin/streptomycin, and 10% L929 cell supernatant. BMDMs were seeded in 6-well tissue culture plates at day 7. To identify which TLR was critical in NET-triggered cytokine production, we pretreated BMDMs with inhibitors of TLR2 (C29, HY-100461, MedChemExpress), TLR4 (TAK242, HY-11109, MedChemExpress), TLR7 (IRS661, Genepharma), TLR8 (CU-CPT9a, HY-11266, MedChemExpress), or TLR9 (IRS869, Genepharma) for 1 hour and then stimulated them with NETs (500 ng/mL) for 3 hours. The levels of Il1b, Il6, and Tnfa mRNA were measured by quantitative real-time PCR analysis (n = 3; see “Quantitative real-time PCR analysis”). IRS661 sequence: 5′-TGCTTGCAAGCTTGCAAGCA-3′. IRS869 sequence: 5′-TCCTGGAGGGGTTGT-3′.
Lin T., Hu L., Hu F., Li K., Wang C.Y., Zong L.J., Zhao Y.Q., Zhang X., Li Y., Yang Y., Wang Y., Jiang C.Y., Wu X, & Liu W.T. (2022). NET-Triggered NLRP3 Activation and IL18 Release Drive Oxaliplatin-Induced Peripheral Neuropathy. Cancer Immunology Research, 10(12), 1542-1558.
+ Open protocol
+ Expand
4

Extracellular Vesicle Protocols for Keratinocyte Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols
For EVP treatment, KCs were treated with PBS or 10 μg/ml of EVPs derived from cell lines or tissue explants for 4 h; or pretreated with DMSO or 5 μM of TAK (MedChemExpress, HY-11109) for 1 h, followed by treatment with EVPs (10 μg/ml) in the presence or absence of TAK (5 μM) for 4 h. KCs were then collected for RNA extraction and qRT-PCR analysis.
For TPA treatment, KCs were treated with DMSO or 0.2 μM of TPA for 4h, followed by RNA extraction and qRT-PCR analysis.
For palmitic acid (PA) treatment, stock PA (Millipore Sigma, P5585) was made up in 100% ethanol at 200 mM and diluted into 0.2 μm-filter sterilized PA-carrier medium (DMEM containing 5% EVP-depleted FBS, 5% fatty acid-free BSA [Sigma Aldrich, A3803], 1× non-essential amino acids solution, 1× penicillin/streptomycin and 1× L-Glutamine) at a final concentration of 200 μM. As a control, 100% ethanol was diluted into PA-carrier medium at 1:1000 dilution. To facilitate the conjugation of PA to BSA, the mixture was incubated at 37 °C for 30 min with gentle shaking. KCs were pretreated with DMSO or 5 μM of TAK for 1 h, washed once with PBS and then incubated in medium carrying 200 μM of PA or control ethanol with or without TAK (5 μM) for 4 h. KCs were then collected for RNA extraction and qRT-PCR analysis.
Wang G., Li J., Bojmar L., Chen H., Li Z., Tobias G.C., Hu M., Homan E.A., Lucotti S., Zhao F., Posada V., Oxley P.R., Cioffi M., Kim H.S., Wang H., Lauritzen P., Boudreau N., Shi Z., Burd C.E., Zippin J.H., Lo J.C., Pitt G.S., Hernandez J., Zambirinis C.P., Hollingsworth M.A., Grandgenett P.M., Jain M., Batra S.K., DiMaio D.J., Grem J.L., Klute K.A., Trippett T.M., Egeblad M., Paul D., Bromberg J., Kelsen D., Rajasekhar V.K., Healey J.H., Matei I.R., Jarnagin W.R., Schwartz R.E., Zhang H, & Lyden D. (2023). Tumor extracellular vesicles and particles dysregulate liver metabolism. Nature, 618(7964), 374-382.
+ Open protocol
+ Expand
5

Aging and Liver Fibrosis: Interventional Strategies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Male C57BL/6 mice aged 8–12 weeks (young group) and aged 18–20 months (aged group) were purchased from Hangzhou Ziyuan Laboratory Animal Technology Co., Ltd. (Zhejiang, China). The mice were housed at 22–24°C with a 12-hour daylight/darkness cycle. The Animal Care Committee of Anhui Medical University (AHMU) approved all experimental procedures, and animal welfare was performed under relevant regulations (approval number LLSC2022130). To induce liver fibrosis in mice, 10% CCl4 (289116, Sigma)-olive oil solution (5 mL/kg, thrice a week) was given intraperitoneally for 4 weeks. YC-1 (10 mg/kg, every other day, S7958, Selleck), glycyrrhizin (50 mg/kg, daily, HY-N0184, MedChemExpress), TAK-242 (10 mg/kg, daily, HY-11109, MedChemExpress), or vehicle saline were given intraperitoneally for 2 weeks after 2 weeks of CCl4 intoxication.
Dai Q., Qing X., Jiang W., Wang S., Liu S., Liu X., Huang F, & Zhao H. (2023). Aging aggravates liver fibrosis through downregulated hepatocyte SIRT1-induced liver sinusoidal endothelial cell dysfunction. Hepatology Communications, 8(1), e0350.
+ Open protocol
+ Expand
6

Proliferative Effects of OA-RD17 and miR-632 on Skin Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The pro-proliferative effects of OA-RD17 (1 nM) on keratinocytes, macrophages, primary macrophages, and primary keratinocytes were determined according to previous study [16 (link)]. The effects of miR-632 mimic (50 nM), and miR-632 inhibitor (100 nM) (riboFECT™ CP Transfection Kit, RiboBio, China) on keratinocyte proliferation were explored according to the manufacturer’s instructions. The underlying mechanism of OA-RD17-induced proliferation against keratinocyte and macrophage was investigated using a specific MAPK signaling pathway inhibitor (10 μM, HY-N1966, MedChemExpress, USA). Furthermore, the mechanisms related to OA-RD17- and miR-632-induced proliferation of keratinocyte were detected using a specific TLR4 inhibitor (1 μM, HY-11109, MedChemExpress, USA) or β-catenin inhibitor (10 μM, HY-120697, MedChemExpress, USA).
Li C., Fu Z., Jin T., Liu Y., Liu N., Yin S., Wang Z., Huang Y., Wang Y., Zhang Y., Li J., Wu Y., He L., Tang J., Wang Y, & Yang X. (2023). A frog peptide provides new strategies for the intervention against skin wound healing. Cellular & Molecular Biology Letters, 28, 61.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!