Dimethylsulfoxide (DMSO), IPTG (isopropylthio-β-galactoside), ampicillin and WST-1 reagent were obtained from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals were purchased from Applichem (Darmstadt, Germany) unless otherwise specified. All solvents and components of buffer solutions were of analytical grade and used as received. Monoclonal antibodies to TRAIL (MAB375) were from R&D systems (Minneapolis, MN, USA), and anti-DR5 monoclonal antibodies (DR5-01-1) and secondary antibodies Dylight 488 were from GeneTex (Irvine, CA, USA). Bacteria were cultivated using Gibco Bacto yeast extract and Gibco Bacto tryptone (Thermo Fisher Scientific, Waltham, MA, USA). Human colorectal carcinoma HCT116 and HT29 and human glioblastoma U-87 and T98G cell lines were from ATCC (Washington, DC, USA). Cell culture media (DMEM, RPMI1640), 0.05% trypsin-EDTA solution and phosphate-buffered saline tablets were from PanEco (Moscow, Russia). Fetal bovine serum was from HyClone (Cramlington, UK).
Gibco bacto tryptone
Manufactured by Thermo Fisher Scientific
Sourced in United States
Gibco Bacto tryptone is a high-quality peptone derived from the enzymatic digestion of casein. It is a widely used component in the preparation of microbiological culture media, providing essential nutrients for the growth and maintenance of various bacteria, yeasts, and other microorganisms.
Automatically generated - may contain errors
Lab products found in correlation
Ampicillin, by Merck Group (2 mentions)
Wst 1 reagent, by Merck Group (2 mentions)
Dylight 488, by GeneTex (2 mentions)
Gibco bacto yeast extract, by Thermo Fisher Scientific (2 mentions)
Phosphate buffered saline tablets, by PanEco (2 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Ht 29, by American Type Culture Collection (1 mentions)
Omnitray, by Thermo Fisher Scientific (1 mentions)
Alamarblue reagent, by Thermo Fisher Scientific (1 mentions)
Pan caspase inhibitor z vad fmk, by Santa Cruz Biotechnology (1 mentions)
Cc mount tissue mounting medium, by Merck Group (1 mentions)
Mouse igg1 15h6, by GeneTex (1 mentions)
Sulfo cyanine3 maleimide, by Lumiprobe (1 mentions)
4 protocols using gibco bacto tryptone
1
TRAIL and DR5 Immunodetection Protocol
2
Bacterial Motility Assay on Agar
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The indicated bacterial strains were streaked onto fresh LBS plates with the appropriate antibiotics and grown overnight at 25°C. Single colonies were picked with a sterile toothpick and deposited onto OmniTrays (Thermo Fisher Scientific; catalog no. 242811) containing TBS agar (per liter, 10 g Gibco Bacto tryptone [Thermo Fisher Scientific; catalog no. 211705], 50 ml of 1 M Tris buffer [pH 7.]0, 20 g NaCl, 8.63 g MgSO4, and 3 g agar, in distilled water) by stabbing the toothpick into the media at a single spot. The trays were incubated at 28°C for 4 h, and the outer diameter of swimming cells was measured.
3
Genetic Modification of E. coli Strains
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The genetically modified strains of E. coli B used in this study were the strains IC188 (WP2uvrA oxyR+/pKM101), IC203 (WP2uvrA ΔoxyR30/pKM101) and IC5233 (WP2uvrA ΔoxyR30 sodAB-/pKM101). All of these strains were obtained from the collection of the former Instituto de Investigaciones Citológicas of Valencia, now hosted in the Príncipe Felipe Research Center (Valencia, Spain) [18 (link),19 (link)]. Overnight cultures were prepared from 100 μL of frozen permanent cultures inoculated into 9 mL of LB medium: NaCl, 10 g/L; Gibco Bacto tryptone (Thermo Fisher, Waltham, MA, USA), 10 g/L; Gibco Yeast extract (Thermo Fisher, Waltham, MA, USA), 50 g/L and incubated for 24 h at 37 °C. PROVIDERS OF MEDIA
4
Optimizing Glioblastoma Cell Assays
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The ampicillin, IPTG (isopropyl-β-d-1-thiogalactopyranoside), WST-1 reagent, and CC/Mount tissue-mounting medium were obtained from Sigma-Aldrich (St. Louis, MO, USA); the Alamar Blue reagent was from Thermo Fisher Scientific (Waltham, MD, USA); the iRGD peptide was from InvivoChem (Libertyville, IL, USA); sulfo-Cyanine 3 maleimide was from Lumiprobe (Moscow, Russia); pan-caspase inhibitor Z-VAD-FMK was from Santa Cruz Biotechnology (Dallas, TX, United States). All other chemicals were obtained from Applichem (Darmstadt, Germany) unless otherwise specified. All solvents and components of buffer solutions were of analytical grade. Monoclonal antibodies to TRAIL (MAB375) were from R&D systems (Minneapolis, MN, USA); monoclonal antibodies to DR5 (DR5-01-1) and integrin αVβ3 (23C6), secondary antibodies Dylight 488 and mouse IgG1 (15H6) were from GeneTex (Irvine, CA, USA). E. coli SHuffle B T7 cells were from New England Biolabs (Ipswich, MA, USA. Bacterial cells were cultivated using Gibco Bacto yeast extract and Gibco Bacto tryptone (Thermo Fisher Scientific, Waltham, MA, USA). Human glioblastoma U87 and T98G cells were from ATCC (Washington, DC, USA). The cell culture media DMEM, 0.25% Trypsin-Versene solution, and phosphate-buffered saline tablets were from PanEco (Moscow, Russia). Thefetal bovine serum was from HyClone (Cramlington, UK).
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