H3k27me2 3

Manufactured by Active Motif

H3K27me2/3 is a laboratory product that specifically detects dimethylated and trimethylated histone H3 at lysine 27 (H3K27me2/3). It is used to study epigenetic modifications and chromatin regulation.

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Lab products found in correlation

Steponeplus real time pcr system, by Thermo Fisher Scientific (1 mentions) Hiseq 4000, by Illumina (1 mentions) Nextseq 500, by Illumina (1 mentions) H3k27me3, by Merck Group (1 mentions) Perfecta sybr green fastmix, by Thermo Fisher Scientific (1 mentions) H3k36me2, by Abcam (1 mentions) H3k27ac, by Active Motif (1 mentions) H3k36me3, by Abcam (1 mentions) Subcellular protein fractionation kit, by Thermo Fisher Scientific (1 mentions) Criterion gel, by Bio-Rad (1 mentions) Odyssey imaging system, by LI COR (1 mentions) Tfiib, by Cell Signaling Technology (1 mentions) Vdac1, by GenScript (1 mentions)

Spelling variants of this product

H3K27me2 (3 citations) H3K27me2/3 antibody (2 citations)

2 protocols using h3k27me2 3

ChIP-seq and qPCR Analysis of Chromatin Modifications

Cited 4 times

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ChIP was performed as previously described (Jamieson et al., 2016 (link)). qPCR was performed using the Quanta Biosciences PerfeCTa Sybr Green FastMix and an Applied Biosystems Step One Plus Real-Time PCR System. ChIP-libraries were prepared as previously described (Jamieson et al., 2016 (link)) and sequencing was performed using an Illumina NextSeq 500 or HiSeq 4000 sequencer with 75- or 100-nt single-end reads, respectively. All sequencing reads were mapped to the corrected N. crassa OR74A (NC12 genome) (Galazka et al., 2016 (link)) using Bowtie2 (Langmead and Salzberg, 2012 (link)). ChIP-seq read coverage was averaged, normalized, and analyzed using tools available from deepTools2 (Ramírez et al., 2016 (link)) and SAMtools (Li et al., 2009 (link)) on the open-source platform Galaxy (Afgan et al., 2016 (link)). Sequencing tracks are displayed as 25-nt-window TDF or bigWig files with the Integrative Genomics Viewer (IGV) (Robinson et al., 2011 (link)). The following antibodies were used for ChIP: H3K27me3 (Millipore, Cat#07 – 449), H3K36me3 (Abcam, Cat#ab9050), H3K36me2 (Abcam, Cat#ab9049), H3K27ac (ActiveMotif, Cat#39133), H3K27me2/3 (ActiveMotif, Cat#39535).

Bicocca V.T., Ormsby T., Adhvaryu K.K., Honda S, & Selker E.U. (2018). ASH1-catalyzed H3K36 methylation drives gene repression and marks H3K27me2/3-competent chromatin. eLife, 7, e41497.

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Subcellular Protein Fractionation and Western Blotting

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Proteins were fractionated using the subcellular protein fractionation kit (Thermo Fisher, cat # 78840), according to the manufacturer’s protocols. The cellular fractions were separated on a 4% to 12% gradient SDS-PAGE (Criterion gels, Bio-Rad) and transferred to nitrocellulose membrane. The antibodies used include: anti-turboGFP (Origene, cat # TA150041), -OGDH (E1W8H, Cell Signaling Technology, cat # 26865), -ACAA2 (Origene Technologies, cat # TA506126), -H2A.Z (Active Motif, cat # 39943), -H3 (Active Motif, cat # 61476), -H3 pan-acetyl (Active Motif, cat # 39140), -H3K27 di- and tri-methyl (H3K27me2/3, Active Motif, cat # 39538), -nuclear pore glycoprotein p62 (NUP62, US Biological, cat # USB326547), -transcription factor II B (TFIIB, Cell Signaling Technology, cat # 4169), -H4K12-succinyl (H4K12suc, Epigentek, cat # A70383), -H2A (Active Motif, cat # 35951), -H4 (Upstate, cat # 07-108), -H3K9 mono-, di-, tri-methyl (H3K9me1/2/3, Active Motive, cat # 38241), -AKT1 (Cell Signaling Technology, cat # 9272), -voltage-dependent anion-selective channel 1 (VDAC1, Genscript, cat # A01419), -RNA pol II (Abcam, cat # ab5095), and -complex IV (Thermo Scientific, Cat# 459600). The Western blot signals were detected by the Odyssey imaging system (LI-COR).

Choi S., Pfleger J., Jeon Y.H., Yang Z., He M., Shin H., Sayed D., Astrof S, & Abdellatif M. (2019). Oxoglutarate dehydrogenase and acetyl-CoA acyltransferase 2 selectively associate with H2A.Z-occupied promoters and are required for histone modifications. Biochimica et biophysica acta. Gene regulatory mechanisms, 1862(10), 194436.

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