Chromatographic acetonitrile

The 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2, 4, 6-Tris(2-pyridyl)-s-triazine (TPTZ), 2, 6-di-tert-butyl-4-methylphenol (BHT), vitamin C (Vc), were obtained from Solarbio Life sciences (Beijing, China). All chemical reagents including methanol, ethyl acetate (EtOAc), petroleum ether (PE), Dichloromethane (DCM), and n-butanol (n-BuOH) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Chromatographic acetonitrile was purchased from Merck (Darmstadt, Germany). Folin-Ciocalteu, pyrogallol, and other analytical grade chemicals were purchased from Aladdin (Shanghai, China). Myricetin, dihydroMyricetin, quercetin, resveratrol, apigenin, baicalein, hesperitin, gallic acid, rutin, luteolin, epicatechin, epicatechin gallate, and other standards for HPLC were purchased from Yuanye Bio-Technology (Shanghai, China). Cellulase (3, 000 μ/g) and pectinase (40, 000 μ/g) were purchased from Xiya reagent (Shandong, China). α-Glucosidase (G5003) and acetylcholinesterase (AChE, C3389) were purchased from Sigma-Aldrich (St. Louis, USA). p-Nitrophenol nitrophenol α-D-glycopyranoside (pNPG), acarbose, galantamine, and cisplatin were purchased from Sigma-Aldrich (St. Louis, MO, USA). The HepG2, MCF-7, Raw264.7, and A549 cells were purchased from Conservation genetics CAS Kunming cell bank.

The 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH), fluorescein sodium, acetylthiocholine iodide, vitamin C (Vc), and 2,6-di-tert-butyl-4-methylphenol (BHT) were obtained from Aladdin Biotechnology (analytical grade, China). Other analytical grade chemicals, including methanol, petroleum ether, ethyl acetate, and n-butanol were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
Chromatographic acetonitrile was purchased from Merck (Darmstadt, Germany). α-glucosidase (from saccharomyces cerevisiae), xanthine oxidase (XO), acetylcholinesterase (AChE), acarbose, galantamine (GALM), allopurinol, 4-methylumbelliferyl-β-D glucuronide (4-MUG), 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB), and acetylthiocholine iodide (ATCI) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

All chemical reagents including methanol, petroleum ether (PE), chloroform (CF), ethyl acetate (EtOAc), and n-butanol (n-BuOH) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Folin-Ciocalteu, pyrogallol, and other analytical grade chemicals were purchased from Aladdin (Shanghai, China). Chromatographic acetonitrile was purchased from Merck (Darmstadt, Germany). Gallic acid and other standards for HPLC were purchased from Yuanye Bio-Technology (Shanghai, China). Cellulase (3000 U/g) and pectinase (40,000 U/g) were purchased from Xiya reagent (Shandong, China). α-glucosidase from Saccharomyces cerevisiae (G5003), acarbose, 4-methylumbelliferyl α-D-glucopyranoside (4-MUG), acetylcholinesterase (AChE) from Electrophorus electricus (C3389), galanthamine (GAL), acetylthiocholine iodide (ATCI), 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB), tyrosinase from mushroom (T3824), kojic acid, 3,4-Dihydroxy-L-phenylalanine (L-dopa), cisplatin, and streptozocin (STZ) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
The MCF-7 and HepG2 cells were purchased from Conservation genetics CAS Kunming cell bank. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and other cell culture reagents were purchased from Gibco (Carlsbad, CA, USA). Cell counting kit-8 (CCK-8) reagent was purchased from Dojindo (Kumamoto, Japan).