All cell lines, HT-29, SK-CO-1, NCI-H747, and HCC-827, were purchased from ATCC (Manassas, VA, USA). ATCC authenticates all its cell lines by short tandem repeat profiling, cell morphology monitoring, karyotyping, and cytochrome C oxidase I testing. The cells were grown in ATCC-recommended media, containing 10% FBS and 1% Penicillin-streptomycin (Thermo Fisher Scientific, Pittsburgh, PA, USA). All cells were cultured at 37 °C in humid atmosphere containing 5% CO2. Kinase inhibitors were purchased from Selleckchem (Munich, Germany), LC Laboratories (Woburn, MA, USA) or AdooQ Bioscience (Irvine, CA, USA).
Nci h747
Manufactured by American Type Culture Collection
Sourced in United States
The NCI-H747 is a cell line derived from a human colon adenocarcinoma. It is maintained in cell culture and can be used for various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Sk co 1, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Ht 29, by American Type Culture Collection (1 mentions)
Hcc827, by American Type Culture Collection (1 mentions)
Kinase inhibitors, by Selleck Chemicals (1 mentions)
Hca 7, by American Type Culture Collection (1 mentions)
Skov3, by American Type Culture Collection (1 mentions)
Bt474, by American Type Culture Collection (1 mentions)
Cal27, by American Type Culture Collection (1 mentions)
Hcc1954, by American Type Culture Collection (1 mentions)
Au565, by American Type Culture Collection (1 mentions)
Hcc2218, by American Type Culture Collection (1 mentions)
3 protocols using nci h747
1
Cell Line Authentication and Culture
2
Cell line cultivation protocol
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HeLa, NCI-H747, and HCA7 were obtained from ATCC (USA) and CW-2 cells from RIKEN bioresource center (Japan). DMEM was used for HeLa and HCA7. RPMI-1640 was used for NCI-H747 and CW-2. All media contained 10% FBS, 2 mM glutamine, 50 I.U./ml penicillin, and 50 μg/ml streptomycin. The cell lines were tested for mycoplasma contamination.
3
Cell Line Procurement and Maintenance
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BT474, HCC1954, SKOV3, HCC2218, AU565, Toledo, Raji, Jurkat, FaDu, NCI.H747, SW48, and Cal27 were purchased from ATCC in either 2020 or 2021. ATCC has confirmed the identity of human cell lines by (short tandem repeat analyses. Cells were cultured and maintained in media supplemented with 10% or 20% of FBS per ATCC's recommendation. Fresh stocks of cells were made on early passages and fresh culture of each line was started every 2–3 months for in vitro assays. Mycoplasma testing was performed on cancer cell lines on July 6, 2022. Primary macrophages were not assessed for Mycoplasma.
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