Acrylamide

Manufactured by Maclin Biochemical Technology

Sourced in China

Acrylamide is a chemical compound commonly used in various laboratory applications. It serves as a basic building block for the synthesis of polyacrylamide gels, which are widely employed in gel electrophoresis techniques. The primary function of acrylamide is to facilitate the separation and analysis of biomolecules, such as proteins and nucleic acids, based on their size and charge characteristics.

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Lab products found in correlation

Close spelling variants of this product

N′-methylenebis(acrylamide), (3 citations) 2-acrylamide-2-methylpropanesulfonic acid (2 citations) Acrylamide (AM, (2 citations) N,N-Methylenebisacrylamide Bis-acrylamide (2 citations)

19 protocols using acrylamide

Synthesis of Nitrogenous Compounds

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5-Cyanovaleramide was bought
from Toronto Research Chemicals Inc., Canada. Malonamide and cyanoacetamide
were purchased from Shanghai Aladdin Bio-Chem Technology Co., Ltd.
Adiponitrile, adipamide, malononitrile, terephthalonitrile, 4-cyanobenzamide,
terephthalamide, 3-cyanopyridine, nicotinamide, acrylonitrile, acrylamide,
benzonitrile, and benzamide were obtained from Shanghai Macklin Biochemical
Co., Ltd. All the reagents are analytical grade or higher.

Wang L., Liu S., Du W., Dou T, & Liang C. (2020). High Regioselectivity Production of 5-Cyanovaleramide from Adiponitrile by a Novel Nitrile Hydratase Derived from Rhodococcus erythropolis CCM2595. ACS Omega, 5(29), 18397-18402.

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Polymer-based Photocrosslinkable Hydrogel Synthesis

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Acrylamide (Am, 99.9%), acryloyl chloride (96%), choline chloride (ChCl, 99%), β-cyclodextrin (β-CD, 98%), dichloromethane (DCM, 99.9%, with molecular sieves, water ≤ 50 ppm), 4-dimethylaminopyridine (DMAP, 99%), N,N-dimethylformamide (DMF, 99.8%, with molecular sieves, water ≤ 50 ppm), 4-(4,6-dimethoxy−1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM, 97%), ethylene glycol (EG, 98%), poly(ethylene glycol) (PEG, average Mn = 2000), rhodamine B, and tartrazine (yellow dye) were purchased from Macklin. 2-Hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (I₂₉₅₉, ≥98.0%), lithium phenyl(2,4,6-trimethylbenzoyl)phosphinate (LAP, ≥98.0%), lithocholic acid (LCA, >97.0%), N,N’-methylenebisAcrylamide (MBA), sodium bicarbonate (NaHCO₃, ≥99.8%), and triethylamine (TEA, ≥99.5%) were purchased from Aladdin. Acetone, diethyl ether and hydrochloric acid (HCl) were purchased from Guangzhou Chemical Reagent Factory (Guangdong, China).

Xiong X., Chen Y., Wang Z., Liu H., Le M., Lin C., Wu G., Wang L., Shi X., Jia Y.G, & Zhao Y. (2023). Polymerizable rotaxane hydrogels for three-dimensional printing fabrication of wearable sensors. Nature Communications, 14, 1331.

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Anthraquinone-Based Metal Complexes Synthesis

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1,4-dihydroxyanthraquinone, CoCl₂, NaCl, CuSO₄, ZnCl₂, MgCl₂, HgCl₂, Ni(NO₃)₂, MnCl₂, NH₄Cl, Al₂(SO₄)₃, CoCl₂, formamide, ethanol, dichloromethane (DCM), glyphosate (GP), trichlorfon, 1-naphthofen, carbaryl, carbendazim, polyvinyl alcohol (PVA), acrylamide, ammonium persulphateetc, and all of the reagents were purchased from Shanghai Macklin Biochemical Co., Ltd (http://www.macklin.cn/) and were used without further treatment.

Wu J., Chen X., Zhang Z, & Zhang J. (2022). “Off-on” fluorescence probe based on green emissive carbon dots for the determination of Cu2+ ions and glyphosate and development of a smart sensing film for vegetable packaging. Mikrochimica Acta, 189(3), 131.

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Polymer Microsphere Fabrication Protocol

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Acrylamide (AM) was supplied by Macklin (Shanghai, China). Potassium persulfate, trichloromethane and isopropyl alcohol were purchased from Sinopharm Chemical Reagent (Shanghai, China). Potassium sucrose octasulfate was purchased from Avito (Shanghai, China). Polyvinyl alcohol (PVA), methyl methacrylate (MMA), Span-80, divinylbenzene (DVB) and all other chemicals were purchased from Aladdin (Shanghai, China). Cell counting kit 8 was purchased from Biosharp (Wuhan, China). Live/dead staining kits, sodium citrate buffer and streptozotocin were purchased from Solarbio (Beijing, China). The activated partial thromboplastin time assay kit and prothrombin time assay kit were purchased from Rayto (Shenzhen, China). The 5-ethynyl-2’-deoxyuridine (EdU) kit was provided by Beyotime (Shanghai, China). The MMP-9 protein reagent for the in vitro cell assay was purchased from Novoprotein (Suzhou, China). The transforming growth factor-β (TGF-β) antibody was provided by Boster (Wuhan, China). The vascular endothelial growth factor (VEGF) antibody and epidermal growth factor (EGF) antibody were purchased from ABclonal (Wuhan, China). The MMP-9 antibody was purchased from Proteintech (Wuhan, China). The platelet endothelial cell adhesion molecule-1 (CD31) antibody was provided by Abcam (Shanghai, China).

Wang Z., Sun L., Wang W., Wang Z., Shi G., Dai H, & Yu A. (2024). A double-network porous hydrogel based on high internal phase emulsions as a vehicle for potassium sucrose octasulfate delivery accelerates diabetic wound healing. Regenerative Biomaterials, 11, rbae024.

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Synthesis of Seawater-Soluble Polymers

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Acrylamide (AM, 99%), acrylic(AA, 98%), N-vinyl pyrrolidone (NVP, 98%), 2-Acrylamide-2-methylpropanesulfonic acid (AMPS, industrial grade), pentaerythritol triallyl ether (PTE, 99%), cetyl dimethylallyl ammonium chloride (C₁₆DMAAC, 80%), potassium formate (HCOOK, industrial grade), sodium hydroxide (NaOH, AR), sodium carbonate (Na₂CO₃, AR), potassium persulfate (KPS, AR), sodium bisulfite (NaHSO₃, AR), all the above materials were purchased from Shanghai Macklin Biochemical Co. LTD, China and were used without any further purification. Seawater is prepared by artificial seawater as Table 1.Table 1

Artificial seawater formulation.

Substances	Concentration (g/L)
NaCl	21.86 ± 0.01
Na₂SO₄	3.23 ± 0.01
MgCl₂	4.53 ± 0.01
CaCl₂	0.93 ± 0.01
KCl	0.64 ± 0.01
NaHCO₃	0.17 ± 0.01
Na₂CO₃	0.02 ± 0.01

Wang C, & Ding W. (2021). Fabrication of a state of the art mesh lock polymer for water based solid free drilling fluid. Scientific Reports, 11, 18870.

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Synthesis of Hierarchical Polymer Electrolyte

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The hierarchical polymer electrolyte (HPE) was synthesized by in situ free radical polymerization. Detailedly, 7.27 g zinc trifluoromethanesulfonate (Zn(CF₃SO₃)₂, 98%, Macklin) was fully dissolved into 20 mL DI water at room temperature. Then, 2 g gelatin (photographic grade, Aladdin) and 30 mg potassium persulfate (KPS) were added in turn to the solution, which were stirred slowly at 80 °C to accelerate dissolution and avoid bubbles. After cooling down to 40 °C, 3 g acrylamide (AM, AR grade, Macklin) and 3.5 mg N,N′-methylenebisacrylamide (BIS, CP grade, Aladdin) were added into the mixture solution ordinally [14 (link)]. After stirring 2 h for grafting reaction, the above solution was injected into a mold with a tiled polyester membrane, which was transferred into an oven at 60 °C for 2–3 h. Finally, a translucent 1 M Zn(CF₃SO₃)₂ HPE with grafted structure was obtained. As a contrast, gelatin electrolyte (GE) film was obtained by the same way except for the addition of acrylamide and N,N′-methylenebisacrylamide.

Chen Z., Wang P., Ji Z., Wang H., Liu J., Wang J., Hu M, & Huang Y. (2020). High-Voltage Flexible Aqueous Zn-Ion Battery with Extremely Low Dropout Voltage and Super-Flat Platform. Nano-Micro Letters, 12, 75.

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Doxorubicin Nanoparticles for Cancer Treatment

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Doxorubicin hydrochloride (DOX) was purchased from J&K Scientific Technology Ltd. Co. 1,3,5-Trimethylbenzene (TMB) and acrylamide (AAm) were purchased from Macklin Reagent (Shanghai, China). Acrylonitrile (AN) and Pluronic® F-127 were purchased from Aladdin Industrial Inc (Shanghai, China). 4-Cyano-4-((phenylcarbonothioyl) thio) pentanoic acid (CPTD), 4’, 6-diamidino-2-phenylindole dihydrochloride (DAPI) were obtained from Sigma-Aldrich (Missouri, USA). Annexin V-FITC Apoptosis Detection Kit was provided by KeChuang Biotechnology Inc (Suzhou, China). All other reagents and solvents were purchased from Sinopharm Chemical Reagent Inc. (Shanghai, China).

Niu X., Fu Y., Feng L., Xie M., Li B., Que L, & You Z. (2023). Upper-critical solution temperature (UCST) polymer functionalized nanomedicine for controlled drug release and hypoxia alleviation in hepatocellular carcinoma therapy. PLOS ONE, 18(8), e0290237.

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Synthesis of Polymeric Materials

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All chemicals, including sulfur powder (Sinoreagent), CuSO₄ (Sinoreagent), KOH (Aladdin), ZnSO₄ (Sinoreagent), thiourea (Sinoreagent), acrylamide (Macklin), potassium persulfate (Sinoreagent), and N, N′-methylene bisacrylamide (Macklin), were used without further purification.

Lin X., Zhang J., Yan H., Xu J., Miao Z., Shu G., Zhao S., Zhang T., Yu H., Yan L., Zhang L, & Shu J. (2023). A triple-synergistic small-molecule sulfur cathode promises energetic Cu-S electrochemistry. Proceedings of the National Academy of Sciences of the United States of America, 120(42), e2312091120.

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Biomimetic Hydrogel Synthesis and Characterization

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Polycaprolactone diol (PCL, 99%), PolyHexamethylene diisocyanate (PHMD, 99%), Hexamethylene diisocyanate (HDI, 99%), Dibutyltin dilaurate (DBTDL, 99%), butyl ethanoate (99%), Acrylamide (AAm, 96%), methacrylic acid (AA, 98%), N,N′-Methylenebis(Acrylamide) (BIS, 99%), Ammonium persulfate (APS), tetramethylethylenediamine (TEMED, 99%) and Ammonium peroxodisulfate (APS, 98%) were purchased from Macklin Inc., Rochelle, IL, USA. All the chemical reagents were used as received.

Peng S., Cao X., Sun Y., Chen L., Ma C., Yang L., Zhao H., Liu Q., Liu Z, & Ma C. (2023). Polyurethane Shape Memory Polymer/pH-Responsive Hydrogel Hybrid for Bi-Function Synergistic Actuations. Gels, 9(5), 428.

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Synthesis of Alginate-based Hydrogel

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Sodium alginate (SA, viscosity (10 g L⁻¹, 20 °C) >0.02 Pa S) was purchased from Sinopharm Group Reagent Co., Ltd; acrylamide (AM, 99.0%), stearyl methacrylate (SMA, 96%) and ammonium persulfate (APS, >99%) were provided by Shanghai Macklin Biochemical Co., Ltd; sodium dodecyl sulfate (SDS, CP) was obtained from Shantou Guanghua Chemical Factory (Guangdong, China); calcium chloride (CaCl₂, AR) was purchased from Guangzhou Chemical Reagent Factory (Guangdong, China); all other reagents used were of analytical grade.

Jing Z., Dai X., Xian X., Du X., Liao M., Hong P, & Li Y. (2020). Tough, stretchable and compressive alginate-based hydrogels achieved by non-covalent interactions. RSC Advances, 10(40), 23592-23606.

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