Bp2401

Manufactured by Thermo Fisher Scientific

The BP2401 is a benchtop pH meter designed for accurate pH measurements in laboratory settings. It features a digital display, automatic temperature compensation, and supports a range of pH electrodes. The core function of the BP2401 is to provide reliable pH measurement capabilities for researchers and laboratory professionals.

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Lab products found in correlation

Protein a g ultralink resin beads, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

BP2401-500

2 protocols using bp2401

HLA-I Peptide Elution and Identification

Cited 1 time

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Peptides were eluted from immunoprecipitated HLA-I molecules as described previously (82 (link)). In brief, transduced or untransduced H1975 cell lines were expanded in 10 cm plates (Corning, 430599), lysed using Triton X-100 (Sigma Aldrich, T9284), and incubated overnight at 4°C under gentle agitation with 50 μg of the pan HLA-A, B, C-specific antibody W6/32 for every 10 mg of protein. HLA class I molecules were immunoprecipitated by using Protein A/G UltraLink resin beads (ThermoFisher Scientific, 53133) and then directly eluted on columns (ThermoFisher Scientific, 89897) using 0.1 N acetic acid (Fisher Scientific, BP2401). HLA-I purification was confirmed by Western blot and eluted fractions were pooled prior to LC-MS/MS analysis.

Jackson K.R., Antunes D.A., Talukder A.H., Maleki A.R., Amagai K., Salmon A., Katailiha A.S., Chiu Y., Fasoulis R., Rigo M.M., Abella J.R., Melendez B.D., Li F., Sun Y., Sonnemann H.M., Belousov V., Frenkel F., Justesen S., Makaju A., Liu Y., Horn D., Lopez-Ferrer D., Huhmer A.F., Hwu P., Roszik J., Hawke D., Kavraki L.E, & Lizée G. (2022). Charge-based interactions through peptide position 4 drive diversity of antigen presentation by human leukocyte antigen class I molecules. PNAS Nexus, 1(3), pgac124.

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Preparation of Rat-Tail Collagen I Hydrogels

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For all assessments, except qualitative gelation, rat-tail collagen I hydrogels (Corning 354249) served as controls. Collagen I hydrogels were prepared at concentrations analogous to iPN hydrogel final concentrations (6.75 and 9 mg ECM per mL solution after neutralization and stabilization) based on the stock collagen I concentration provided by the manufacturer. Collagen I hydrogels were prepared by mixing rat-tail collagen I with a DMEM/HEPES solution consisting of 23.25 mg/mL of sodium bicarbonate (JT Baker 3509-01) in 10X DMEM (Sigma-Aldrich D2429) and HEPES buffer (Fisher SH3085101) mixed 1:1 to form 5X DMEM/HEPES. Final collagen pre-gel solutions always contained 20% DMEM/HEPES solution and 80% collagen I solution. The collagen I was diluted to the appropriate concentration with 0.02% acetic acid (Fisher BP2401), as this was the solvent the collagen was received in from the manufacturer. When using collagen I hydrogels for cell culture experiments, all parts of the fabrication process were performed inside a BSC with sterile materials.

Bousalis D., McCrary M.W., Vaughn N., Hlavac N., Evering A., Kolli S., Song Y.H., Morley C., Angelini T, & Schmidt C.E. (2021). Decellularized Peripheral Nerve as an Injectable Delivery Vehicle for Neural Applications. Journal of biomedical materials research. Part A, 110(3), 595-611.

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