Sw837

Colorectal cancer cell lines DLD-1, RKO and SW837 were obtained from the American Type Culture Collection (Manassas, VA, USA). DLD-1 and SW837 cells were cultured in RPMI1640 medium and RKO cells were cultured in DMEM/F-12 medium, both supplemented with antibiotics and 10% foetal bovine serum (Invitrogen, Carlsbad, CA, USA) at 37 °C in 5% CO₂. Near-diploid (2N) and near-tetraploid (4N) clones of DLD-1 and RKO cell lines were previously generated in our laboratory^{16 (link)}. Cytokinesis blockage with 1.5 μg/ml of dihydrocytochalasin B (Sigma-Aldrich, St. Louis, MO, USA) for 24 h was performed in double thymidine treated SW837 to establish 4N clones. Wild-type and post-tetraploid clones derived from the hTERT-immortalized retinal-pigmented epithelial cells (RPE1) (kindly provided by Z. Storchova, University of Kaiserslautern, Kaiserslautern, Germany) were cultured in DMEM/F-12 medium supplemented with antibiotics and 10% FBS at 37 °C in 5% CO₂. For the experiments described in this study, we used one 2N clone and two 4N clones derived from the DLD-1, RKO and SW837 cell lines. As for the RPE1 cells, we used one 2N and one 4N clone.