Lambda 650 spectrometer

Manufactured by PerkinElmer

The Lambda 650 spectrometer is a UV-Vis spectrophotometer designed for routine analysis. It measures the absorbance or transmission of light through a sample across the ultraviolet and visible wavelength ranges.

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Lab products found in correlation

Jem 2100, by JEOL (2 mentions) Sta 409 pc luxx, by Netzsch (1 mentions) 2400 chn elemental analyzer, by PerkinElmer (1 mentions) Ptp 6 peltier system, by PerkinElmer (1 mentions) Hct ion trap, by Bruker (1 mentions) Fluoromax 4 spectrofluorimeter, by Horiba (1 mentions) Fluorolog 3 spectrofluorometer, by Horiba (1 mentions) Regulus 8100, by Thermo Fisher Scientific (1 mentions) Fei tecnai g2 f20, by Thermo Fisher Scientific (1 mentions) Nicolet is50 ftir spectrometer, by Thermo Fisher Scientific (1 mentions) D8 advanced diffractometer, by Bruker (1 mentions)

Close spelling variants of this product

Lambda 650S UV-vis spectrometer Lambda 650S spectrometer Lambda 650 UV/VIS Spectrometer Lambda 650 Spectrometers

9 protocols using lambda 650 spectrometer

Characterization of Au Nanoparticles

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The obtained nanostructures were examined by transmission electron microscopy (TEM) using a JEOL JEM-2100 (JEOL GmbH, Eching, Germany). The size distributions of Au nanoparticles were determined from TEM images using the ImageJ software [39 (link)]. More than 100 Au nanoparticles were counted. For the measurement of UV–Vis absorption spectra, the reaction solution with Au@PDA particles as catalyst was placed in a quartz sample cell with a 1.0-cm cell path length. UV–Vis spectra (at 400 nm) were recorded by using Lambda 650 spectrometer supplied by PerkinElmer at 20 °C with reference spectrum of the Au@PDA particles in water. TGA measurements were taken using a Netzsch STA409PC LUXX from 25 to 600 °C under a constant argon flow (30 mL min⁻¹) with a heating rate of 10 K/min. Nitrogen adsorption experiments were performed with a Quantachrome Autosorb-1 at liquid nitrogen temperature, and data analysis was performed by Quantachrome software. The specific surface area was calculated using the Brunauer–Emmett–Teller (BET) equation. Pore size distribution was determined by Barrett–Joyner–Halenda (BJH) method. Samples were degassed at 50 °C for 24 h before measurements.

Mei S., Kochovski Z., Roa R., Gu S., Xu X., Yu H., Dzubiella J., Ballauff M, & Lu Y. (2019). Enhanced Catalytic Activity of Gold@Polydopamine Nanoreactors with Multi-compartment Structure Under NIR Irradiation. Nano-Micro Letters, 11, 83.

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Nanowire Morphology Analysis by TEM

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The morphology of the nanowires was investigated by transmission electron microscopy (TEM) using a JEOL JEM-2100 (JEOL GmbH, Eching, Germany) at an acceleration voltage of 200 kV. Samples were prepared by placing a drop of the diluted nanowires solution on the carbon-coated copper grids and dried under ambient temperature. The ultraviolet-visible spectra (UV-VIS spectra) were measured by Lambda 650 spectrometer supplied by Perkin-Elmer or Agilent 8453.

Rothe M., Zhao Y., Kewes G., Kochovski Z., Sigle W., van Aken P.A., Koch C., Ballauff M., Lu Y, & Benson O. (2019). Silver nanowires with optimized silica coating as versatile plasmonic resonators. Scientific Reports, 9, 3859.

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UV-Vis Diffuse Reflectance Spectroscopy of Compound 1

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UV-Vis diffuse reflectance spectra of a powdered sample of compound 1 were measured with a Lambda 650 spectrometer (PerkinElmer). The powder was mounted on a glass plate and then covered with a quartz glass plate. In each experiment, the sample was picked up from the measurement holder, and then irradiated by UV light (365 nm, 40 mW cm⁻²) from the quartz glass side. Every measurement was performed in the range 200–800 nm at 1 nm resolution with a scan speed of 200 nm min⁻¹.

Taniguchi T., Kubota A., Moritoki T., Asahi T, & Koshima H. (2018). Two-step photomechanical motion of a dibenzobarrelene crystal. RSC Advances, 8(60), 34314-34320.

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Preparation and Adsorption Study of Li2S8 Solution

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To prepare Li₂S₈ solution for the adsorption test, S and Li₂S power with a molar ratio of 5:1 were dissolved in the mixed solution, DME and DOL with the ratio of 1:1 v/v, and stirred for 48 h at 80°C in the glove box. Then, taking out 4 mL Li₂S₈ solution with a concentration of 1 mM in two sample bottles and adding different amounts of Etched‐Halloysite‐550 and NiO@Halloysite with the same surface area (1.5 m² g⁻¹, calculated from BET‐specific surface area results in Table S1), respectively. Taking pictures and observing the changes in different samples after aging for 5 min and 1 h in the glove box, the UV–vis spectroscopy (Lambda 650 spectrometer, PerkinElmer) test was performed to the supernatant solutions.

Elibol M.K., Jiang L., Xie D., Cao S., Pan X., Härk E, & Lu Y. (2023). Nickel Oxide Decorated Halloysite Nanotubes as Sulfur Host Materials for Lithium–Sulfur Batteries. Global Challenges, 7(7), 2300005.

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Elemental and Spectroscopic Analysis of Compounds

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Elemental analyses were performed by the microanalytical service of the Faculty of Chemistry of the University of Vienna on a Perkin–Elmer 2400 CHN Elemental Analyzer. UV/Vis spectra were recorded at 25 °C using a Perkin–Elmer Lambda 650 spectrometer equipped with an optical cell of 1 cm path-length in the wavelength range of 200 to 800 nm in combination with a Perkin–Elmer PTP-6 Peltier System. Electrospray ionization (ESI) mass spectrometry measurements were conducted on a Bruker HCT ion trap (Bruker Daltonics GmbH) by using methanol as a solvent. MIR spectra were recorded on a Perkin–Elmer 370 FTIR 2000 instrument using an ATR (attenuated total reflection) unit in the range of 4000–400 cm⁻¹. Phosphorescence emission spectra were recorded with a Horiba FluoroMax-4 spectrofluorimeter and the data were processed using the FluorEssence v3.5 software package.

Kuhn P.S., Cremer L., Gavriluta A., Jovanović K.K., Filipović L., Hummer A.A., Büchel G.E., Dojčinović B.P., Meier S.M., Rompel A., Radulović S., Tommasino J.B., Luneau D, & Arion V.B. (2015). Heteropentanuclear Oxalato-Bridged nd–4f (n=4, 5) Metal Complexes with NO Ligand: Synthesis, Crystal Structures, Aqueous Stability and Antiproliferative Activity. Chemistry (Weinheim an Der Bergstrasse, Germany), 21(39), 13703-13713.

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Characterization of Photochromic Liquid Crystals

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All chemicals and solvents were purchased from commercial suppliers and used without further purification. ¹H NMR, ¹³C NMR and ¹⁹F NMR spectra were recorded in CDCl₃. Chemical shifts are in δ units (ppm) with the residual solvent peak as the internal standard. The coupling constant (J) is reported in hertz (Hz). NMR splitting patterns are designed as follows: s, singlet; d, doublet; t, triplet; and m, multiplet. UV-vis spectrum was taken by a Perkin Elmer Lambda 650 Spectrometer. Textures, contact areas, reflection colors and distance changes of disclination lines were observed by a Leika DM2500p polarizing optical microscopy (POM). 530 nm light irradiation was carried out by a CCS HLV-24GR-3W LED light source. 445 nm light irradiation was carried out by a JD BL-445-300 laser light source. Reflection spectra of CLCs were examined with an Ideaoptica spectrometer.

Qin L., Gu W., Chen Y., Wei J, & Yu Y. (2018). Efficient visible-light full-color tuning of self-organized helical superstructures enabled by fluorinated chiral switches. RSC Advances, 8(68), 38935-38940.

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Optical Characterization of Dye-Doped Polymers

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Absorption spectra were recorded at room temperature on a Perkin-Elmer Lambda 650 spectrometer. Fluorescence spectra were measured at room temperature on a Horiba Jobin-Yvon Fluorolog®-3 spectrofluorometer and equipped with a 450 W xenon arc lamp, double-grating excitation and single-grating emission monochromators. The fluorescence of dye/PMMA films were recorded by using the Solid-Sample Holder and collecting the front-face emission at 30°.
The fluorescence quantum yield (Φ_f) in tetrahydrofuran (THF) was determined at room temperature relative to quinine sulphate (

Φ_{f}^{S}

= 0.54 in 0.1 M H₂SO₄) using the following relation:

Φ_{x} = Φ_{S T} (\frac{{Grad}_{X}}{{Grad}_{ST}}) (\frac{η_{X}^{2}}{η_{ST}^{2}})

Where the subscripts ST and X are standard and dye respectively, “Grad” is the gradient from the plot of integrated fluorescence intensity versus absorbance for different solutions of standard and dyes. In order to minimise re-absorption effects concentration was kept low, so absorbances never exceed 0.1 at and above the excitation wavelength. η is the refractive index of the solvent, i.e. 1.405 for THF and 1.333 for water39 . A thin film of about 10⁻³ M 9,10-diphenylanthracene in PMMA was used as a standard (

Φ_{f}^{S}

= 0.83)40 for the quantum yield measurement of dye/PMMA films.

Barone V., Bellina F., Biczysko M., Bloino J., Fornaro T., Latouche C., Lessi M., Marianetti G., Minei P., Panattoni A, & Pucci A. (2015). Toward the Design of Alkynylimidazole Fluorophores: Computational and Experimental Characterization of Spectroscopic Features in Solution and in Poly(methyl methacrylate). Physical chemistry chemical physics : PCCP, 17(40), 26710-26723.

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Comprehensive Characterization of MgAl2O4 Nanomaterials

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The crystalline
phase of the as-synthesized products and the raw material were examined
by a X-ray diffractometer [Rigaku, SmartLab (3KW), Japan] with Cu
target and Ka radiation. The morphological characteristics of MgAl₂O₄ powders were characterized by SEM (Hitachi,
Regulus8100, Japan) and transmission electron microscopy (FEI, FEI
Tecnai G2 F20, America). Energy-dispersive X-ray spectroscopy (EDAX
Octane Elect, America) was used to observe the elemental distribution
of the samples. Fourier transform infrared spectra (FT-IR) of the
samples were recorded on a Nicolet iS50 FT-IR spectrometer (Thermo,
America) in the scan range of 4000 to 400 cm^–1.
UV–vis diffused reflectance spectra (DRS) of samples were registered
on a Lambda 650 spectrometer (PerkinElmer, America) in the UV–vis
region of 200–800 nm. The photoluminescence (PL) measurements
were performed on an iHR320 spectrometer (Edinburgh Instruments, England)
at room temperature. The particle size was obtained from Mastersizer
2000 (Malvern, England).
Differential scanning calorimetry (DSC)
and thermogravimetric (TG) analysis (Mettler Toledo, Columbus, OH)
were used to determine the reactions of the alloy powder in air or
oxygen. Approximately 2.0 mg of the sample was heated at a rate of
20 °C/min from 50 to 1100 °C in a ceramic crucible. The
flow rate of the air or oxygen was kept at 50 mL/min.

Wang Y.K., Xie X, & Zhu C.G. (2022). Self-Propagating High-Temperature Synthesis of Magnesium Aluminate Spinel Using Mg–Al Alloy. ACS Omega, 7(15), 12617-12623.

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Hydrothermal Synthesis of VF3-Bi2O3 Crystals

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Hydrothermal synthesis: Single crystals were prepared through the hydrothermal route. A mixture of VF3 (1mmol) and Bi2O3 (1 mmol) were added together with 2.5 ml of deionized water and 0.5 ml of HF solution (7.65%) in a 23 ml Teflon lined steel autoclave. Mixture was heated at 230 °C for 72 h and then cooled to room temperature. Products were successively washed with deionized water and ethanol and left to dry in air at room temperature. Black crystals were isolated for characterizations. XRD: Powder XRD was carried out on a Bruker D8 Advanced diffractometer working in Bragg-Brentano geometry and using Cu radiation. The single crystal data collected on a Bruker X8 diffractometer (Ag K radiation ) have treated using the Jana 2006 suite. 51 The powder data have been analyzed using the FULLPROF suite. 52 (link) Magnetic measurement: Magnetic data and heat capacities were measured on a Physical Property Measurement System Quantum Design PPMS DynaCool system, using BiVO3F ground powder. UV visible spectroscopy: It was measured on a Perkin Elmer Lambda 650 Spectrometer. The bandgap was calculated from the calculation of the Kubelka-Munk 𝐹(R)=(1-𝑅 2 )2𝑅 function and using Tauc plot (F(R) *R *E 1/2 ) versus E, assuming direct allowed transitions.

Mentré O., Juárez-Rosete M.A., Saitzek S., Aguilar-Maldonado C., Colmont M, & Arévalo-López Á.M. (2021). S = 1/2 Chain in BiVO₃F: Spin Dimers versus Photoanodic Properties. Journal of the American Chemical Society, 143(18).

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