Participants were examined after an overnight fast. Each participant received a 75 g oral fructose and had serum samples collected at 60 minutes and 120 minutes for testing of serum uric acid and other laboratory parameters (glucose, LDL cholesterol, HDL cholesterol and VLDL cholesterol). Serum uric acid was measured by autoanalyzer (Siemens Dimension RXL Max), using the uricase method.
Rxl max
Manufactured by Siemens
Sourced in Germany
The RxL Max is a versatile lab equipment product offered by Siemens. It is designed to perform a range of laboratory tasks. The specific core function of the RxL Max is to provide accurate and reliable analytical capabilities for various applications. Further details on the intended use of this product are not available.
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Lab products found in correlation
8 protocols using rxl max
1
Fructose Challenge and Metabolic Effects
2
Comprehensive Laboratory Assessments for Clinical Management
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The following laboratory parameters (among others) were assessed using standard methods and were immediately available for treating physicians:
haematology: haemoglobin, haematocrit, leukocytes (white blood cell), and platelets;
serum electrolytes: sodium (Na+) and potassium (K+);
renal and liver function tests: creatinine, estimated glomerular filtration rate (eGFR) by Modification of Diet in Renal Disease method was calculated,
plasma N‐terminal pro‐B‐type natriuretic peptide (NT‐proBNP) (method: immunoenzymatic, Siemens, Marburg, Germany) and troponin I (method: immunoenzymatic, single Dimension RxL Max, Siemens).
3
Comprehensive Cardiovascular Biomarker Profiling
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Laboratory parameters were assessed using the standard methods in our laboratory, including plasma NTproBNP (N-Terminal Pro-B-Type Natriuretic Peptide) (method: immunoenzymatic, Siemens, Marburg, Germany) and troponin (TNI) (method: immunoenzymatic, one-dimensional RxLMax, Siemens). The serum sTfR (mg/L) was measured from plasma frozen at −70 °C using immunonephelometry (Siemens Healthcare Diagnostics, Inc., Deerfield, IL, USA).
The Quantikine ELISA Immunoassay kit (R&D Systems, Inc., Minneapolis, MN, USA) was used to determine the levels of the remaining markers of interest. This assay employs the quantitative sandwich enzyme immunoassay technique. These were the following proteins: GDF-15 (also known as macrophage inhibitory cytokine-1 (MIC-1) (n = 79), CXL4/PF4 (Platelet Factor 4), follistatin (FS), MMP-9 (matrix metalloproteinases—gelatinase) (n = 159), lipocalin-2 (NGAL) (n = 159), myostatin (GDF-8), E-Selectin (CD62E), ICAM-1 (CD54 allele-specific), Il-6 and Il-22 (n = 159). The Synergy/HTX multi-mode reader analyzer was used for the measurement of absorbance. This research was carried out in the laboratory of the Department of Clinical Pharmacology at Wroclaw Medical University.
The Quantikine ELISA Immunoassay kit (R&D Systems, Inc., Minneapolis, MN, USA) was used to determine the levels of the remaining markers of interest. This assay employs the quantitative sandwich enzyme immunoassay technique. These were the following proteins: GDF-15 (also known as macrophage inhibitory cytokine-1 (MIC-1) (n = 79), CXL4/PF4 (Platelet Factor 4), follistatin (FS), MMP-9 (matrix metalloproteinases—gelatinase) (n = 159), lipocalin-2 (NGAL) (n = 159), myostatin (GDF-8), E-Selectin (CD62E), ICAM-1 (CD54 allele-specific), Il-6 and Il-22 (n = 159). The Synergy/HTX multi-mode reader analyzer was used for the measurement of absorbance. This research was carried out in the laboratory of the Department of Clinical Pharmacology at Wroclaw Medical University.
4
Serum Biomarker Profiling in Cardiac Puncture
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Blood serum was harvested from 200 to 500 μL whole blood obtained via cardiac puncture into a Microvette® 500 Z-Gel tube. Serum levels of urea, albumin, aspartate aminotransferase and alanine aminotransaminase were determined using a Siemens Dimension® RxL Max integrated chemistry system. Proteinuria was assessed by running 5 µl of fresh urine into a 4–12% precast SDS-PAGE gel and staining with Coomassie. Full uncropped gel can be found in Supplementary Fig. 10 .
5
Serum Biomarker Quantification
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Serum samples were stored at −80°C. Serum creatinine (SCr) and alanine aminotransferase (ALT) levels were determined by spectrophotometric methods using an autoanalyzer (RxL-Max, Siemens, Munich, Germany).
6
Thyroid and Glucose Monitoring in Pregnancy
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Thyroid stimulating hormone (TSH), free T4, and random blood sugar were done at baseline, and free T4 and TSH were repeated in each trimester. Total T4, T3, HbA1c, and free T3 were recorded if available. All the tests were done with an automated analyzer (Siemens Dimension, RxL Max. United States of America). All the hormonal assays were done by chemiluminescence method. Blood glucose was measured by glucose oxidase method.
7
Urine Biomarkers in Heart Failure
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The laboratory parameters were assessed using standard methods in our laboratory including plasma NT-proBNP (N-Terminal Pro-B-Type Natriuretic Peptide) (method:immunoenzymatic, Siemens, Marburg, Germany), troponin (TNI) (method: immunoenzymatic, single Dimension RxLMax, Siemens).
We designed the first, baseline, urine sample to be obtained before the first in-hospital dose of intravenous furosemide. However, if the patient was not able to provide this sample before iv furosemide, the first available sample was taken. At day-1, day-2 and at discharge the first post diuretic urine was collected. The more detailed methods regarding urine sampling and assessment were presented elsewhere [11] (link).
All patients were instructed to limit their fluid intake to 1.5-2 L per 24 h as well as they were advised to limit their daily sodium intake during hospitalization as part of routine clinical practice in our institution.
We designed the first, baseline, urine sample to be obtained before the first in-hospital dose of intravenous furosemide. However, if the patient was not able to provide this sample before iv furosemide, the first available sample was taken. At day-1, day-2 and at discharge the first post diuretic urine was collected. The more detailed methods regarding urine sampling and assessment were presented elsewhere [11] (link).
All patients were instructed to limit their fluid intake to 1.5-2 L per 24 h as well as they were advised to limit their daily sodium intake during hospitalization as part of routine clinical practice in our institution.
8
Serum Sodium and EGFR Mutation Detection
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Serum sodium levels were measured by an indirect ion-selective electrodes method using fully automated Siemens Dimension ® Rxl Max ® integrated chemistry system. Pretreatment sodium level (<7 days before starting chemotherapy) was obtained from EMR. Patients with serum sodium ≥136 mEq/L at baseline were categorized as normal serum sodium (NSS) and those with serum sodium <136 mEq/L were considered as LSS. Management of LSS consisted of fluid restriction, sodium tablets, or hypertonic saline as per standard guidelines depending on the severity. [ 10 ] Epidermal growth factor receptor mutation EGFR mutation detection was carried out using TaqMan-based real-time polymerase chain reaction technique as described previously.
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