Alexa488 conjugated anti rabbit

Manufactured by Thermo Fisher Scientific

Sourced in United States

Alexa488-conjugated anti-rabbit is a fluorescently labeled secondary antibody that specifically binds to rabbit primary antibodies. The Alexa488 fluorescent dye allows for the detection and visualization of target proteins in various applications such as immunofluorescence, flow cytometry, and Western blotting.

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Lab products found in correlation

Cy3 conjugated anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Gfap mab360, by Merck Group (1 mentions) Alexa488 conjugated anti mouse, by Jackson ImmunoResearch (1 mentions) Lsm 510, by Zeiss (1 mentions) Mouse anti p65, by Cell Signaling Technology (1 mentions) Anti mouse igg antibody, by Thermo Fisher Scientific (1 mentions) Rabbit anti mmp 9 antibody, by Abcam (1 mentions) Alexa 594, by Thermo Fisher Scientific (1 mentions) Ab75852, by Abcam (1 mentions) β actin, by Merck Group (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Merck Group (1 mentions) Ab52649, by Abcam (1 mentions)

Close spelling variants of this product

Anti-rabbit Alexa488 (45 citations) Alexa488-conjugated goat anti-rabbit Ig’ (4 citations) Alexa488 conjugated rabbit anti-mouse antibody (4 citations) Alexa488- or Alexa594-conjugated anti-mouse or anti-rabbit antibodies (2 citations) Goat anti-rabbit Alexa488-conjugated secondary antibody (2 citations) Goat anti-rabbit conjugated with Alexa488 (2 citations) Alexa488 conjugated rabbit anti-GFP antibody (2 citations) Alexa488-conjugated goat anti-rabbit IgG secondary antibodies (2 citations)

5 protocols using alexa488 conjugated anti rabbit

Multimodal Protein Marker Detection

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We used the antibodies against the following proteins: TSPO (ab109497), AIF1/IBA1 (ab5076), MAP2 (ab5392), and COX4I1 (Ab14744) from Abcam; VIM/vimentin (AB5733), RBFOX3/NeuN (MAB377), and GFAP (MAB360) from Millipore; NES/nestin (NB100-1604) and NEFM/NFM (NBP2-46,618) from Novus Biologicals; LAMP2 (L0668), MAP1LC3B/LC3B (L8918), SQSTM1 (P0067), and ATG7 (A2856) from Sigma-Aldrich; AGRP (AF634) from R&D Systems; p-PRKAA/p-AMPKα (Thr172, 2535), PRKAA/AMPKα (2532), p-ACAC (Ser79, 3661), ACAC (3662), p-ULK1 (Ser555, 5869), ULK1 (8054), BECN1/Beclin1 (3738), and GAPDH (2118) from Cell Signaling Technology
The following secondary antibodies were used: Cy3-conjugated anti-rabbit IgG (Jackson ImmunoResearch, 711–165-152), Alexa488-conjugated anti-rabbit (Thermo Fisher Scientific, A-11,008), Alexa488-conjugated anti-mouse (Jackson ImmunoResearch, 715–545-150), Alexa488-conjugated anti-goat (Molecular Probes, A-11,055), Alexa488-conjugated anti-chicken IgG (Jackson ImmunoResearch, 703–545-155). Alexa647-conjugated anti-chicken IgG (Jackson ImmunoResearch, 703–545-155), and horse radish peroxidase-conjugated anti-rabbit (Thermo Fisher Scientific, NCI460KR) and anti-mouse (Cell Signaling Technology, 7076) antibodies.

Kim S., Kim N., Park S., Jeon Y., Lee J., Yoo S.J., Lee J.W., Moon C., Yu S.W, & Kim E.K. (2019). Tanycytic TSPO inhibition induces lipophagy to regulate lipid metabolism and improve energy balance. Autophagy, 16(7), 1200-1220.

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Subcellular PCDH19 Localization in Cell Lines

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5 × 10⁴ HeLa cells, 1.5 × 10⁵ MDCK cells or 0.5 × 10⁵ mouse primary hippocampal neurons (E18.5) were plated per well of a 12-well culture dish onto poly-L-lysine coated coverslips and transfected with either full-length or C-terminal Myc-PCDH19 expression constructs. Next day, the cells were fixed in 4% paraformaldehyde/1× PBS for 10 min, permeabilized with 0.2% Triton-X-100/1× PBS for 5 min, blocked in 10% horse serum/1× PBS for 1h and then incubated with either anti-Myc, anti-NONO or both antibodies. Primary antibodies were visualized with alexa-488 conjugated anti-rabbit (for anti-Myc) or alexa-555 conjugated anti-mouse IgG (for anti-NONO) (Thermo Scientific). All images were acquired at either 20× or 60× magnification using AxioVision Rel. 4.8 (Carl Zeiss).

Pham D.H., Tan C.C., Homan C.C., Kolc K.L., Corbett M.A., McAninch D., Fox A.H., Thomas P.Q., Kumar R, & Gecz J. (2017). Protocadherin 19 (PCDH19) interacts with paraspeckle protein NONO to co-regulate gene expression with estrogen receptor alpha (ERα). Human Molecular Genetics, 26(11), 2042-2052.

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Immunohistochemistry of MMP9 and p65 in LX-2 cells and liver tissues

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For MMP9 and p65 immunostaining, LX-2 cells were fixed with 4% paraformaldehyde for 2 h at room temperature. Antigens were retrieved by incubation in the 10 mM citrate buffer for 20 min. The cells were then incubated with rabbit anti-MMP-9 antibody (1:200, Abcam) or mouse anti-p65 (1:200, Cell Signaling Technology). The specifically bound primary antibodies were detected with Alexa 488-conjugated antirabbit or anti-mouse IgG antibodies (Thermo Fisher Scientific), respectively. The unbound antibodies were rinsed off by PBS. After counterstaining with DAPI for the nucleus, the images were captured with a confocal microscope (Zeiss LSM 510). For liver tissues, the sections were rehydrated and incubated with anti-neutrophil elastase (NE) (1:500) antibody overnight as described previously (Xin et al., 2007 (link)). Specifically bound antibodies visualized with the immunohistochemistry kit (MX Biotechnologies, Fuzhou, CN) according to the manufacturer’s protocol. Hematoxylin was used for counterstaining.

Wang C., Li Y., Li H., Zhang Y., Ying Z., Wang X., Zhang T., Zhang W., Fan Z., Li X., Ma J, & Pan X. (2020). Disruption of FGF Signaling Ameliorates Inflammatory Response in Hepatic Stellate Cells. Frontiers in Cell and Developmental Biology, 8, 601.

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Doublecortin Expression in Mouse Brain

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Mice received i.p. administration of YL or vehicle, then brains were removed 24 hours later. For immunohistochemistry, brains were fixed with 4% PFA and then immunostained with an anti-doublecortin (Dcx) (1:1000; rabbit-IgG; Abcam) antibody. Alexa 488 conjugated anti-rabbit (1:1000; Thermo Fisher Scientific Inc.) was used as the secondary antibody. Dcx immunostaining intensity was measured using Image J software.

Mizushige T., Uchida T, & Ohinata K. (2020). Dipeptide tyrosyl-leucine exhibits antidepressant-like activity in mice. Scientific Reports, 10, 2257.

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Protocol for Biochemical Analysis of BEST1 and Cathepsin D

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CHX, chloroquine phosphate, lactacystin, MG132, 3-MA, calpain inhibitor I (ALLN), 4-PBA and TG (Biomol, Hamburg, Germany) were obtained commercially. Substances were prepared as stock solutions in dimethyl sulfoxide: lactacystin (10 mM), MG132 (40 mM), ALLN (5 mg/ml) and TG (2.5 mM).
Rabbit polyclonal antibody hBEST1–334 was described previously (9 (link)), and used in 1:2500 (WB) or 1:250 (ICC) dilution. Rabbit polyclonal antibody ctsD (ab75852, Abcam, Cambridge, UK; dilution 1: 1500), mouse monoclonal antibodies β-actin (no. 5441, Sigma-Aldrich, Munich, Germany; dilution 1:10 000) and GM130 (ab 52649, Abcam, Cambridge, UK; dilution 1:1000), are commercially available. Secondary antibodies for immunofluorescence were goat Alexa 594- and Alexa 488-conjugated anti-rabbit (Thermofisher Scientific, Waltham, USA 1:500). Western blot experiments were performed with goat near-infrared fluorescent dyes (IRDye 1:10 000) (Lycor, Bad Homburg, Germany) or horseradish peroxidase-conjugated secondary antibodies (Calbiochem/Merck Millipore, Darmstadt, Germany; 1:10 000).

Milenkovic A., Milenkovic V.M., Wetzel C.H, & Weber B.H. (2018). BEST1 protein stability and degradation pathways differ between autosomal dominant Best disease and autosomal recessive bestrophinopathy accounting for the distinct retinal phenotypes. Human Molecular Genetics, 27(9), 1630-1641.

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