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10 protocols using oleylamine

1

Synthesis of Chondroitin Sulfate Nanoparticles

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Manganese (II) acetate tetrahydrate, 1‐octadecene, oleylamine, oleic acid, sodium hydroxide, chondroitin 4‐sulfate sodium salt (CS), and 1,4‐butanediol diglycidyl ether were purchased from Shanghai Macklin Biochemical Co. Ltd. (Shanghai). Acetone, n‐hexane, and hydrochloric acid were purchased from Sinopharm Chemical Reagent Co. Ltd. All the chemicals were used without further purification.
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2

Synthesis of Lead Halide Perovskite Nanocrystals

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PbBr2 (99.99%), Cs2CO3 (99.9%), oleic acid (OA, AR), oleylamine (OAm, AR) were purchased from Macklin Inc. Hexane, toluene, and ethyl acetate were analytical grade and were purchased from Aladdin Inc. Poly (bis(4-phenyl)(2,4,6-trimethylphenyl) amine) (PTAA), and 1,3,5-Tris(1-phenyl-1H-benzimidazol-2-yl)benzene (TPBi) were purchased from Xi’an Polymer Light Technology Corp. (PLT). All reagents were used as received without further purification.
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3

Synthesis of Colloidal Nanocrystals

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Lead chloride (PbCl2, 99.999%), lead bromide (PbBr2, 99.999%) and cesium carbonate (Cs2CO3, 99.995%) were purchased from Sigma-Aldrich. Oleylamine (OAm, 90%), undec-10-en-1-amine (EAm, 95%), octanoic acid (OAc, 99%), anhydrous p-xylene (99%), methyltrioctylammonium chloride (MTOA, 97%), methyltrioctylammonium bromide (MTOABr, 98%), 4-vinylbenzyl chloride (VBCl, 98%), methyl acetate (MeOAc, 99%), 2,2’-Azobis(2,4-dimethyl)valeronitrile (ABVN, 98%) and styrene (St, 99%) were purchased from Macklin reagent. N,N-dimethyl-4-vinylaniline (DMVA,90%) was purchased from Acros Organics.
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4

Synthesis of Magnetic Nanoparticles

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CNTs, oleylamine, and dibenzyl
ether were purchased from Shanghai Macklin Biochemical Co., Ltd.;
glucose and n-hexane were purchased from Tianjin Kaitong Chemical
Reagent Co., Ltd.; anhydrous ethanol and oleic acid were purchased
from Tianjin Tianli Chemical Reagent Co., Ltd.; iron acetylacetonate
was purchased from Shanghai Rhawn Chemical Technology Co., Ltd.; octadecene
was purchased from Sinopharm Chemical Reagent Co., Ltd. Specifications
for above materials are AR.
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5

Perovskite Solar Cell Fabrication

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Oleic acid (OA, 99%), oleylamine (OAM, 80–90%), 1-octadecene (ODE, > 90%), methyl acetate (MeOAc, 98%) were purchased from Shanghai Macklin Biochemical Co., LTD. Ethanol (AR), isopropanol (AR), acetone (AR), toluene (AR) were purchased from Sinopharm Chemical Reagent Co., LTD. Cesium carbonate (Cs2CO3, 99%) and stannous chloride dihydrate (SnCl2·2H2O, 98%) were purchased from Aladdin Reagent Co., LTD. Lead iodide (PbI2, 99.99%) and methylammonium iodide (MAI, 99.5%) from Greatcell Solar Materials Pty., Ltd. N,N-dimethylformamide (DMF, 99.8%) and dimethyl sulfoxide (DMSO, 99.7%) were purchased from Sigma-Aldrich (Shanghai) Trading Co., LTD. Fluorine-doped tin oxide (FTO) was purchased from YouXuan Technology Co., Ltd. Carbon electrode paste purchased from Shanghai MaterWin New Materials Co., LTD.
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6

Magnetic Nanoparticle-Peptide Conjugates

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Cur was obtained from Shanghai Winherb Medical Technology Co., Ltd. (Shanghai, China). Fe(acac)3, Mn(acac)2, 1,2-hexadecanediol, oleic acid, oleyl amine and dibenzyl ether were obtained from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China). DSPE-PEG-COOH and DSPE-PEG-Mal (both with a PEG molecular weight = 2000) were obtained from Aladdin Reagents (Shanghai, China). Tetrahydrofuran (THF) was obtained from Damao Chemical Reagent Factory (Tianjin, China). Peptides QSH and CRT were obtained from GL Biochem (Shanghai) Ltd. (Shanghai, China). Cell counting Kit-8 was obtained from Beyotine (Shanghai, China). Antibody against 6E10 was obtained from Biolegend (San Diego, CA, USA). Antibody against NLRP3 was obtained from AdipoGen (San Diego, CA, USA). Antibodies against Iba1, IL-18, BDNF, doublecortin (DCX), CD68, GFAP, NeuN and GAPDH were obtained from Abcam (Cambridge, UK). Antibodies against ASC, β-Actin and horseradish peroxide (HRP)-conjugated secondary antibody were obtained from Cell Signaling Technology (Danvers, MA, USA). The chemiluminescent HRP substrate was obtained from Millipore (Billerica, MA, USA). All other reagents were analytical grade or better.
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7

Synthesis and Characterization of Functionalized Nanoparticles

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Bulk BP, N-Methyl pyrrolidone (NMP), ethanol, 1-ethyl-3-[3-(dimethylamino)-propyl] carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), cyclohexane, thiazolyl blue tetrazolium bromide (MTT), propidium iodide (PI) were obtained from Sigma-Aldrich. Oleyl amine, 1-Octadecene, Methoxy Polyethylene Glycol Amine and Poly (acrylic acid) (PAA) (M.W ~ 2000) were obtained from Macklin. FeCl24H2O was obtained from Aladdin.
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8

Synthesis of Cerium Oxide Nanoparticles

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Cerium acetate (CAS: 206,996–60-3, Lot: E1822034, 99.99%) was obtained from Aladdin Industrial Inc. (Shanghai, China). Oleylamine (CAS: 112–90-3, Lot: C14418632, 80–90%) was obtained from Macklin Biochemical Co., Ltd (Shanghai, China). Xylene (CAS: 1330–20-7, Lot: 74,847,021, 99%) was obtained from Meryer Technologies Co., Ltd. (Shanghai, China). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000] (DSPE-mPEG2000 (CAS: 147,867–65-0, Lot: 9JEDMRAQ, ≥ 95%) was purchased from Alab (Shanghai) Chemical Technology Co., Ltd. DSPE-mPEG2000-FITC was obtained from Xi’an Ruixi Biological Technology Co., Ltd. Hydrogen peroxide (H2O2) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shaanxi, China).
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9

Synthesis of Organic Halide Compounds

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Lead bromide (PbBr2, 99%) was purchased from Shanghai Aladdin Bio-Chem Technology Co., Ltd (China). Hydrobromic acid (HBr, 40%), oleic acid (OA, 90%), oleylamine (OAm, 90%) and ethyl acetate (AR) were supplied by Macklin Biochemical Co., Ltd (Shanghai, China). Methylamine aqueous solution (MA, AR), butylamine (BA, AR), citric acid (AR), and N,N-dimethylformamide (DMF, AR) were obtained from Taixin Chemical Industry Co., Ltd (Chongqing, China). The dialysis membrane (2K MWCO) was acquired from Sangon Biotech Co., Ltd (Shanghai, China).
MABr was synthesized by stirring 5 mL of MA, 5 mL of HBr and 10 mL of ethanol in an ice bath for 2 h. Then the solution was heated at 60 °C to obtain the crude MABr. The white powder was washed with ethyl ether several times, and then dried in a vacuum oven at 45 °C to acquire the purified MABr. Meanwhile, BABr was synthesized using the same method as described above.
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10

Synthesis of Cesium-Lead-Cerium Halide Perovskite Nanocrystals

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Caesium carbonate (Cs2CO3, 99.9%), oleic acid (OA, technical grade 90%) were purchased from Sigma‐Aldrich. Lead(II) bromide (PbBr2, 99.998%), cerium(III) bromide (CeBr3, 99.9%), 1‐octadecene (ODE, technical grade 90%), methanol (CH3OH, 99.8%) were purchased from Alfa Aesar. Oleylamine (OAm, technical grade 90%) was purchased from Macklin. Toluene (C7H8, AR) was purchased from Beijing Chemical Works. Polystyrene (PS) was purchased from Acros. Polyvinylidene fluoride (PVDF) membrane with pore size of 0.2 µm was provided by Beyotime Biotechnology. All the chemicals were used as received without further purification.
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