Pan b magnetic beads

Manufactured by Miltenyi Biotec

Pan-B magnetic beads are a laboratory tool used for the isolation and enrichment of B cells from biological samples. These beads are coated with antibodies that specifically bind to B cell surface markers, allowing for the magnetic separation of B cells from other cell types.

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Lab products found in correlation

Rbc lysis, by Merck Group (2 mentions) Mouse tumor dissociation kit, by Miltenyi Biotec (1 mentions) Myeloid derived suppressor cell isolation kit, by Miltenyi Biotec (1 mentions) Rbc lysis, by Qiagen (1 mentions)

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Magnetic beads (547 citations)

3 protocols using pan b magnetic beads

Mouse Immune Cell Isolation

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Splenocytes were obtained from mice by mashing the spleens through cell strainers followed by RBC lysis (Sigma). Mouse B cells and CLL cells were purified from mouse spleens by negative selection using CD43 (Ly48) or Pan-B magnetic beads (Miltenyi Biotech), respectively, following the manufacturer’s instructions. MDSCs were purified from spleens, bone marrow or LLC tumors (digested with the mouse tumor dissociation kit purchased from Miltenyi Biotech) by positive selection using myeloid-derived suppressor cell isolation kit (Miltenyi Biotech).

Tang C.H., Chang S., Hashimoto A., Chen Y.J., Kang C.W., Mato A.R., Del Valle J.R., Gabrilovich D.I, & Hu C.C. (2018). Secretory IgM exacerbates tumor progression by inducing accumulations of MDSCs in mice. Cancer immunology research, 6(6), 696-710.

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Isolation of Mouse Splenocytes and B Cells

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Splenocytes were obtained from mice by mashing the spleens through cell strainers followed by RBC lysis (Sigma-Aldrich). Mouse B cells were purified from mouse spleens by negative selection using CD43 (Ly48) or pan-B magnetic beads (Miltenyi Biotec), according to the manufacturer’s instructions.

Tang C.H., Chang S., Paton A.W., Paton J.C., Gabrilovich D.I., Ploegh H.L., Del Valle J.R, & Hu C.C. (2018). Phosphorylation of IRE1 at S729 regulates RIDD in B cells and antibody production after immunization. The Journal of Cell Biology, 217(5), 1739-1755.

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Isolating Mouse Splenocytes and B Cells

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Splenocytes were obtained from mice by mashing the spleens through cell strainers followed by RBC lysis (Qiagen). Mouse B cells and Eμ-TCL1 CLL cells were purified from mouse spleens by negative selection using CD43 (Ly48) and Pan-B magnetic beads (Miltenyi Biotech), respectively, according to the manufacturer’s instructions.

Tang C.H., Zundell J.A., Ranatunga S., Lin C., Nefedova Y., Del Valle J.R, & Hu C.C. (2016). Agonist-mediated activation of STING induces apoptosis in malignant B cells. Cancer research, 76(8), 2137-2152.

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