Urine (50 mL) was collected in a sterile tube. All samples were cooled at 4 °C and processed within 30 min. Subsequently, urine samples were centrifuged (2500× g, 4 °C, 45 min) to remove cell debris. Urine samples, prior to freezing (−80 °C), were supplemented with the Calbiochem EDTA-free Protease Inhibitor Cocktail Set III (Merck Millipore, Burlington, MA, USA), at a dilution of 1/1000–1/2000, and sodium azide (NaN3, 0.02% v/v).
Calbiochem edta free protease inhibitor cocktail set 3
Manufactured by Merck Group
Sourced in United States
The Calbiochem EDTA-free Protease Inhibitor Cocktail Set III is a laboratory reagent designed to inhibit a broad spectrum of proteases. It is formulated without EDTA, making it suitable for use in applications where EDTA interference is a concern. The product is intended for research use only.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using calbiochem edta free protease inhibitor cocktail set 3
1
Urine Sample Preparation for Proteomic Analysis
2
Urine Proteome Profiling in Diabetic Nephropathy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
24 h urine was collected from five healthy controls and five participants of the Finnish Diabetic Nephropathy Study with type 1 diabetes and presenting constant macroalbuminuria. Calbiochem® EDTA‐free Protease Inhibitor Cocktail Set III (Merck Millipore, Burlington, MA) was added to voided urine at a dilution of 1/2000 before storage at −80°C. Donors information is given in Table 1 .
All experiments were performed in accordance with the Declaration of Helsinki and the study protocol was approved by the Ethical Committee of Helsinki and Uusimaa Hospital District. Participants signed a written informed consent prior to enrollment in the study.
All experiments were performed in accordance with the Declaration of Helsinki and the study protocol was approved by the Ethical Committee of Helsinki and Uusimaa Hospital District. Participants signed a written informed consent prior to enrollment in the study.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!