Hplc grade acetonitrile mecn

Manufactured by Avantor

Sourced in Germany, United States, Hungary

HPLC-grade acetonitrile (MeCN) is a high-purity solvent commonly used in high-performance liquid chromatography (HPLC) applications. It is a colorless, volatile liquid with a low viscosity and high polarity. HPLC-grade acetonitrile is designed to meet the stringent purity and quality requirements for HPLC analysis, ensuring consistent and reliable results.

Automatically generated - may contain errors

Lab products found in correlation

Milli q academic system, by Merck Group (1 mentions) Potassium phosphate monobasic kh2po4, by Merck Group (1 mentions) Methanol meoh, by Avantor (1 mentions) Rink amide mbha resin, by Iris Biotech (1 mentions) Fmoc gly novasyn tgt, by Merck Group (1 mentions) N n dimethylformamide (dmf), by Avantor (1 mentions) Piperidine, by Merck Group (1 mentions) Piperazine, by Merck Group (1 mentions) Diethyl ether, by Merck Group (1 mentions)

Close spelling variants of this product

Acetonitrile (857 citations) HPLC-grade acetonitrile (237 citations) HPLC grade (36 citations) Acetonitrile (MeCN) (7 citations)

4 protocols using hplc grade acetonitrile mecn

Optimizing Basil De-Greening Conditions

Check if the same lab product or an alternative is used in the 5 most similar protocols

HPLC-grade acetonitrile (MeCN)
was obtained from VWR International GmbH (Ismaning, Germany), and
ultrapure water (18 MΩ cm^–1) was obtained
from a Millipore S.A.S. Milli-Q Academic system (18.2 MΩ cm^–1, Molsheim, France). Potassium phosphate monobasic
(KH₂PO₄) and potassium phosphate dibasic (K₂HPO₄) were from Merck (Darmstadt, Germany). Potted
green basil plants were bought at local supermarkets and garden centers;
such basil plants were either analyzed fresh (green leaves) or kept
in house in partial shade over a time of 15 days and observed to nearly
uniformly de-green partially (see Figure 1A). Similar de-greening was also observed
with plants kept outside at moderate summer temperatures in a semi-shaded
area.

Moser S., Erhart T., Neuhauser S, & Kräutler B. (2020). Phyllobilins from Senescence-Associated Chlorophyll Breakdown in the Leaves of Basil (Ocimum basilicum) Show Increased Abundance upon Herbivore Attack. Journal of Agricultural and Food Chemistry, 68(27), 7132-7142.

+ Open protocol

+ Expand

HPLC Solvent Preparation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

HPLC grade acetonitrile (MeCN) and methanol (MeOH) were from VWR (West Chester, USA), ultra-pure water (18 MΩ.cm⁻¹) from a Millipore apparatus, potassium phosphate monobasic (KH₂PO₄), puriss. p.a., potassium phosphate dibasic (K₂HPO₄), puriss. p.a., and ammonium acetate, puriss. p.a. were from Fluka (Buchs, CH). 1 g SepPak C18 cartridges were from Waters Associates (Milford, CT, USA). Media and chemicals were obtained from Sigma-Aldrich (Vienna, Austria) if not stated otherwise.
pH-measurements: WTW Sentix 21 electrode connected to a WTW pH 535 digital pH meter.

Karg C.A., Parráková L., Fuchs D., Schennach H., Kräutler B., Moser S, & Gostner J.M. (2022). A Chlorophyll-Derived Phylloxanthobilin Is a Potent Antioxidant That Modulates Immunometabolism in Human PBMC. Antioxidants, 11(10), 2056.

+ Open protocol

+ Expand

Peptide Synthesis Using MBHA Resin

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rink-amide MBHA resin and all amino acid derivatives were purchased from Iris Biotech GmBH (Marktredwitz, Germany), except for N-Fmoc-L-Statine, which was obtained from Fluorochem Ltd. (Hadifield, UK). Aminooxyacetic acid, scavengers, coupling agents (1-hydroxybenzotriazole hydrate (HOBt), N,N′-diisopropylcarbodiimide (DIC)), and cleavage reagents (triisopropylsilane (TIS), piperidine, 1,8-diazabicyclo(5.4.0)undec-7-ene (DBU), 1,2-ethandithiol (EDT), thioanisole), diisopropylethylamine (DIPEA) and acetic anhydride (Ac₂O) were purchased from Sigma Aldrich Kft. (Budapest, Hungary). Daunorubicin hydrochloride was provided by IVAX (Budapest, Hungary). N,N-Dimethylformamide (DMF), dichloromethane (DCM), diethyl ether (Et₂O), trifluoroacetic acid (TFA) and HPLC grade acetonitrile (MeCN) were purchased from VWR Chemicals (Debrecen, Hungary). All reagents and solvents were of analytical grade or highest available purity.

Gomena J., Vári B., Oláh-Szabó R., Biri-Kovács B., Bősze S., Borbély A., Soós Á., Ranđelović I., Tóvári J, & Mező G. (2023). Targeting the Gastrin-Releasing Peptide Receptor (GRP-R) in Cancer Therapy: Development of Bombesin-Based Peptide–Drug Conjugates. International Journal of Molecular Sciences, 24(4), 3400.

+ Open protocol

+ Expand

Peptide Synthesis and Purification

Check if the same lab product or an alternative is used in the 5 most similar protocols

All Fmoc-protected amino acids were purchased
from ChemPep Inc. or Iris Biotech GmbH. The following resins were
used: Fmoc-Tentgel R Ram Rink-type resin (0.18 mmol/g, Peptide International,
Fmoc-Gly-Novasyn TGT (0.2 mmol/g), Merck KGaA and Fmoc-Gly(Dmb)-Tentagel
(0.18 mmol/g), Rapp Polymere GmbH. Di-Fmoc-3,4-diaminobenzoic acid
was from AnaSpec. 2-(1H-Benzotriazole-1-yl)-1,1,3,3-tetramethyluronium
hexafluorophosphate) (HBTU) was purchased from PepChem. N,N-Dimethylformamide (DMF) was from Honeywell Burdick
& Jackson. HPLC-grade acetonitrile (MeCN) was from VWR Chemicals.
Diethyl ether was purchased from Merck KGaA. Diisopropylethylamine
(DIPEA), triisopropylsilane (TIPS), piperidine, piperazine, ethyl(hydroxyamino)
cyanoacetate (Oxyma pure), 1-hydroxybenzotriazole hydrate (HOBT),
trifluoroacetic acid (TFA), tris(2-carboxyethyl)phosphine hydrochloride
(TCEP·HCl), N-ethylmaleimide (NEM), and iodoacetamide
(IAM) were from Sigma-Aldrich. PD-10 desalting columns (Sephadex G-25
resin) for size exclusion chromatography were from GE Healthcare Bio-Sciences.

Muhammad T., Houssen W.E., Thomas L., Alexandru-Crivac C.N., Gunasekera S., Jaspars M, & Göransson U. (2023). Exploring the Limits of Cyanobactin Macrocyclase PatGmac: Cyclization of PawS-Derived Peptide Sunflower Trypsin Inhibitor-1 and Cyclotide Kalata B1. Journal of Natural Products, 86(3), 566-573.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!