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Multiclamp 700a and 700b

Manufactured by Molecular Devices
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The Multiclamp 700A and 700B are versatile, high-performance electrophysiology amplifiers designed for intracellular recordings from a wide range of cell types. These amplifiers provide precise, low-noise signal acquisition and amplification, enabling researchers to study the electrical properties of cells with high fidelity.

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Lab products found in correlation

Axoclamp 900a, by Molecular Devices (2 mentions) Pclamp 9 0 and 10, by Molecular Devices (2 mentions) P 1000, by Sutter Instruments (2 mentions) Digidata 1322, by Molecular Devices (2 mentions)

Spelling variants of this product

Multiclamp 700B (283 citations) Multiclamp 700A (54 citations)

2 protocols using multiclamp 700a and 700b

1

Patch-Clamp Recording of Isolated Cardiomyocytes

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Isolated LV myocytes were placed in a bath on the stage of an IX51, IX53, and IX71 microscopes for patch-clamp measurements11 (link)12 (link)32 (link)55 (link)56 (link)66 (link). Experiments were conducted at room temperature. Data were acquired by means of the whole-cell patch-clamp technique in voltage- and current-clamp modes using Multiclamp 700A and 700B, and Axoclamp 900A amplifiers (Molecular Devices). Electrical signals were digitized using 250 kHz 16-bit resolution A/D converters (Digidata 1322, 1440A, and 1550, Molecular Devices) and recorded using pCLAMP 9.0 and 10 software (Molecular Devices) with low-pass filtering at 2 kHz. Membrane capacitance (Cm) was measured in voltage-clamp mode using a 5 mV voltage step and pCLAMP software algorithm. For each cell, current density was obtained by dividing transmembrane currents recorded in voltage-clamp by the measured Cm, providing a normalization of ionic currents with respect to cell size. Current density was expressed in pA pF−1 (refs 11 (link), 12 (link), 32 (link), 55 (link), 56 (link)). Pipettes were pulled by means of a vertical (PB-7, Narishige), or horizontal (P-1000, Sutter Instrument) glass microelectrode pullers; when filled with intracellular solution pipettes had a resistance of 1–2 MΩ.
Signore S., Sorrentino A., Borghetti G., Cannata A., Meo M., Zhou Y., Kannappan R., Pasqualini F., O'Malley H., Sundman M., Tsigkas N., Zhang E., Arranto C., Mangiaracina C., Isobe K., Sena B.F., Kim J., Goichberg P., Nahrendorf M., Isom L.L., Leri A., Anversa P, & Rota M. (2015). Late Na+ current and protracted electrical recovery are critical determinants of the aging myopathy. Nature Communications, 6, 8803.
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2

Patch-Clamp Measurements of Isolated LV Myocytes

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Isolated LV myocytes were placed in a bath on the stage of an IX51, IX53, and IX71 (Olympus) microscopes for patch-clamp measurements11 (link),12 (link),32 (link),55 (link),56 (link),66 (link). Experiments were conducted at room temperature. Data were acquired by means of the whole-cell patch-clamp technique in voltage- and current-clamp modes using Multiclamp 700A and 700B, and Axoclamp 900A amplifiers (Molecular Devices). Electrical signals were digitized using 250 kHz 16-bit resolution A/D converters (Digidata 1322, 1440A, and 1550, Molecular Devices) and recorded using pCLAMP 9.0 and 10 software (Molecular Devices) with low-pass filtering at 2 kHz. Membrane capacitance (Cm) was measured in voltage-clamp mode using a 5 mV voltage step and pCLAMP software algorithm. For each cell, current density was obtained by dividing transmembrane currents recorded in voltage-clamp by the measured Cm, providing a normalization of ionic currents with respect to cell size. Current density was expressed in pA/pF11 (link),12 (link),32 (link),55 (link),56 (link). Pipettes were pulled by means of a vertical (PB-7, Narishige), or horizontal (P-1000, Sutter Instrument) glass microelectrode pullers; when filled with intracellular solution pipettes had a resistance of 1–2 MΩ.
Signore S., Sorrentino A., Borghetti G., Cannata A., Meo M., Zhou Y., Kannappan R., Pasqualini F., O'Malley H., Sundman M., Tsigkas N., Zhang E., Arranto C., Mangiaracina C., Isobe K., Sena B.F., Kim J., Goichberg P., Nahrendorf M., Isom L.L., Leri A., Anversa P, & Rota M. (2015). Late Na+ current and protracted electrical recovery are critical determinants of the aging myopathy. Nature Communications, 6, 8803.
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