Abi prism 310 genetic analyser
The ABI Prism 310 Genetic Analyzer is a capillary electrophoresis-based system designed for DNA sequencing and fragment analysis. It utilizes laser-induced fluorescence detection and provides accurate and reliable data for various genetic applications.
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24 protocols using abi prism 310 genetic analyser
Microsatellite Instability Analysis Protocol
Molecular Profiling of Oligodendroglioma
Insect Species Identification via PCR
Genetic Analysis of Nephrotic Syndrome Genes
DNA Methylation Analysis by MSAM
Sequencing and Phylogenetic Analysis of Viral Genomes
Sequences were aligned using GramAlign v3.0 [33 (link)]. A Maximum clade credibility (MCC) tree with dated tips and internal nodes was inferred using a MCMC Bayesian approach under the GTR model with gamma-distributed rate variation (Γ) and a proportion of invariable sites (I) using a relaxed (uncorrelated lognormal) molecular clock [34 (link)] in BEAST version 1.8.4 [35 (link)]. Four independent MCMC runs of four chains each were run for 10,000,000 states. The First 1,000,000 were used as burning and the MCC was establish from the remaining states. A median joining network [36 (link)] of the sequences was constructed and edited using a 2,190-character set in SPLITSTREE v4.12.3 [37 (link)]
Molecular Profiling of Myeloproliferative Neoplasms
Myeloproliferative leukaemia (MPL) proto‐oncogene, thrombopoietin receptor (MPL) mutations, in particular W515L, W515K, W515A, S505N, and G509C, were tested by direct sequencing of exon 10. The primers used were as follows: MPL10F 5' TAGCCTGGATCTCCTTGGTG 3′; MPL10R 5' CCTGTTTACAGGCCTTCGGC 3′.
Mutations in exon 9 of the calreticulin (CALR) gene were also assessed using a bidirectional sequencing approach as previously described.
FBN1 Gene Mutation Screening
Step 1: In the first phase, we screened for mutations of the FBN1 gene with the use of next-generation sequencing (NGS) technique as previously described [31 (link)]. We applied a Roche GS Junior platform.
Step 2: Homopolymer regions were investigated with Sanger sequencing with the use of ABI Prism 310 Genetic Analyser (Applied Biosystems) and all the detected (likely) pathogenic mutations were confirmed by this technique.
Genotyping HOXB13 Germline Mutation
Mitochondrial 16S rDNA Amplification and Sequencing
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