Cd8 c8 144b

Manufactured by Thermo Fisher Scientific

The CD8 C8/144B is a laboratory instrument designed for the detection and enumeration of CD8+ T cells. It utilizes flow cytometry technology to analyze cell samples and provide quantitative data on CD8-positive lymphocytes.

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Lab products found in correlation

Bond max, by Leica (1 mentions) Pd l1 clone e1l3n, by Cell Signaling Technology (1 mentions) Cd3 a0452, by Agilent Technologies (1 mentions) Mrq 42, by Cell Marque (1 mentions)

Close spelling variants of this product

CD8 clone C8/144B (4 citations) Anti-CD8 (clone C8/144B, (2 citations)

2 protocols using cd8 c8 144b

Automated Multiplex IHC Analysis

Cited 1 time

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Immunohistochemistry for TUNEL and Ki-67 was performed as previously described (19 (link)). For the PROSPECT samples 4μm- thick tissue sections were stained using an automated staining system (Leica Bond Max, Leica Microsystems, Vista, CA, USA), according to standard protocols. The38 Aperio Image Analysis Toolbox (Aperio, Leica Microsystems) was used for digital analysis of images obtained from scanned slides. PDL1 clone E1L3N from Cell Signaling Technologies, CD3 A0452 from Dako and CD8 C8/144B from Thermo Scientific were used.

Koyama S., Akbay E.A., Li Y.Y., Aref A.R., Skoulidis F., Herter-Sprie G.S., Buczkowski K.A., Liu Y., Awad M.M., Denning W.L., Diao L., Wang J., Parra-Cuentas E.R., Wistuba I.I., Soucheray M., Thai T.C., Asahina H., Kitajima S., Altabef A., Cavanaugh J.D., Rhee K., Gao P., Zhang H., Fecci P.E., Shimamura T., Hellmann M.D., Heymach J.V., Hodi F.S., Freeman G.J., Barbie D.A., Dranoff G., Hammerman P.S, & Wong K.K. (2016). STK11/LKB1 deficiency promotes neutrophil recruitment and proinflammatory cytokine production to suppress T cell activity in the lung tumor microenvironment. Cancer research, 76(5), 999-1008.

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Immunohistochemical Assessment of Tumor Microenvironment

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Immunohistochemical stains were performed on 4 µm TMA tissue sections on a Ventana Benchmark Ultra autostainer (Ventana) for the following proteins: CD3 (F7.2.38, monoclonal mouse, ThermoFisher-Scientific, dilution 1:50), CD8 (C8/144B, mouse monoclonal, ThermoFisher-Scientific, dilution 1:50), CD68 (PG-M1, mouse monoclonal, ThermoFisher-Scientific, dilution 1:60), PD-1 (NAT105, mouse monoclonal; Ventana) and CD56 (MRQ-42, monoclonal mouse, CELL MARQUE, dilution 1:50) and PD-L1 (SP263 assay, Ventana).
PD-L1⁺ ICs and tumor cells (TCs) were scored by two pathologists (AH, ME) according to the distributor’s PD-L1 scoring algorithm.19 20 (link) Positivity of TC was scored as a percentage of positive TC in relation to TC area. Number of ICs was scored as the percentage ratio of the area occupied by positive ICs per tumor area.19 (link)

Eckstein M., Strissel P., Strick R., Weyerer V., Wirtz R., Pfannstiel C., Wullweber A., Lange F., Erben P., Stoehr R., Bertz S., Geppert C.I., Fuhrich N., Taubert H., Wach S., Breyer J., Otto W., Burger M., Bolenz C., Keck B., Wullich B., Hartmann A, & Sikic D. (2020). Cytotoxic T-cell-related gene expression signature predicts improved survival in muscle-invasive urothelial bladder cancer patients after radical cystectomy and adjuvant chemotherapy. Journal for Immunotherapy of Cancer, 8(1), e000162.

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