Stressed and normal plant samples (rosette leaves) were harvested and finely ground in liquid nitrogen with a mortar and pestle. Each sample was then weighed (50 mg) and transferred into 2 mL Eppendorf tubes. Next, working solution of standards (20 μL) was added to each sample. The samples were then extracted using 9.8 mL of MA. After vortexing, samples were centrifuged at 13,000 ×g for 5 min at 4°C. The supernatant was then collected (1 mL) and filtered through a 0.45 μM nylon syringe filter (Whatman, Korea), after which each sample (10 μL) was injected into a UFLC-MS/MS system for hormonal analysis.
Nylon syringe filter
Manufactured by Cytiva
Sourced in United Kingdom
Nylon syringe filters are disposable laboratory filters designed to remove particulates from small-volume liquid samples. They feature a nylon membrane with a pore size typically ranging from 0.2 to 0.45 microns, which allows the sample to pass through while retaining unwanted particles.
Automatically generated - may contain errors
Lab products found in correlation
Ultrapure water, by Merck Group (3 mentions)
Lc 20avp, by Shimadzu (3 mentions)
Rid 20a vp, by Shimadzu (2 mentions)
Class vp chromatography manager software, by Shimadzu (2 mentions)
5975c series, by Agilent Technologies (1 mentions)
7683b series, by Agilent Technologies (1 mentions)
Methanol, by Merck Group (1 mentions)
Db 5ms column, by Agilent Technologies (1 mentions)
Gc 7890a, by Agilent Technologies (1 mentions)
Combi pal, by CTC Analytics (1 mentions)
Xylose, by Merck Group (1 mentions)
Prominence lc 20a, by Shimadzu (1 mentions)
Fructose, by Merck Group (1 mentions)
Nanosight lm10, by Malvern Panalytical (1 mentions)
Agilent 1260 infinity 2 hplc system, by Agilent Technologies (1 mentions)
Spd 20avp, by Shimadzu (1 mentions)
14 protocols using nylon syringe filter
1
Extraction and Quantification of Plant Hormones
2
Chemical Analysis of Organic Solvents in PR Products
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Organic solvents contained in PR products were analyzed after dilution with carbon disulfide (CS2; Kanto, Japan) and methanol (99.8%; Sigma Aldrich, USA). Qualitative analysis was first performed to identify organic solvents in 51 PR products, and further analysis was conducted for quantitation of the identified 20 chemicals. Diluted samples were sonicated for 30 min at room temperature, and viscous chemical samples were filtered through a nylon syringe filter (13 mm, 0.2 μm, Whatman, USA). Qualitative analysis was conducted by gas chromatography (GC, 7890A; Agilent Technology, USA)–mass spectrometry (MS, 5975C Series; Agilent Technology, USA) and auto sampler (Combi PAL, CTC analytics, Switzerland) in scan mode. A DB-5MS column (122-5532; Agilent Technology, USA) was used for analysis. Each mass spectrum was matched up with a GC-MS library (W10N11), and the chemical matching rate selected was higher than 80%.
Then, quantitative analysis was conducted by GC (6890N; Agilent Technology) with a flame ionization detector (FID) and auto sampler (7683B Series; Agilent Technology). The chemical used for quantitative analysis was selected from chemicals detected from the qualitative analysis listed in the MSDS, or if not listed in the MSDS, a chemical known to be toxic was selected. An EN-5 column (053139; SGE Analytical Science, Australia) was used for the analysis.
Then, quantitative analysis was conducted by GC (6890N; Agilent Technology) with a flame ionization detector (FID) and auto sampler (7683B Series; Agilent Technology). The chemical used for quantitative analysis was selected from chemicals detected from the qualitative analysis listed in the MSDS, or if not listed in the MSDS, a chemical known to be toxic was selected. An EN-5 column (053139; SGE Analytical Science, Australia) was used for the analysis.
3
Melatonin Stability under Different pH Conditions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Melatonin solutions were prepared as a previous study (Daya et al., 2001 (link)), with slight modifications. One milliliter of standard stock solution of melatonin (0.1 μg/ml) was added in 100 ml phosphate buffer solutions (final concentration: 0.001 μg/ml) at pH 1, 4, 7, 10 and 13 and adjusted by 0.1 M HCl and 0.1 M NaOH. Ten milliliter aliquots of melatonin solution were placed in 20 ml amber glass bottles with screw cap to minimize the amount of light and stored at room temperature for 28 days. Each solution was randomly taken out every 7 days (7, 14, 21, 28 days) at the same time and assayed for melatonin concentration. An aliquot of each sample was taken up in a syringe and filtered through a 0.22 μm nylon syringe filter (Whatman plc) and the filtered solution was injected into a liquid chromatography-tandem mass spectrometry system to determine melatonin concentration.
4
Extraction and Purification of Breast Meat Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Extraction and purification were performed using the method described in [14 (link)] and [16 (link)] with slight modifications. Minced breast meat samples (2.5 g) were homogenized in 10 mL deionized distilled water using a WiseTis HG-15 D homogenizer (Wisd, Witeg, Wertheim, Germany) at 25,000 rpm for 1 min in a 15 mL polyethylene tube and centrifuged at 9989× g for 5 min at 4 °C. The supernatant was collected and incubated at 80 °C for 10 min in a temperature-controlled water bath, followed by centrifugation at 9989× g for 5 min at 4 °C. Then, 1 mL supernatant was filtered through a nylon syringe filter (0.45 µm, Whatman, Maidstone, UK) into a vial tube of 1.5 mL and stored at −20 °C for HPLC analysis.
5
OTA Recovery from Spiked Wine
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Wine was spiked with different concentrations of OTA (0.05, 0.5, 1, 2 and 3 µg L -1 ), diluted 1:4 with 0.1 M Tris buffer pH 8.0 (final pH approx. 7.2) and filtered through a 0.45 µm nylon syringe filter (Whatman). SPE columns with 1.5 mL of BSA-agarose were equilibrated with 5 mL of 0.01 M Tris buffer pH 7.0, loaded with 20 mL of the wine/ buffer mixture, washed with 5 mL of 0.01 M Tris buffer pH 7.0 and eluted with 4 mL of methanol containing 1% (v/v) of acetic acid. The eluate was then evaporated at 40 °C with a slight nitrogen flow and resuspended in 0.5 mL of HPLC mobile phase. The samples were preserved at 4 °C until OTA quantification by HPLC-FL. Two independent assays for each OTA concentration were performed.
6
Quantifying Strawberry Sugar Profiles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Changes in glucose, fructose, sucrose and total sugar content in the juice obtained from the harvested strawberries were determined according to the method developed by Akšić et al. [31 (link)] Lyophilized strawberry fruit samples were powdered using a mortar and pestle. Then, they were weighed to 0.1 g. A total of 5 mL of ultrapure water (Millipore Corp., Bedford, MA, USA) was added to each sample. The reaction mixture was placed in an ultrasonic bath and sonicated at 80 °C for 15 min, and then centrifuged at 5500 rpm for 15 min and filtered prior to HPLC analysis. (Whatman nylon syringe filters, 0.45 µm, 13 mm, diameter). The high-performance liquid chromatographic apparatus (Shimadzu LC 20A vp, Kyoto, Japan) consisted of an in-line degasser, pump and controller coupled to a refractive index detector (Shimadzu RID 20A vp) equipped with an automatic injector (20 µL injection volume) interfaced to a PC running Class VP chromatography manager software (Shimadzu, Japan).
Separations were performed on a 300 mm × 7.8 mm i.d., 5 µm, reverse-phase Ultrasphere Coregel-87 C analytical column (Transgenomic, Omaha, NE, USA) operating at 70 °C with a flow rate of 0.6 mL min−1. Elution was isocratic ultrapure water. Individual sugars were calculated based on their standards and expressed in mg/100 g of dry weight (DW).
Separations were performed on a 300 mm × 7.8 mm i.d., 5 µm, reverse-phase Ultrasphere Coregel-87 C analytical column (Transgenomic, Omaha, NE, USA) operating at 70 °C with a flow rate of 0.6 mL min−1. Elution was isocratic ultrapure water. Individual sugars were calculated based on their standards and expressed in mg/100 g of dry weight (DW).
7
Extraction of PPO and POD Enzymes from Dates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A crude extract of the PPO and POD enzymes was obtained using the method described elsewhere [12 (link)]. In this method, four grams of date sample was homogenized in 16 ml of 0.5 M of potassium phosphate buffer (K3PO4) (pH 6.8) and 0.4 g of polyvinylpyrrolidone (PVP) in an ice bath for three minutes using a homogenizer (IKA T-18 Ultra Turrax Digital Homogenizer, Germany). After one minute of homogenization, the sample was let to rest for 20 s to avoid overheating. The homogenate was then centrifuged at 14,000 rpm for 20 min at 4°C. The supernatant was collected and filtered through 0.2 μm nylon syringe filters (Whatman, UK). The filtered supernatant was used immediately for PPO and POD activity assessment.
8
Quantification of Sugars in Pitaya Fruit
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Glucose, fructose, xylose and total sugar contents in homogenized pitaya samples were determined using the HPLC technique according to the method developed by Cristosto [48 ]. Glucose, fructose and xylose (Sigma-Aldrich, St. Louis, MO, USA) are used as external standards (15–2500 ppm) for sugar analysis. Before analysis, frozen fruit samples were thawed at 25 °C. One g of homogenized fruit sample was added to 4 mL of ultrapure water (Millipore Corp., Bedford, MA, USA). The reaction mixture was placed in an ultrasonic bath and sonicated at for 15 min and then centrifuged at 5500 rpm for 15 min and it was filtered before HPLC analysis (Whatman nylon syringe filters, 0.45 µm, 13 mm, diameter). Triplicate analysis was completed and HPLC (Shimadzu, Prominence LC-20A) RID (Refractive Index) detector and Coregel-87C (7.8 × 300 mm) HPLC column were used. Separations were performed at 70 °C at a flow rate of 0.6 mL/min. Elution was isocratic ultrapure water. The individual sugars were calculated according to their standards and expressed as percent fresh weight (FW). Calibration curves of all references were created and content was determined according to for the calibration curves for Glucose y = 266.56x + 0; fructose y = 253.36x + 0; xylose y = 256.48x + 0 (Those r2 = 0.999)
9
Nanoparticle Characterization Using NTA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sample particle concentrations and size distributions were measured using a NanoSight LM10 (Malvern Panalytical) equipped with a 405 nm blue laser and sCMOS camera. 1000-fold dilutions of EV isolates prepared as described above where thawed. Ultrapure water used to prepare dilutions was filtered through pre-wet 0.1 μm Nylon syringe filters (Whatman) immediately prior to measurement. Filtered ultrapure water was also used to flush the NTA chamber and tubing before sample addition to ensure contamination was minimized during measurement. 1 mL of each diluted sample was loaded into a syringe and placed on an automatic syringe pump for injection. Data was recorded as three 30 s videos containing a minimum of 200 particle tracks per video, recorded at camera level 12. NTA 3.1 software was used to analyze the data and track the Brownian motion of the individual particles recorded. Subsequently the software calculated hydrodynamic diameters (nm) of the tracked particles using the Einstein-Stokes relation, and the count-based concentrations (particles per mL) are simultaneously obtained as the number of particles and volume of the sample chamber are known.
10
HPLC Analysis of CBD Release from Silica Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The quantity of CBD released from slurry and dried CBD-Silica samples was determined with an Agilent 1260 Infinity II HPLC system (Agilent Technologies Inc., Santa Clara, CA, USA). Acetonitrile/water with 0.1% trifluoroacetic acid was used as mobile phase at a ratio of 75/25 (v/v) in an isocratic mode. A reverse-phase C18 column (5 μm, 250 × 4.6 mm; Grace Davison Discovery Sciences, Deerfield, IL, USA) was used, and the UV absorption was detected at 210 nm wavelength. The injection volume and flow rate were set at 20 μL and 0.8 mL/min, respectively. CBD calibration standards were prepared in ethanol (100%) at concentrations of 0.01, 0.05, 0.1, 0.5, 1, 5 and 10 μg/mL. Before the HPLC session, all samples were filtered through 0.2 μm nylon syringe filters (Whatman, Maidstone, UK).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!