Rabbit ig control

Manufactured by Abcam

The Rabbit Ig control is a laboratory reagent that serves as a reference standard for immunoglobulin (Ig) detection and quantification experiments. It provides a consistent, reliable, and well-characterized sample to validate the performance of immunoassays targeting rabbit immunoglobulins.

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Lab products found in correlation

Spin column, by Qiagen (1 mentions) C jun antibody, by Abcam (1 mentions) Sybr green real time pcr, by Thermo Fisher Scientific (1 mentions)

2 protocols using rabbit ig control

ChIP Analysis of c-Jun Binding

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ChIP analysis was performed as previously described 11 (link). Chromatin solutions were precipitated overnight at 4 °C using c-Jun antibody or rabbit Ig control (Abcam, Cambridge, MA). The input DNA and the immunoprecipitated DNA were extracted using Qiagen spin columns and were analyzed by PCR or real-time PCR assays using Ei and E3′ specific primers (Table S1).

Ding X., Wang X., Zhu X., Zhang J., Zhu Y., Shao X, & Zhou X. (2020). JNK/AP1 Pathway Regulates MYC Expression and BCR Signaling through Ig Enhancers in Burkitt Lymphoma Cells. Journal of Cancer, 11(3), 610-618.

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EGR2 Binding to Rorc and Il17a Promoters

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To test for EGR2 binding to the Rorc and Il17a promoters, we performed chromatin immunoprecipitation (ChIP)-qPCR in 48h activated T_H17(β,6) cells. Naïve 2D2 CD4⁺ T cells were activated with plate-bound anti-CD3 antibody (20 μg/ml) and anti-CD28 antibody (4 μg/ml) for 48h in the presence of T_H17(β,6) cell-polarizing conditions. T_H17(β,6) cells were fixed in 1% formaldehyde in PBS (final concentration) for 10 minutes at RT with gentle shaking. ChIP assays were performed using SimpleChIP Enzymatic Chromatin IP Kit (Cat. No. 9005; Cell Signaling) using rabbit anti-mouse EGR2 antibody (Cat. No. 43020; Abcam) or rabbit Ig control (Cat. No. 2729S; Cell Signaling), following manufacturer’s instructions. Primers specific for test regions containing EGR binding sites and for control regions were used to amplify the ChIP-enriched and input DNA by SYBR Green real-time PCR (Applied Biosystems). Data are presented as percentage of input DNA. Primers are listed (5’ to 3’ sequence, forward and reverse strand) in Supplementary Table 5.

Gao Y., Wang Y., Chauss D., Villarino A.V., Link V.M., Nagashima H., Spinner C.A., Koparde V.N., Bouladoux N., Abers M.S., Break T.J., Chopp L.B., Park J.H., Zhu J., Wiest D.L., Leonard W.J., Lionakis M.S., O’Shea J.J., Afzali B., Belkaid Y, & Lazarevic V. (2023). The transcription factor EGR2 controls homing and pathogenicity of TH17 cells in the central nervous system. Nature immunology, 24(8), 1331-1344.

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