Cd4 alexa 647

Manufactured by BioLegend

Sourced in United States

CD4-Alexa-647 is a fluorescently-labeled antibody that binds to the CD4 cell surface receptor. It is used for the identification and enumeration of CD4+ T cells in flow cytometry applications.

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Close spelling variants of this product

Anti-CD4 Alexa Fluor 647 (GK1.5; Alexa Fluor® 647 anti-mouse CD4 CD4 Alexa Fluor 647 Alexa 647

4 protocols using cd4 alexa 647

Multiparametric Analysis of T Cell Responses

Cited 2 times

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Intracellular IL-2 and IFN-γ cytokine production, DNA damage marker γH2AX expression, PKH26-label CD4 T cell proliferation, telomere length measured by florescence in-situ hybridization (Flow-FISH), and cell apoptosis assay for Av/7AAD expression were analyzed by flow cytometry, as described previously [26 (link), 27 (link)]. The following reagents were used in the assays: PE-labelled Av, IL-2, IFN-γ, γH2AX (BD Biosciences, San Jose, CA), CD4-Alexa-647 (BioLegend, San Diego, CA), telomere probe TelC (CCCTAACCCTAACCCTAA)-FITC (0.25 μg probe/mL, PNA Bio, Newbury Park, CA).

Ji Y., Dang X., Nguyen L.N., Nguyen L.N., Zhao J., Cao D., Khanal S., Schank M., Wu X.Y., Morrison Z.D., Zou Y., El Gazzar M., Ning S., Wang L., Moorman J.P, & Yao Z.Q. (2019). Topological DNA damage, telomere attrition and T cell senescence during chronic viral infections. Immunity & Ageing : I & A, 16, 12.

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Multiparametric Flow Cytometry Analysis

Cited 1 time

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Intracellular IL-2, IFN-γ cytokine production, DNA damage marker γH2AX expression, CSFE-label CD4 T cell proliferation, telomere length measured by Flow-FISH, and cell apoptosis assay for Av/7AAD expression were analyzed by flow cytometry, as described previously^{5 –7 (link)}. The following reagents were used in the assays: PE-labelled Av, 7-AAD, IL-2, IFN-γ, γH2AX (BD), CD4-Alexa-647 (Bio legend), telomere probe Tel-C (TAACCC)-FITC (0.25 µg probe/mL, PNA Bio, Newbury Park, CA).

Dang X., Ogbu S.C., Zhao J., Nguyen L.N., Cao D., Nguyen L.N., Khanal S., Schank M., Thakuri B.K., Wu X.Y., Morrison Z.D., Zhang J., Li Z., El Gazzar M., Ning S., Wang L., Wang Z., Moorman J.P, & Yao Z.Q. (2020). Inhibition of topoisomerase IIA (Top2α) induces telomeric DNA damage and T cell dysfunction during chronic viral infection. Cell Death & Disease, 11(3), 196.

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Comprehensive Multicolor Flow Cytometry Panel

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Biolegend: CD19-PE (clone HIB19), CD4-Alexa647 (clone OKT4), CD4-BV510 (clone peripheral), CD3-BV605 (clone OKT3), CD69-PE (clone FN50), CD69-FITC (clone FN50), CD107a-BV421 (clone H4A3), PD-1-PECy7 (clone EN12.2H7), IFNγ-BV785 (clone 4S.B3), TNFα-Alexa647 (clone Mab-11), CD40L-PEDazzle594 (clone 24–31),
Becton Dickinson: CD3-FITC (clone UCHT-1), CD8-PerCPCy5.5 (clone RPA-T8), CD25-APC-H7 (clone M-A251), Granzyme B-A700 (clone GB11), Fc block (Clone: 3070), Fixable Viability Stain 520 (BD Horizon), Live/Dead UV450/50 (Invitrogen), Cell Trace Violet (Invitrogen), His-PE (Miltenyi, clone GG11-8F3.5.1), anti-6X His tag antibody [HIS.H8] (AbCAM, Cat. No. ab18184), IRDye 800CW Goat anti-mouse IgG secondary antibody (Li-COR Biosciences, Cat. No. 926–32210).

Gardell J.L., Matsumoto L.R., Chinn H., DeGolier K.R., Kreuser S.A., Prieskorn B., Balcaitis S., Davis A., Ellenbogen R.G, & Crane C.A. (2020). Human macrophages engineered to secrete a bispecific T cell engager support antigen-dependent T cell responses to glioblastoma. Journal for Immunotherapy of Cancer, 8(2), e001202.

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Multicolor Flow Cytometry and Immunohistochemistry

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For FACS analysis, the following fluorochrome-labeled antibodies specific for mouse molecules were used: CD4-FITC, CD4-Alexa647, CD19-Pacific blue (BioLegend, Inc., San Diego, CA, USA), and GL7-FITC and FAS-PE (Becton Dickinson Ltd., Franklin Lakes, NJ, USA). For immunohistochemistry, anti-mouse-IgM-Alexa488, anti-rat-IgG-Alexa488, and anti-mouse-IgG-Alexa555 (Invitrogen Life Technologies, Carlsbad, CA, USA), anti-mouse-IgD-biotin (BioLegend), GL7-Alexa488 (eBiosciences Inc., San Diego, CA, USA), and anti-mouse IgM F(ab’)₂ Alexa647 (Jackson ImmunoResearch laboratories Inc., West grove, PA, USA) were used.

Kanari Y., Sugahara–Tobinai A., Takahashi H., Inui M., Nakamura A., Hirose S, & Takai T. (2014). Dichotomy in FcγRIIB deficiency and autoimmune-prone SLAM haplotype clarifies the roles of the Fc receptor in development of autoantibodies and glomerulonephritis. BMC Immunology, 15, 47.

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