The GC-MS analysis was carried out with an Agilent 5975 GC-MSD system. Innowax FSC column (60 m × 0.25 mm, 0.25 mm film thickness) was used with helium as carrier gas (0.8 mL/min). GC oven temperature was kept at 60 °C for 10 min and programmed to 220 °C at a rate of 4 °C/min, and kept constant at 220 °C for 10 min and then programmed to 240 °C at a rate of 1 °C/min. Split ratio was adjusted at 40:1. The injector temperature was set at 250 °C. Mass spectra were recorded at 70 eV. Mass range was from m/z 35 to 450.
5975 gc msd system
Manufactured by Agilent Technologies
The 5975 GC-MSD system is a gas chromatograph-mass spectrometer (GC-MS) from Agilent Technologies. It is designed for the separation, identification, and quantification of chemical compounds in complex samples. The system combines a gas chromatograph for sample separation with a mass spectrometer for sensitive and selective detection of the separated compounds.
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17 protocols using 5975 gc msd system
1
GC-MS Analysis of Chemical Compounds
2
GC-MS Analysis of Oregano Volatile Oil
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Analyses by GC–MS were carried out by an Agilent 5975 GC-MSD system. HP 5MS equipped with a DB-1MS column (30×250 µm, 0.25 μm) were used with gas of helium as a mobile phase (40 mL/min). The temperature program was 50°C for 5 minutes, 5°C/min to 240°C, then 10°C/min to 290°C. The split ratio was 1:50 in this procedure. The injector temperature was modified to 290°C and mass spectra were recorded at 70 eV. The mass range was set up from 35 to 450 m/z. For the injection (split injector), 5 μL of essential oil was diluted in 500 μL of hexane, and 5 μL of this diluted solution was injected. Identification and quantity of O. vulgare’s volatile oil constituents were made on the basis of their retention times, the peak area of spectrograms and their mass spectra, which were confirmed by comparison with data from the Wiley mass spectral library.22
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Analyses by GC–MS were carried out by an Agilent 5975 GC-MSD system. HP 5MS equipped with a DB-1MS column (30×250 µm, 0.25 μm) were used with gas of helium as a mobile phase (40 mL/min). The temperature program was 50°C for 5 minutes, 5°C/min to 240°C, then 10°C/min to 290°C. The split ratio was 1:50 in this procedure. The injector temperature was modified to 290°C and mass spectra were recorded at 70 eV. The mass range was set up from 35 to 450 m/z. For the injection (split injector), 5 μL of essential oil was diluted in 500 μL of hexane, and 5 μL of this diluted solution was injected. Identification and quantity of O. vulgare’s volatile oil constituents were made on the basis of their retention times, the peak area of spectrograms and their mass spectra, which were confirmed by comparison with data from the Wiley mass spectral library.22
3
Essential Oil Profiling of P. platychlaena Fruits
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EO of P. platychlaena fruits and the fractions were analyzed
by GC-FID and GC/MS using an Agilent GC-mass selective detector (MSD)
system. GC/MS analyses were carried out with an Agilent 5975 GC/MSD
system. An Innowax fused silica capillary column was used with helium
as the carrier gas. The oven temperature was kept at 60 to 240 °C.
The mass spectra were the mass range m/z 35–450.17 (link)GC analyses were
performed by using an Agilent 6890N GC system. The FID detector temperature
was set to 300 °C. Simultaneous autoinjection was done to obtain
equivalent retention times. Relative percentages (%) of the volatiles
were calculated using FID chromatograms. This process was performed
by MassFinder 4 Library, GC/MS Library, in-house “Baser Library
of Essential Oil Constituents” by analyzing either authentic
samples or the relative retention index (RRI) of n-alkanes.18 ,19
by GC-FID and GC/MS using an Agilent GC-mass selective detector (MSD)
system. GC/MS analyses were carried out with an Agilent 5975 GC/MSD
system. An Innowax fused silica capillary column was used with helium
as the carrier gas. The oven temperature was kept at 60 to 240 °C.
The mass spectra were the mass range m/z 35–450.17 (link)GC analyses were
performed by using an Agilent 6890N GC system. The FID detector temperature
was set to 300 °C. Simultaneous autoinjection was done to obtain
equivalent retention times. Relative percentages (%) of the volatiles
were calculated using FID chromatograms. This process was performed
by MassFinder 4 Library, GC/MS Library, in-house “Baser Library
of Essential Oil Constituents” by analyzing either authentic
samples or the relative retention index (RRI) of n-alkanes.18 ,19
4
GC-MS Analysis of Plant Extracts
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The derivatized extracts were evaluated using an Agilent 5975 GC-MSD system on an Innowax FSC column (60 m × 0.25 mm, 0.25 μm film thickness) with helium as the mobile phase (1 mL/min). The GC oven temperature was set at +60 °C for 10 min, then increased to +220 °C at a rate of +4 °C/min. It was then maintained at+220 °C for 10 min before rising again to +240 °C at 1 °C/min. The injections were administered in splitless mode, with the temperature set at +250 °C. An ionization energy of 70 eV was used in the mass spectrometer, which was calibrated to a scanning range of m/z 35–450 atomic mass units. The GC-MS analysis was done twice for accuracy, and the resultant data were averaged.
The compounds’ relative percentages in the hexane and ethanol extracts were determined separately by integrating the mass chromatograms’ peaks. The identification of the extract components was confirmed by comparing the relative incidence indices (RRI) and mass spectra of the n-alkane (C5-C30) series, as detailed in the literature. The comparison utilized the Wiley 8th Ed./NIST 05 Mass Spectra Library, Adams Essential Oil Mass Spectral Library, and Palisade 600k Complete Mass Spectra Library [49 (link)].
The compounds’ relative percentages in the hexane and ethanol extracts were determined separately by integrating the mass chromatograms’ peaks. The identification of the extract components was confirmed by comparing the relative incidence indices (RRI) and mass spectra of the n-alkane (C5-C30) series, as detailed in the literature. The comparison utilized the Wiley 8th Ed./NIST 05 Mass Spectra Library, Adams Essential Oil Mass Spectral Library, and Palisade 600k Complete Mass Spectra Library [49 (link)].
5
GC-MS Analysis of Chemical Compounds
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The GC-MS analysis was carried out with an Agilent 5975 GC-MSD system. Innowax FSC column (60 m × 0.25 mm, 0.25 μm film thickness) was used with helium as the carrier gas (0.8 mL/min). GC oven temperature was kept at 60 °C for 10 min and programmed to 220 °C at a rate of 4 °C/min, and kept constant at 220 °C for 10 min and then programmed to 240 °C at a rate of 1 °C/min. Split ratio was adjusted at 40:1. The injector temperature was set at 250 °C. Mass spectra were recorded at 70 eV. Mass range was from m/z 35 to 450.
6
GC-MS Analysis of Complex Samples
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The GC-MS analysis was carried out with an Agilent 5975 GC-MSD system. An Innowax FSC column (60 m × 0.25 mm, 0.25 mm film thickness) was used with helium as the carrier gas (0.8 mL/min). The GC oven temperature was kept at 60 °C for 10 min and programmed to 220 °C at a rate of 4 °C/min, and kept constant at 220 °C for 10 min and then programmed to 240 °C at a rate of 1 °C/min. The split ratio was adjusted at 40:1. The injector temperature was set at 250 °C. Mass spectra were recorded at 70 eV. The mass range was from m/z 35 to 450 [42 (link)].
7
GC-MS Analysis of Essential Oil Constituents
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The GC-MS analysis was carried out with an Agilent 5975 GC-MSD system. Innowax FSC column (60 m × 0.25 mm, 0.25 μm film thickness) was used with helium as carrier gas (0.8 mL/min). GC oven temperature was kept at 60 °C for 10 min and programmed to 220 °C at a rate of 4 °C/min, and kept constant at 220 °C for 10 min and then programmed to 240 °C at a rate of 1 °C/min. The injector temperature was set at 250 °C. Mass spectra were recorded at 70 eV. Mass range was from m/z 35 to 450. Relative percentage amounts of the separated compounds were calculated from TIC chromatograms.
The identification of the essential oil components was carried out by comparison of their relative retention times with those of authentic samples or by comparison of their relative retention index (RRI) to series of n-alkanes. Computer matching against commercial (Wiley GC/MS Library, MassFinder Software 4.0) (McLafferty and Stauffer, 1989 , Hochmuth, 2008 ) and in-house “Başer Library of Essential Oil Constituents” built up by genuine compounds and components of known oils.
The identification of the essential oil components was carried out by comparison of their relative retention times with those of authentic samples or by comparison of their relative retention index (RRI) to series of n-alkanes. Computer matching against commercial (Wiley GC/MS Library, MassFinder Software 4.0) (McLafferty and Stauffer, 1989 , Hochmuth, 2008 ) and in-house “Başer Library of Essential Oil Constituents” built up by genuine compounds and components of known oils.
8
Quantitative GC-FID and GC-MS Analysis of Essential Oil Constituents
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The Agilent
6890N GC system was used. Simultaneous automatic injection was carried
out using the same conditions in two identical columns [HP-Innowax
FSC column (60 m × 0.25 mm, 0.25
μm film thickness, Agilent, Walt & Jennings Scientific,
Wilmington, Delaware)] in the Agilent 5975 GC/MSD system. Relative
percentages (%) of the compounds were calculated using the FID chromatograms.
For idenfication and characterization in-house “Baser Library
of Essential Oil Constituents” and various GC/MS Libraries
such as MassFinder 3 Library, where authentic samples or the relative
retention index (RRI) of n-alkanes were also considered,29 (link) see, for details,Figures S1 and S2 .
6890N GC system was used. Simultaneous automatic injection was carried
out using the same conditions in two identical columns [HP-Innowax
FSC column (60 m × 0.25 mm, 0.25
μm film thickness, Agilent, Walt & Jennings Scientific,
Wilmington, Delaware)] in the Agilent 5975 GC/MSD system. Relative
percentages (%) of the compounds were calculated using the FID chromatograms.
For idenfication and characterization in-house “Baser Library
of Essential Oil Constituents” and various GC/MS Libraries
such as MassFinder 3 Library, where authentic samples or the relative
retention index (RRI) of n-alkanes were also considered,29 (link) see, for details,
9
GC-MS Analysis of S. tmolea Extracts
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The GC-MS analysis of essential oil and n-hexane extract of S. tmolea was performed with an Agilent 5975 GC-MSD system. An Innowax FSC column (60 m × 0.25 mm, 0.25 μm film thickness) was operated with helium as the carrier gas (0.8 ml/min). GC oven temperature was kept at 60 °C for 10 min and then increased to 220 °C at a rate of 4 °C/min, and maintained at 220 °C for 10 min and then increased to 240 °C at a rate of 1 °C/min. The injector temperature was arranged at 250 °C. Mass spectra were recorded at 70 eV. Mass range was from m/z 35 to 450.
10
GC-MS Analysis of Organic Compounds
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The GC-MS analysis was carried out with an Agilent 5975 GC-MSD system. An Innowax fused silica capillary column (60 m × 0.25 mm, 0.25 μm film thickness) was used with helium as the carrier gas (0.8 ml/min). The GC oven temperature was held at 60 °C for 10 min after injection and then ramped to 220 °C at a rate of 4 °C/min, and held at 220 °C for 10 min, followed by a second ramp to 240 °C at a rate of 1 °C/min. The split ratio was set at 40:1. The injector temperature was set at 250 °C. Mass spectra were recorded using 70 eV electrons in Election Ionization (EI) mode. The mass analyzer was scanned from m/z 35–450 at a scan rate of (3.46) s−1.
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