Rnase a

Manufactured by 4A Biotech

Sourced in China

RNase A is a ribonuclease enzyme that catalyzes the hydrolytic cleavage of single-stranded RNA. It is commonly used in molecular biology and biochemistry laboratories to degrade RNA in various applications, such as RNA purification and RNA preparation for further analysis.

Automatically generated - may contain errors

Lab products found in correlation

Propidium iodide, by 4A Biotech (5 mentions) Fluorescence activated cell sorting flow cytometry, by Beckman Coulter (5 mentions) Annexin 5 pi detection kit, by 4A Biotech (5 mentions) Flow cytometry, by Beckman Coulter (3 mentions) Propidium iodide pi, by 4A Biotech (2 mentions) Facscan, by BD (1 mentions) Annexin 5 fitc pi, by Keygen Biotech (1 mentions) Cellquest 6, by BD (1 mentions) Annexin 5 fitc apoptosis detection kit, by 4A Biotech (1 mentions) Facscalibur flow cytometer, by BD (1 mentions) Ow cytometry, by Beckman Coulter (1 mentions) Uorescence activated cell sorting ow cytometry, by Beckman Coulter (1 mentions)

9 protocols using rnase a

Cell Cycle and Apoptosis Assay

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Cells were seeded into 6-well plates and treated with sorafenib for 24 h. 3 × 10⁵ treated cells were seeded into 6-well plates and cultured for 48 h at 37°C to assess the cell cycle and apoptosis. The cells for cell cycle analysis were digested using trypsin (Hyclone), washed twice with phosphate-buffered saline (PBS), and fixed in 70% ethanol overnight at 4°C. Then the cells were centrifuged at 500 × g for 5 min, washed twice with cold PBS, and centrifuged. Cell cycle analysis was performed through fluorescence-activated cell sorting flow cytometry (Beckman Coulter, Palo Alto, CA, USA) after treating the cells with RNase A (0.1 mg/mL) and propidium iodide (PI, 0.05 mg/mL) purchased from 4A Biotech (Beijing, China) for 30 min at 37°C. Following the instructions of the manufacturer, cells were harvested and were stained with Annexin V-FITC/PI (KeyGEN Biotech, Nanjing, China) for the analysis of apoptosis. Then the cells were acquired by flow cytometry (FACScan, BD Biosciences, USA) and analyzed by FlowJo 7.6.1.

Weng H., Zeng L., Cao L., Chen T., Li Y., Xu Y., Peng Y, & Ye Y. (2021). circFOXM1 contributes to sorafenib resistance of hepatocellular carcinoma cells by regulating MECP2 via miR-1324. Molecular Therapy. Nucleic Acids, 23, 811-820.

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Cell Cycle and Apoptosis Analysis

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SKOV3/PTX and HeyA-8/PTX cells were seeded into six-well plates and treated with PTX for 48 h. To assess the cell cycle and apoptosis, 3 × 10⁵ treated cells were seeded into six-well plates and cultured for 48 h at 37°C. The cells for cell cycle analysis were digested using trypsin (HyClone), washed twice with phosphate-buffered saline (PBS), and fixed in 70% ethanol overnight at 4°C. The cells were centrifuged at 500 × g for 5 min, washed twice with cold PBS, and centrifuged. After treating with RNase A (0.1 mg/mL) and propidium iodide (PI, 0.05 mg/mL) purchased from 4A Biotech (Beijing, China) for 30 min at 37°C, cell cycle analysis was performed through fluorescence-activated cell sorting flow cytometry (Beckman Coulter, Palo Alto, CA, USA). For the analysis of apoptosis, cells were trypsinized followed by two PBS washing steps. The cells were stained using the annexin V/PI detection kit (4A Biotech, Beijing, China) for 5 min at room temperature. The apoptotic cells were measured using flow cytometry (Beckman Coulter). All experiments were repeated at least three times.

Zhang S., Cheng J., Quan C., Wen H., Feng Z., Hu Q., Zhu J., Huang Y, & Wu X. (2019). circCELSR1 (hsa_circ_0063809) Contributes to Paclitaxel Resistance of Ovarian Cancer Cells by Regulating FOXR2 Expression via miR-1252. Molecular Therapy. Nucleic Acids, 19, 718-730.

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Cell Cycle and Apoptosis Analysis by Flow Cytometry

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As previously described,^{[15 (link)]} flow cytometry (FCM) was used to analyze cell cycle and apoptosis. The cell cycle was analyzed by ethanol-fixed cells stained with propidium iodide (PI) in buffer containing RNase A (Cat: FXP0211, 4A Biotech Co., Ltd, Beijing, China). The DNA content was assessed with a FACS Calibur flow cytometer (BD Biosciences, USA). Cell apoptosis was detected using the Annexin V-FITC Apoptosis Detection Kit (Cat: FXP022, 4A Biotech Co., Ltd, Beijing, China). The percentage of apoptotic cells were calculated with CellQuest 6.0 (BD Biosciences, USA).

Gao Z., Wu X., Yang L., Liu C., Wang X., Wang H, & Dong K. (2023). Role of CD5 molecular-like on hepatocellular carcinoma. Chinese Medical Journal, 136(5), 556-564.

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Cell Cycle and Apoptosis Analysis

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SKOV3/PTX and HeyA-8/PTX cells were seeded into 6-well plates and treated with PTX for 48 h. To assess the cell cycle and apoptosis, we seeded 3 × 10⁵ treated cells into 6-well plates and cultured them for 48 h at 37°C. The cells for cell-cycle analysis were digested using trypsin (Hyclone), washed twice with phosphate-buffered saline (PBS), and fixed in 70% ethanol overnight at 4°C. The cells were centrifuged at 500 × g for 5 min, washed twice with cold PBS, and centrifuged. After treatment with RNase A (0.1 mg/mL) and propidium iodide (PI, 0.05 mg/mL) purchased from 4A Biotech (Beijing, China) for 30 min at 37°C, cell-cycle analysis was performed through fluorescence-activated cell sorting flow cytometry (Beckman Coulter, Palo Alto, CA, USA). For the analysis of apoptosis, cells were trypsinized followed by two PBS washing steps. The cells were stained using the Annexin V/PI detection kit (4A Biotech, Beijing, China) for 5 min at room temperature. The apoptotic cells were measured using flow cytometry (Beckman Coulter). All experiments were repeated at least three times.

Xia B., Zhao Z., Wu Y., Wang Y., Zhao Y, & Wang J. (2020). Circular RNA circTNPO3 Regulates Paclitaxel Resistance of Ovarian Cancer Cells by miR-1299/NEK2 Signaling Pathway. Molecular Therapy. Nucleic Acids, 21, 780-791.

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Cell Cycle Analysis by Flow Cytometry

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To assess the cell cycle, we seeded 3 × 10⁵ treated cells into six-well plates and incubated them at 37 °C for 48 h. For the cell cycle analysis, cells were digested with trypsin (Hyclone, Logan, UT, USA), washed twice with phosphate-buffered saline (PBS), and fixed in 70% ethanol at 4 °C overnight. The cells were centrifuged at 500× g for 5 min, washed twice with cold PBS, and centrifuged. Cell cycle analysis was performed using fluorescence-activated cell sorting after the digested cells were treated with RNase A (0.1 mg/mL) and stained with propidium iodide (0.05 mg/mL; 4A Biotech, Beijing, China) for 30 min at 37 °C.

Li M., Gong W., Chen J., Zhang Y., Ma Y, & Tu X. (2023). PPP3R1 Promotes MSCs Senescence by Inducing Plasma Membrane Depolarization and Increasing Ca2+ Influx. International Journal of Molecular Sciences, 24(5), 4421.

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Cell Cycle and Apoptosis Analysis

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For analysis of the cell cycle and apoptosis, 3 × 10⁵ treated cells were seeded in 6-well plates and cultured for 48 h at 37°C. For cell cycle analysis, the cells were digested using trypsin (HyClone, Logan, UT, United States), washed twice with phosphate-buffered saline (PBS), and fixed in 70% ethanol overnight at 4°C. The cells were centrifuged at 500 ×g for 5 min, washed twice with cold PBS, and centrifuged. After the cells were treated with RNase A (0.1 mg/ml) and propidium iodide (PI, 0.05 mg/ml, 4A Biotech, Beijing, China) for 30 min at 37°C, cell cycle analysis was performed using fluorescence-activated cell sorting flow cytometry (Beckman Coulter, Palo Alto, CA, USA). For analysis of apoptosis, the cells were trypsinized followed by two PBS washes. The cells were stained using the Annexin V/PI detection kit (4A Biotech) for 5 min at 25°C. Apoptotic cells were measured using flow cytometry (Beckman Coulter). All experiments were repeated at least three times.

Jiang B., Tian M., Li G., Sadula A., Xiu D., Yuan C, & Bing Y. (2022). circEPS15 Overexpression in Hepatocellular Carcinoma Modulates Tumor Invasion and Migration. Frontiers in Genetics, 13, 804848.

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Cell Cycle Analysis of ccRCC Cells

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ccRCC cells were treated with CVB (40 μM) in a 6-well plate for 48 h. Cells were then collected, washed twice in cold PBS and fixed in 70% ethanol at 4 °C overnight. The next day, the fixed cells were washed with PBS again and then stained with propidium iodide (PI) (40 µg/ml) and RNase A (2.5 mg/ml, 4A Biotech, Beijing, China) at 37 °C in the darkroom for 30 min. A flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA) was used to detect the cell cycle distribution. The assays were repeated three times.

Liu Y., Lv H., Li X., Liu J., Chen S., Chen Y., Jin Y., An R., Yu S, & Wang Z. (2021). Cyclovirobuxine inhibits the progression of clear cell renal cell carcinoma by suppressing the IGFBP3-AKT/STAT3/MAPK-Snail signalling pathway. International Journal of Biological Sciences, 17(13), 3522-3537.

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Cell Cycle and Apoptosis Analysis

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To assess the cell cycle and apoptosis, 3 × 10⁵ treated cells were seeded into 6-well plates and cultured for 48 h at 37 °C. The cells for cell cycle analysis were digested using trypsin (Hyclone), washed twice with phosphate-buffered saline (PBS), and fixed in 70% ethanol overnight at 4 °C. The cells were centrifuged at 500 g for 5 min, washed twice with cold PBS, and centrifuged. After treating with RNase A (0.1 mg/ml) and propidium iodide (PI, 0.05 mg/ml) purchased from 4A Biotech (Beijing, China) for 30 min at 37 °C, cell cycle analysis was performed through fluorescence-activated cell sorting flow cytometry (Beckman Coulter, Palo Alto, CA, USA).
For the analysis of apoptosis, cells were trypsinised followed by two PBS washing steps. The cells were stained using the Annexin V/PI detection kit (4A Biotech, Beijing, China) for 5 min at room temperature. The apoptotic cells were measured using flow cytometry (Beckman Coulter). All experiments were repeated at least three times.

Xu L., Feng X., Hao X., Wang P., Zhang Y., Zheng X., Li L., Ren S., Zhang M, & Xu M. (2019). CircSETD3 (Hsa_circ_0000567) acts as a sponge for microRNA-421 inhibiting hepatocellular carcinoma growth. Journal of Experimental & Clinical Cancer Research : CR, 38, 98.

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Cell Cycle and Apoptosis Analysis

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To assess the cell cycle and apoptosis, 3×105 treated cells were seeded into 6-well plates and cultured for 48h at 37°C. The cells for cell cycle analysis were digested using trypsin (Hyclone), washed twice with phosphate-buffered saline (PBS), and xed in 70% ethanol overnight at 4°C. The cells were centrifuged at 500g for 5min, washed twice with cold PBS, and centrifuged. After treating with RNase A (0.1mg/ml) and propidium iodide (PI, 0.05mg/ml) purchased from 4A Biotech (Beijing, China) for 30min at 37°C, cell cycle analysis was performed through uorescence-activated cell sorting ow cytometry (Beckman Coulter, Palo Alto, CA, USA). For the analysis of apoptosis, cells were trypsinised followed by two PBS washing steps. The cells were stained using the Annexin V/PI detection kit (4A Biotech, Beijing, China) for 5min at room temperature. The apoptotic cells were measured using ow cytometry (Beckman Coulter). All experiments were repeated at least three time.

Tian M., Li G., Jiang B., Sadula A., Xiu D., Yuan C., & Bing Y. (2020). circEPS15 Overexpression in Hepatocellular Carcinoma Modulates Tumor Invasion and Migration.

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