Cd21 pe cy7

Manufactured by BioLegend

CD21 PE-Cy7 is a fluorescently labeled antibody that binds to the CD21 receptor expressed on the surface of B cells and follicular dendritic cells. It can be used for the identification and analysis of these cell types in flow cytometry applications.

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Lab products found in correlation

Cd138 apc, by BioLegend (2 mentions) Cd69 fitc, by BD (1 mentions) Cd31 pe, by BD (1 mentions) Cd56 apc, by BD (1 mentions) Il 17 pe, by Thermo Fisher Scientific (1 mentions) Cd4 fitc, by BioLegend (1 mentions) Cd19 apc cy7, by BioLegend (1 mentions) Cd4 bv421, by BioLegend (1 mentions) Ifn γ fitc, by BD (1 mentions) Il 2 pe, by BioLegend (1 mentions) Cd107a pe, by BD (1 mentions) Cd4 pe cy7, by Beckman Coulter (1 mentions) Cd21 pe cy7, by BD (1 mentions) Cd45 pacific blue, by Beckman Coulter (1 mentions) Vα24 pe, by Beckman Coulter (1 mentions) Vβ11 fitc, by Beckman Coulter (1 mentions) Ccr7 pe, by R&D Systems (1 mentions) Il 4 apc, by Thermo Fisher Scientific (1 mentions) Igm alexa fluor 647, by Jackson ImmunoResearch (1 mentions) Bcl 6, by Leica (1 mentions)

Close spelling variants of this product

Anti-CD21 PE-Cy7 (2 citations)

4 protocols using cd21 pe cy7

Comprehensive Immune Cell Phenotyping

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The following antibodies were used in this study: CD3 AF700, CD4 BV421, CD4 FITC, CD6 APC, CD10 BV605, CD19 APC-Cy7, CD21 PE-Cy7, CD25 PerCP-Cy5.5, CD27 BV421, CD28 PerCP-Cy5.5, CD38 PerCp-Cy5.5, CD45RA APC-Cy7, TCR αβ PerCP-Cy5.5, IL-2 PE, LAT PE, NTAL/LAB APC, and biotin anti–human TCR-Vd2 (BioLegend); TCR-Vd1 APC (Miltenyi Biotec); CD8 APC, CD8 Pacific blue, CD21 PE-Cy7, CD31 PE, CD56 APC, CD69 FITC, CD107a PE, CD127 Alexa Fluor 647, IFN-γ FITC, TCR γδ PE, IgG Alexa Fluor 700, IκBα PE, ERK1/2(pT202/pY204) AF647, and ZAP70(pY319)/SYK(pY352) APC (BD); IgD FITC and IgA PE (SouthernBiotech); CD3 PE-Cy7, CD4 PE-Cy7, CD8 PE, CD16 FITC, CD45RA FITC, CD45 Pacific blue, Vα24 PE, and Vβ11 FITC (Beckman Coulter); CCR7 PE (R&D Systems); Bruton tyrosine kinase/ITK(pY551/pY511) PE, IL-17 PE, IL-4 APC, and ICOS PE (eBioscience); IgM Alexa Fluor 647 (Jackson ImmunoResearch Laboratories, Inc.); and PLCγ1(pY783) and goat anti–rabbit AF647 (Cell Signaling Technology). For immunohistochemistry, CD21 (Dako), CD20 (Invitrogen), CD3 (Cell Marque), CD4 and CD8 (Spring Bioscience), and Bcl-6 (Leica Biosystems) were used. For immunoblotting LAT (sc-7948; Santa Cruz Biotechnology, Inc.), FLAG tag (AHP1074; AbD Serotec), actin (sc-1616; Santa Cruz Biotechnology, Inc.), PLCγ1 (pY783; no. 2821; Cell Signaling Technology), and ZAP70 (pY319; no. 2701; Cell Signaling Technology) were used.

Keller B., Zaidman I., Yousefi O.S., Hershkovitz D., Stein J., Unger S., Schachtrup K., Sigvardsson M., Kuperman A.A., Shaag A., Schamel W.W., Elpeleg O., Warnatz K, & Stepensky P. (2016). Early onset combined immunodeficiency and autoimmunity in patients with loss-of-function mutation in LAT. The Journal of Experimental Medicine, 213(7), 1185-1199.

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Multi-color Flow Cytometry Immune Profiling

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PBMCs were stained immediately after thawing in FACS buffer (PBS with 0.1% NaN₃ and 2% FBS; Lonza). Color compensations were done using a mix of cells and compensation beads (BD Biosciences). After incubation of the cells with antibodies on ice for 30 min, they were washed with FACS buffer. Hoechst (Invitrogen) live/dead marker was added shortly prior to measuring on a FACSAria SORP machine (BD Biosciences). The following antibodies were used in the panel: CD14-eFluor605NC, CD24-eF450, CD43-APC, CD23-APC-eFluor780 (eBioscience), IgD-BV421, CD19-BV605, IgG-PE (BD Pharmingen), CD10-BV510, CD138-BV711, CD27-PECF594 (BD Horizon), IgM-BV570, CD38-PerCP-Cy5.5, CD21-PE-Cy7, CD20-AF700 (BioLegend), CD5-FITC, CD3-PE-Dy647 (Immunotools).

Kirpach J., Colone A., Bürckert J.P., Faison W.J., Dubois A.R., Sinner R., Reye A.L, & Muller C.P. (2019). Detection of a Low Level and Heterogeneous B Cell Immune Response in Peripheral Blood of Acute Borreliosis Patients With High Throughput Sequencing. Frontiers in Immunology, 10, 1105.

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Multiparametric Flow Cytometry of Murine and Human B-Cells

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Single‐cell suspensions were stained with a mixture of 5 to 6 fluorophores including and 7‐aminoactinomycin D (7‐AAD) for viability and acquired on the BD Verse flow cytometer with data analyzed using FlowJo software (BD Biosciences). Human B‐cell subsets were identified using the following antibodies: CD19‐APC, CD27‐BV510, CD38‐APC‐Cy7, CD24‐BV421, and IL‐10‐PE‐Cy7 (all from BioLegend). Mouse B‐cell subsets were identified using the following antibodies: B220‐PE, CD23‐APC, CD21‐PE‐Cy7, IgM‐BV421, CD138‐APC, CD86‐PE‐Cy7, CD69‐APC‐Cy7, and IL‐10‐APC‐Cy7 (all from BioLegend). Intracellular IL‐10 expression was detected as previously reported.³³ Immunophenotype of human and murine B‐cell subsets analyzed are listed in Supplementary Table 1.

Lee W., Wang L., Yen M., Hsu P., Lee Y., Liu K., Lin K., Su Y., Sytwu H, & Yen B.L. (2021). Resident vs nonresident multipotent mesenchymal stromal cell interactions with B lymphocytes result in disparate outcomes. Stem Cells Translational Medicine, 10(5), 711-724.

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Multiparametric Flow Cytometry Analysis of B Cell Subsets

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Cells were stained with panels of antibodies including anti-mouse CD19-pacific Blue, CD138-APC, CD21-PEcy7, CD5-FITC, CD43-PE, IgD-APCcy7, CD38-PE-Cy7, GL-7-Percp (BioLegend, San Diego, CA ), and IgM-APCcy7 (Miltenyi Biotec, Auburn, CA). For the human B cell phenotypes, the following antibodies were used: anti-CD19-pacific blue, anti-CD38-PECy7, anti- CD20-PE, anti-CD27-APCcy7 (BioLegend), and anti– human IgG-PE, anti– human IgA-APC (Miltenyi Biotec). BD Cytofix/ Cytoperm and BD Perm/Wash (BD) were used for IgA and IgG intracellular staining per manufacturers’ instructions. Data were analyzed on a BD Biosciences LSRII machine and Flowjo software 29.

Qu P., Wuest T., Min Y., Alevizos I., Young H.A, & Lin P.C. (2020). Natural Killer Cell Transcript 4 promotes the development of Sjögren’s Syndrome via activation of Rap1 on B cells. Journal of autoimmunity, 116, 102559.

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