Species identification was based on morphological features of the ascomata or conidiomata produced on infected plant tissues and micromorphology, supplemented by cultural characteristics. Cross-sections were prepared by hand using a double-edge blade under a dissecting microscope. More than 10 conidiomata/ascomata, 10 asci and/or 50 conidia/ascospores were measured to calculate the mean size and standard deviation (SD ). Microscopic photographs were captured with a Nikon Eclipse 80i microscope equipped with a Nikon digital sight DS-Ri2 high definition colour camera, using differential interference contrast (DIC ) illumination and the Nikon software NIS-Elements D Package v. 3.00. Adobe Bridge CS v. 6 and Adobe Photoshop CS v. 5 were used for the manual editing. Nomenclatural novelties and descriptions were deposited in MycoBank (Crous et al. 2004 ). Colony diameters were measured and the colony colours described after 3 weeks according to the colour charts of Rayner (1970) .
Nis elements d package v 3
Manufactured by Nikon
Sourced in Japan
NIS-Elements D Package v. 3.00 is a software package designed for microscope image acquisition and analysis. The core function of this package is to provide a comprehensive platform for managing and processing digital images obtained from Nikon microscopes.
Automatically generated - may contain errors
3 protocols using nis elements d package v 3
1
Fungal Identification Protocol Using Morphology
2
Fungal Morphological Characterization
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Descriptions were performed based on morphological features of the ascomata or conidiomata from infected host materials. The macro-morphological photographs were captured using a Leica stereomicroscope (M205 FA) (structure and size of stromata, structure and size of ectostromatic disc and ostioles). Micro-morphological observations (shape and size of conidiophores, asci and conidia/ascospores) were determined under a Nikon Eclipse 80i microscope equipped with a Nikon digital sight DS-Ri2 high definition colour camera, using differential interference contrast (DIC) illumination and the Nikon software NIS-Elements D Package v. 3.00. Adobe Bridge CS v. 6 and Adobe Photoshop CS v. 5 were used for the manual editing. Over 10 conidiomata/ascomata, 10 asci and 30 conidia/ascospores were measured to calculate the mean size/length and respective standard deviations (SD). Colony diameters were measured and the colony features were described using the color charts of Rayner (1970) . Nomenclatural novelties and descriptions were deposited in MycoBank (Crous et al. 2004 ).
3
Fungal Species Identification Protocol
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Species identification was based on morphological features of the ascomata or conidiomata produced on infected plant tissues and micromorphology, supplemented by cultural characteristics. Microscopic photographs were captured using a Leica stereomicroscope (M205 FA) (Leica Microsystems, Wetzlar, Germany) (structure and size of stromata; structure and size of ectostromatic disc and ostioles). Microscopicl observations (shape and size of conidiophores, asci and conidia/ascospores) were determined under a Nikon Eclipse 80i microscope (Nikon Corporation, Tokyo, Japan) equipped with a Nikon digital sight DS-Ri2 high definition colour camera, using differential interference contrast (DIC) illumination and the Nikon software NIS-Elements D Package v. 3.00. Adobe Bridge CS v. 6 and Adobe Photoshop CS v. 5 were used for the manual editing. More than 10 conidiomata/ascomata, 10 asci and 30 conidia/ascospores were measured to calculate the mean size/length and respective standard deviations (SD). Colony diameters were measured and the colony features were described using the colour charts of Rayner (1970) . Nomenclatural novelties and descriptions were deposited in MycoBank (www.mycobank.org; Crous et al. 2004) .
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